In vitro radical scavenging activity of two Columbian Magnoliaceae

Abstract

The recent interest in the conservation of the tropical forest is due, at least in part, to the potential economic and health benefits that can be exploited from several plants. This report shows the in vitro antioxidant activity of some fractions isolated from leaves of two Columbian Magnoliaceae, Talauma hernandezii G. Lozano-C and Dugandiodendron yarumalense Lozano. The activity was determined using the radical monocation 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS.+) and the stable free radical 2-2-diphenyl-1-picrylhydrazyl (DPPH.), as part of general biological screening of these plants. The antioxidant capacity obtained from fractions was similar to those of alpha-tocopherol, tert-butylated hydroxyanisole (BHA), and ascorbic acid. The most active scavenger extract was the fraction 7 (TAA = 48.6 mmol Trolox/kg extract and IC50 < or = 0.01 kg extract/mmol DPPH); and the least active was the fraction 1 (TAA = 11.23 mmol Trolox/kg extract and IC50 = 0.21 kg extract/mmol DPPH) all of them isolated from D. yarumalense. These results suggest that these plants can be attractive as source of antioxidant compounds with the ability to reduce radicals like ATBS and DPPH.

Antioxidant Activity and Phytopathogenic Control of Extracts and Fraction from Struthanthus calophyllus A.C.Sm. (Loranthaceae)

“…To quantify the radical scavenging activity of the 10 extracts, the degree of discoloration caused by its components was determined in a methanolic solution of DPPH⋅ (2,2‐diphenyl‐1‐picrylhydrazyl) Aldrich lot #STBH0044, using the Brand‐Williams method, with some modifications [29,30] . A 200 mg/L stock solution of DPPH⋅ in methanol was prepared.…”

Mechanisms of skin aging

“…In fact, instead of looking for a direct effect in skin photoaging, most Colombian studies have been focused on the antioxidant or photo-protective activity of some products. One Colombian report has showed the in vitro antioxidant activity of two types of leaves: The Colombian Magnoliaceae and Talauma hernandezii (95). In such study, the activity of these magnoliaceae was determined using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS·+) and the stable free radical 2-2-diphenyl-1-picrylhydrazyl (DPPH·), and antioxidant properties obtained were similar to those reported from α-tocopherol, tertbutylated hydroxyanisole (BHA), and ascorbic acid (95).…”