In Vitro Propagation Protocols and Variable Cost Comparison in Commercial Production for Paulownia tomentosa × Paulownia fortunei Hybrid as a Renewable Energy Source

Abstract

In this elaboration, effective methods of in vitro propagation of a Paulownia tomentosa × Paulownia fortunei hybrid are presented, and the variable costs of commercial production evaluated. Plant regeneration of the P. tomentosa × P. fortunei hybrid was achieved through organogenesis in nodal explants. Different concentrations of BAP (6-benzylaminopurine), 0.2, 0.5, 1 mg/L, and light conditions were investigated. The best results were obtained using a half-strength MS medium containing 0.5 mg/L BAP. In standard light conditions, 2 shoots were grown with 3.5 culturable nodes on each, and in 70% reduced light, 2 new shoots were grown with 6 culturable nodes on each. Rooting was successfully achieved when using a hormone-free half-strength MS medium containing vitamin, and 2% sucrose with 95% efficiency. Acclimatization and survival were shown to be 90% in regenerated plants. The cost of production of a single plant of P. tomentosa × P.fortunei hybrid grown in standard light conditions was $0.084 and $0.082 when grown in 70% reduced light where only variable costs were considered. Two major factors affecting P. tomentosa × P fortunei hybrid micropropagation is labor, materials and chemicals. Focusing on reducing this cost can highly lower plantlet price.

In Vitro Mutagenesis and Propagation of Paulownia Tomentosa (Thumb) for Salt Tolerance

“…In this respect, the WPM, which has a low total ion content but a high sulphate and magnesium concentration to encourage the growth of woody plant tissue, is a medium specifically designed for perennial or woody plants (Nas et al, 2013). Half MS medium contained 20 g/l sucrose and 0.5 mg/l BAP is thought to be the most effective for micro-clonal proliferation, whereas 0.2 mg/l BAP causes plants to grow slowly (Pożoga et al, 2019).…”

“…Therefore, this is the main reason why effective vegetative reproduction methods must be adopted. In comparison to root cuttings or shoot cuttings derived from in vitro cultures, seeds germinate slowly and grow slower (Pożoga et al, 2019). The multiplication of species through tissue culture, with the prospect of simultaneous large seedling quantity planting, is characterized by maximum biological homogeneity, soundness, and low cost, regardless of season, climate, and altitude (Bergmann, 1998).…”

Interaction between Growth Regulators Controls In Vitro Shoot Multiplication in Paulownia and Selection of NaCl-Tolerant Variants

“…Paulownia belongs to the Scrophulariaceae family with 40 chromosomes (2n = 2x = 40) and provides an ideal solution to this problem. It is a fast-growing tree that reaches the harvesting stage in ten years, has good quality timber, requires minimal management and little investment, and tolerates a range of temperate conditions (−10 • C-+45 • C) [1]. Paulownia wood is suitable for many industries, including furniture production, aircraft construction, packing boxes, musical instrument, shipbuilding, plywood, paper, cabinet-making, coffins, molding and secondary metabolites, including flavonoids, with high antioxidant activities [2,3].…”

Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

“…It has also been recorded that there is a higher participation of women in the plant tissue culture industry (Reddy, 2007). However, substantial electricity cost is one of the critical factors that negate the popularisation of plant tissue culture-based enterprise (Pożoga et al, 2019). Plant tissue culture has the potential to be a catalyst for the development in both the agricultural and industrial sectors in the emerging economy (Aladele et al, 2012).…”