In vitro propagation using adventitious buds technique as a source of new variability in chrysanthemum

Zalewska, M.; Lema-Rumińska, Justyna; Miler, Natalia

doi:10.1016/j.scienta.2007.01.019

Cited by 45 publications

(29 citation statements)

References 10 publications

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“…'Richmond', 'Lady Orange' and 'Lady Salmon' obtained via radiomutation (Zalewska et al 2007) were the source of plant material. Purple-flowering 'Richmond' is a stable cultivar in terms of flower colour while 'Lady Orange' and 'Lady Salmon' are periclinal chimeras (Zalewska et al 2007).…”

Section: Plant Materialsmentioning

confidence: 99%

Morphogenetic response of shoot tips to cryopreservation by encapsulation-dehydration in a solid mutant and periclinal chimeras of Chrysanthemum × grandiflorum /Ramat./Kitam.

2017

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Cryopreservation is widely applied to many economically important species excluding chimera plants which are problematic for long-term conservation. Their storage problems can be circumvented only by cryopreserving meristems. This study looked at the morphogenetic response of shoot tips of periclinal chimera chrysanthemum 'Lady Orange' and 'Lady Salmon', as well as the solid mutant 'Richmond', that were cryopreserved by encapsulation-dehydration technique. By applying 10 µM ABA in the preculture medium followed by 4-day-long dehydration treatment, the explant survival reached up to 67%. Besides the stimulation of typical single shoot recovery, cryopreservation led to direct or indirect multiple shoot formation, shoot malformation, as well as inhibited their spontaneous rooting. Microscopic analysis revealed three types of structural damages of shoot tips which can correspond with their morphogenetic response in recovery culture. No influence of cryostorage on the acclimatisation efficiency of the recovered chrysanthemums was observed.

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Section: Plant Materialsmentioning

confidence: 99%

Morphogenetic response of shoot tips to cryopreservation by encapsulation-dehydration in a solid mutant and periclinal chimeras of Chrysanthemum × grandiflorum /Ramat./Kitam.

2017

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“…Callus was formed on explants from all cultivars except 'Viking Freja' (data not shown). Differences in the regeneration capacity between cultivars have been observed in many plant species and between mutants obtained from a single cultivar (Zalewska et al 2007). Teixeira da Silva (2004) reviewed Chrysanthemum biotechnology and listed genotypic differences in 36 papers.…”

Section: Adventitious Shootsmentioning

confidence: 99%

“…Within Asteraceae, adventitious shoot formation from leaf discs has been achieved from e.g. Chrysanthemum 9 grandiflorum (Zalewska et al 2007) and Crepis novoana (Corral et al 2011).…”

Section: Introductionmentioning

confidence: 99%

An efficient protocol for regeneration and transformation of Symphyotrichum novi-belgii

Mørk

Henriksen

Brinch-Pedersen

et al. 2011

Plant Cell Tiss Organ Cult

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A protocol for adventitious shoot formation in Symphyotrichum novi-belgii was developed after investigating the effects of cultivar and hormone combinations. A Murashige and Skoog medium with 1.0 mg l -1 6-benzyladenine induced adventitious shoot formation in 15 out of 19 cultivars. Addition of 0.1 mg l -1 indole-3-acetic acid or naphthaleneacetic acid increased the total number of shoots per explant, but not the number of shoots longer than 1 cm. Addition of dichlorophenoxyacetic acid (2,4-D) promoted callus formation, but inhibited shoot elongation. A transformation system for the two cultivars Victoria Fanny and Victoria Jane was developed by co-cultivation of leaf explants with Agrobacterium tumefaciens. Three bacterial strains (LBA 4404, A281 and C58) all carrying the binary vector, p35S-GUS-INT, and harbouring the uidA gene coding for b-glucuronidase (GUS) were used. Regeneration of transgenic plants after co-cultivation with A281 was independent of cultivar, and all explants produced callus followed by indirect shoot formation. In 'Victoria Fanny' shoots were formed faster and without a callus phase after co-cultivation with LBA 4404 or C58. The highest number of potentially transformed shoots was regenerated after co-cultivation of 'Victoria Fanny' leaf explants with LBA 4404. Integration of the transgenes in the plant genome was confirmed using PCR and Southern blot hybridisation. To verify that the transgenes could be transferred to offspring, crosses were conducted between three transgenic lines of 'Victoria Fanny' and two wild type pollen donors. It was demonstrated that viable seeds were produced and that the uidA gene was inherited.

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“…However, the molecular and genetic basis for this potentially beneficial as well possibly disadvantageous variation is now being investigated in many crops including barley [76], Bermudagrass [77], chrysanthemum [78], pear [79], oil palm [80], rice [81] and tea [82].…”

Section: Somaclonal Variationmentioning

confidence: 99%

Plant Cell Culture – Present and Future

Dunwell

2010

Plant Cell Culture

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Plant cell culture has a long history of development and exploitation that has been reviewed many times [1]. There are also several monographs and textbooks that provide detailed discussion on specific techniques and their application [2, 3]. By necessity therefore, the present review is limited in scope and, for the most part, will focus on recent publications. The various sections below each concentrate on a specific aspect of the technology; these are followed by a summary section relating to commercialization. MicropropagationMicropropagation in vitro is well established as a method to propagate [4], preserve and transport germplasm of many species including horticultural [5-7], medicinal [8, 9] and woody [10,11] plants. It also reduces the risk of moving pathogens and insects with the germplasm owing to the inherent pathogen detection capabilities of aseptic cultures. Since this technology is usually limited to the multiplication of pre-existing meristems and does not involve the regeneration of plants from single cells or tissue, it will not be discussed in detail here.

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In vitro propagation using adventitious buds technique as a source of new variability in chrysanthemum

Cited by 45 publications

References 10 publications

Morphogenetic response of shoot tips to cryopreservation by encapsulation-dehydration in a solid mutant and periclinal chimeras of Chrysanthemum × grandiflorum /Ramat./Kitam.

Morphogenetic response of shoot tips to cryopreservation by encapsulation-dehydration in a solid mutant and periclinal chimeras of Chrysanthemum × grandiflorum /Ramat./Kitam.

An efficient protocol for regeneration and transformation of Symphyotrichum novi-belgii

Plant Cell Culture – Present and Future

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