In Vitro Propagation of Java, New Guinea, and Java x New Guinea Impatiens

Abstract

In vitro propagation of induced tetraploid Java (J) Impatiens platypetala Lindl. TR6-19, New Guinea (NG) Impatiens sp. ‘Skyrocket’, and 2 interspecific J x NG hybrids, T63-1 and T63-3, was achieved by culturing shoot tips on agar-solidified MS revised medium supplemented with levels of kinetin and NAA. The mean number of shoots per shoot tip increased linearly from 0.3 shoots at 1.39 µM to 8.5 shoots at 139.0 µM kinetin for T63-1, the most responsive genotype. The number of shoots produced by T63-1 and T63-3 were not significantly different, but both hybrids produced significantly more shoots than either ‘Skyrocket’ or TR6-19. Shoot tips produced in vitro rooted readily and were transferred successfully to soil. Chemical name used: 1-naphthaleneacetic acid (NAA).