In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

Adsul, Avinash A.; Chavan, Jaykumar J.; Gaikwad, Nikhil B.; Gurav, Rajaram V.; Dixit, G. B.; Yadav, S. R.

doi:10.1186/s43141-019-0003-6

Cited by 17 publications

(10 citation statements)

References 10 publications

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“…Medium supplemented with auxin at low concentrations in combination with cytokinin promote the growth and formation of new shoots, consequently, increasing multiplication rate [29]. Adsul et al [30] reported that nodal buds cultured on MS medium supplemented with 2.0 mg/l BAP in combination with 0.5 mg/l IBA resulted in production of maximum number of shoots (17.1 ± 1.2) of Ceropegia mohanramii. In contrast, Fraternale et al [31] reported high concentration of IBA with BAP (1.5 mg/l) in MS medium was suitable for shoot multiplication of Bupleurum fruticosum.…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant

Lijalem

Feyissa

2020

Journal of Genetic Engineering and Biotechnology

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Background Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants. Results Among different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization. Conclusions This micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement.

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Section: Discussionmentioning

confidence: 99%

In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant

Lijalem

Feyissa

2020

Journal of Genetic Engineering and Biotechnology

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“…Our results show that the in vitro germination protocol developed in this study, despite being more expensive and less simple, is efficient. Furthermore, such in vitro germinated plants could be further used for micropropagation and/or directly planted into its natural habitat; such strategies have been successfully reported for numerous endangered species with medicinal use [ 25 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana

Gallia

Sarto

Bongiovanni

2021

Journal of Genetic Engineering and Biotechnology

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Background The Pehuén or Monkey puzzle tree (Araucaria araucana) is an evergreen coniferous tree, which has been historically used for social, medicinal, and nutritional purposes. We have recently showed the value of A. araucana seeds as a rich source of micronutrients and antioxidants. This endemic species present in a reduced area in Argentina and Chile is endangered because of the low germination rate and the overexploitation of its edible seeds. Thus, the massive extraction of its seeds is ecologically non-viable resulting in limited availability of its active metabolites. However, biotechnological approaches are attractive strategies of production of valuable metabolites and healthy specimens of endangered plants. The aim of this work was to develop a protocol for in vitro production of antioxidants derived from A. araucana seeds and to obtain healthy plants by optimized seed germination. Results Calli of Pehuén seeds were induced in Murashige and Skoog medium with different combinations of auxins and cytokinins, in light and dark conditions. Callus from embryonic axes developed in medium with 1 mg/l α-naphthaleneacetic acid and 1.5 mg/l 6-benzylaminopurine in light conditions had efficient biomass production, antioxidant activity, high phenolic, and flavonoid content and no cytotoxicity on mammalian cells. Additionally, 100 % germination was obtained in vitro and healthy plants were acclimatized to non-sterile conditions. Conclusion In conclusion, in vitro culture of A. araucana could provide new and sustainable options for production of its valuable metabolites with possible therapeutic and nutritional uses. Also, optimized plant germination and acclimatization of endangered species can contribute to the preservation of pristine environments.

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“…The shoots grown on BAP and IAA containing media were more vigorous, than those developed in PGR supplementation. A synergistic effect of BAP along with various auxins on bud proliferation and multiple shoot formation has been reported for several plant species including Hemidesmus indicus (Sreekumar et al 2000), Huernia hystrix (Amoo et al 2009), Ceropegia panchganiensis (Chavan et al 2013), and Ceropegia mohanramii (Adsul et al 2019), another members of Apocynaceae. A combination of BAP and KN exhibited symptoms of hyperhydricity and reduced shoot elongation.…”

Section: In Vitro Propagationmentioning

confidence: 94%

Morphological characterization, propagation strategy and identification of Boucerosia frerei accessions for their hanging basket exploitation- an attractive succulent with star-shaped flowers from the Northern Western Ghats of India

Bodhe

Patil

Bhosale

et al. 2022

Preprint

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Boucerosia frerei (G. D. Rowley) Mieve & Liede (formerly Frerea indica; family-Apocynaceae) is one of the little known, endemic and critically endangered succulent herbs with pretty flowers distributed sparsely in the Northern Western Ghats of India. Naturally, the plant shows phenotypic variation in numerous qualitative and quantitative characters especially stem, leaves and flowers. Consequently, this is challenging and stimulating taxa that merit considerable further attention of taxonomists, horticulturalist, gardeners, plant enthusiast etc. The present investigation describes the first report of collection, phenotypic characterization, commercially practicable propagation strategy development and identification of the accession for their usage in hanging baskets for B. frerei. The results highlighted some of the important and interesting features of this plant. A total of 32 morphological traits (9 qualitative and 23 quantitative) were noted for five accessions collected from Kate’s Point, Mahabaleshwar (KP), Meruling, Satara (MS), Sajjangad, Satara (SS), Purandar, Pune (PP) and YCIS, Satara (YCIS) and evaluated using ANOVA, PCA and cluster analysis. Ten morphological traits viz. plant height, stem color, number of nodes, number of roots, number of leaves, number of flowers, size, shape, architecture and seed weight contributed to the variation between the accessions. For succulents to be used for hanging baskets, plant height, number of leaves and floral and floral attributes (number, color, size, shape and architecture) are among the most important selection traits. Morphological characterization identified the Meruling, Satara (MS) as the potential accession for hanging baskets. A combination of soil, coconut coir and vermiculite (3:1:1) escalated the phenotypic traits in the aerial parts in hanging basket cultivation. Among the propagation techniques employed, vegetative propagation served superior over tissue-culture technique for large-scale propagation for its conservation and availing the stock material to floriculture industry.

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In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

Cited by 17 publications

References 10 publications

In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant

In vitro propagation of Securidaca longipedunculata (Fresen) from shoot tip: an endangered medicinal plant

Sustainable and efficient protocols for in vitro germination and antioxidants production from seeds of the endangered species Araucaria araucana

Morphological characterization, propagation strategy and identification of Boucerosia frerei accessions for their hanging basket exploitation- an attractive succulent with star-shaped flowers from the Northern Western Ghats of India

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