In vitro propagation of a carvacrol-producing type of Lippia origanoides Kunth: A promising oregano-like herb

Castilho, Caroline Vianna V.; Leitão, Suzana Guimarães; Silva, Vanessa D.; Miranda, Carolina de O.; Santos, Marcelly Cristina da S.; Bizzo, H. R.; Silva, Nina C.B. da

doi:10.1016/j.indcrop.2019.01.006

Cited by 26 publications

(16 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Finally, Castilho et al [ 105 ] investigated the agronomic tool of in vitro production for oregano plants, and the findings indicated that the addition of a plant growth regulator (Kinetin, 4.6 μM) yielded a greater number of compounds. Furthermore, the presence of indole-3-acetic acid (IAA) facilitates a high thymol and carvacrol content.…”

Section: Bioactive Potential Of Mexican Oregano Fractionsmentioning

confidence: 99%

Mexican Oregano (Lippia graveolens Kunth) as Source of Bioactive Compounds: A Review

et al. 2021

View full text Add to dashboard Cite

Lippia graveolens is a traditional crop and a rich source of bioactive compounds with various properties (e.g., antioxidant, anti-inflammatory, antifungal, UV defense, anti-glycemic, and cytotoxicity) that is primarily cultivated for essential oil recovery. The isolated bioactive compounds could be useful as additives in the functional food, nutraceuticals, cosmetics, and pharmaceutical industries. Carvacrol, thymol, β-caryophyllene, and p-cymene are terpene compounds contained in oregano essential oil (OEO); flavonoids such as quercetin O-hexoside, pinocembrin, and galangin are flavonoids found in oregano extracts. Furthermore, thermoresistant compounds that remain in the plant matrix following a thermal process can be priced in terms of the circular economy. By using better and more selective extraction conditions, the bioactive compounds present in Mexican oregano can be studied as potential inhibitors of COVID-19. Also, research on extraction technologies should continue to ensure a higher quality of bioactive compounds while preventing an undesired chemical shift (e.g., hydrolysis). The oregano fractions can be used in the food, health, and agricultural industries.

show abstract

Section: Bioactive Potential Of Mexican Oregano Fractionsmentioning

confidence: 99%

Mexican Oregano (Lippia graveolens Kunth) as Source of Bioactive Compounds: A Review

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Adenine type cytokinin plant growth regulators such as BA and KIN play different roles in many aspects of plant growth, development, anabolic and catabolic stimulation processes in plant cell metabolism (Quadri et al 2012). Kinetin is beneficial for inducing cell development, proliferation and new shoot formation when supplemented to the culture medium (Castilho et al 2019), and it has also been suggested for micropropagation of different Thymus species, for example, T. vulgaris, T. longicaulis (Ozudogru et al 2011), T. cariensis (Ozudogru and Kaya 2012), T. hyemalis (Nordine et al 2013) and T. persicus (Bakhtiar et al 2016).…”

Section: Effect Of Different Cytokines On T Cilicicus Meristem Regenerationmentioning

confidence: 99%

Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)

Kaya

Balcı

Akgüller

et al. 2021

Acta bot. Croat. (Online)

View full text Add to dashboard Cite

Thymus cilicicus is an endemic Eastern Mediterranean element that has aromatic-medicinal properties. Its natural population spreads across gravelly ground and open rocky areas of South and Southwest Anatolia. The current study on in vitro propagation of T. cilicicus focused deeply on environmental applications such as the development of an optimum medium composition for efficient and genetically stable micropropagation and improved preservation procedures for long-time conservation of elite germplasms for further studies. For this purpose, MS and OM media were used individually and in combination with cytokinins, charcoal, AgNO3, Fe-EDDHA, and H3BO3. The raw data were statistically analyzed via the graph kernel method to optimize the nonlinear relationship between all parameters. The optimal proliferation medium for T. cilicicus was OM supplemented with a combination of 10 g L-1 charcoal and 1 mg L-1 KIN and the calculated averages of the best regeneration rate, the best shoot number and the best shoot length were 96.89%, 3 and 1.24 respectively on this medium. The determination of genetic stability of in vitro grown plants on the optimum medium compositions obtained by the graph kernel method was carried out with the use of the ISSR-PCR technique. All the ISSR primers produced a total of 192 reproductive band profiles, none of which were polymorphic. Furthermore, the micropropagated plants were successfully rooted and acclimatized to greenhouse conditions. In this study, we present a graph kernel multiple propagation index which considers all the possible parameters needing to be analyzed. Such an index is used for the first time for the determination of the optimum proliferation medium.

show abstract

“…Kinetin (KIN), for example, is capable of inducing cell proliferation and new adventitious shoot bud formation when added to the culture medium (Fráguas et al, 2004;Castilho et al, 2019), and is also recommended for micropropagation of some woody species, for example, Salix humboldtiana (Pereira et al, 2000), Acacia auriculiformis (Yadav et al, 2016) and A. leucophloea (Sharma et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Kinetin and 6-benzyladenine induce different morphogenetic responses in cotyledonary segments of royal poinciana

et al. 2019

View full text Add to dashboard Cite

Understanding the in vitro performance of royal poinciana explants cultured in media supplemented with different types and concentrations of cytokinins may aid in the optimization of regeneration systems established for this woody species. In the present study we evaluate the in vitro performance of royal poinciana cotyledonary explants cultured in the presence of high concentrations of 6-benzyladenine (BA) and kinetin (KIN). Cotyledonary segments obtained from in vitro germinated seedlings were inoculated in Murashige and Skoog (MS) medium, supplemented with different concentrations (1, 2, 4, 8 mg L-1) of BA or KIN. In the control treatment, no plant growth regulators (PGRs) were added. After 40 days of culture, regardless of the concentration used, the treatments supplemented with BA presented higher calli percentage and fresh mass compared to treatments supplemented with KIN. Adventitious shoots were mainly observed in BA-treatments. Histological analysis showed that adventitious shoots formed at the periphery of callus formed from mesophyll cells in the regions of the explant sectioning. The results obtained provide new information for the establishment of a micropropagation system for royal poinciana, an important ornamental tree species.

show abstract

In vitro propagation of a carvacrol-producing type of Lippia origanoides Kunth: A promising oregano-like herb

Cited by 26 publications

References 38 publications

Mexican Oregano (Lippia graveolens Kunth) as Source of Bioactive Compounds: A Review

Mexican Oregano (Lippia graveolens Kunth) as Source of Bioactive Compounds: A Review

Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)

Kinetin and 6-benzyladenine induce different morphogenetic responses in cotyledonary segments of royal poinciana

Contact Info

Product

Resources

About