In vitro Propagation in Three Varieties of Gerbera (Gerbera jamesonii Bolus.) from Flower Bud and Flower Stalk Explants

Abstract

Three types of tender leaves derived explants such as leaf tip, leaf with mid-rib and leaf blade segments as well as flower buds and flower stalks obtained from three selected varieties of gerbera (Gerbera jamesonii Bolus.) cultivated in Bangladesh were exploited for callus induction and in vitro regeneration of plantlets. Among the explants flower bud and flower stalk were suitable and superior for callus induction and subsequent regeneration of in vitro shoots when cultured on MS supplemented with 5.0 mg/l BAP and 1.0 mg/l NAA. However, highest number of multiple shoots were obtained when the flower bud derived callus was subcultured on MS supplemented with 2.0 mg/l BAP. Variety with red petal showed the best response in producing multiple shoots among the three varieties. Root induction at the base of the in vitro regenerated shoots was tried using full and half the strengths of MS containing different concentrations of IBA. Best root induction was observed on half the strength of MS supplemented with 0.2 mg/l IBA. Following adequate acclimatization the regenerated plantlets were successfully transferred to soil where they grew to maturity and produced flowers. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14203 Plant Tissue Cult. & Biotech. 22(2): 143-152, 2012 (December)

Commercial Multiplication of Gerbera (Gerbera jamesonii Bolus ex. Hooker F.) from Young Capitulum Explants

“…The culture establishment for capitulum explants on MS medium supplemented with 3 mg/l BAP + 0.3 mg/l NAA was found to be the best. The results are similar to findings of various researchers Akter et al, (2012), flower bud and flower stalk explants were suitable and superior for callus induction and subsequent regeneration of in vitro shoots, when cultured in MS supplemented with 5.0 mg/l BAP and 1.0 mg/l NAA. However, highest numbers of multiple shoots were obtained when the flower bud derived callus was subcultured on MS supplemented with 2.0 mg/l BAP.…”

High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

“…In Gerbera jamesonii 7, 8, 9, 10 and 11-d-old ower buds were cultured to induce callus. In all four varieties, 7-9-d-old ower buds showed the best response in terms of both callus induction as well as shoot organogenesis (Akter et al 2012). The auxin 2, 4-D is routinely used for callus induction from various explants in different systems (Rathore et al 2011;Abraham and Thomas 2015;Bala et al 2015;Patricia 2021).…”

“…In Arnebia hispidissima of the various explants like leaf, immature in orescence and mature and immature seeds, employed for callus induction and shoot regeneration, only immature in orescence responded (Phulwaria and Shekhawat 2013). Flower buds were used for micropropagation in some systems (Akter et al 2012;Liao et al 2011;Yang et al 2011). Young tissues like immature in orescence, ower buds etc have high meristematic activity and form excellent material for micropropagation studies (Kackar and Shekhawat 1991;Yadav et al 2009;Głowacka et al 2010).…”

Study on Applicability of Sucrose Ester in Tissue Culture Media

“…A tissue-culture technique in which propagules are cloned from tissue taken from a single plant is known as micro-propagation. Micropropagation of gerbera is being used in many countries from a range of explants (Huang and Chu, 1985;Aswath and Choudhary, 2002;Tyagi and Kothari, 2004;Aswath and Wazneen, 2004;Altaf et al, 2009;Aktar et al, 2012).…”