In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Abstract

Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion:The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations

Synthetic seed technology for encapsulation and regrowth of in vitro-derived shoot-tips and somatic embryos of Asparagus officinalis L

“…The conversion ability of seed storage at 25 °C will have greater reduction compared to 4 °C (Figure 1). These results are in line with reports of Islam and Bari (2012) about the Mentha arvensis plant.…”

“…The germination percentage of uncovered apical buds reached to zero after 60 days and these seeds lost their viability. Based on the results of the study of Islam and Bari (2012) on Mentha arvensis plant, encapsulated apical buds will lose their viability after 30 days of storage and lose their viability after 45 days of encapsulated nodal explants storage at the temperature of 20 ± 2 °C (Islam & Bari, 2012). On this basis, the viability of seeds obtained from Asparagus is higher than Mentha arvensis plant in the long-term storage at 25°C.…”

Optimización de las condiciones de producción de cápsulas con microestacas para aumentar el tiempo de almacenamiento de la planta medicinal mexicana Castilleja tenuiflora (Orobanchaceae)

“…En este estudio, el medio de conversión no fue enriquecido con nutrientes ni reguladores de crecimiento para observar de forma directa el potencial de la semilla sintética para obtener plantas debido a que la conversión es el aspecto más importante de esta tecnología para su uso comercial (Standardi y Piccioni, 1997). Standardi y Piccioni (1997) dicen que el medio de conversión puede optimizarse con reguladores de crecimiento vegetal (RCV) o aumentando otras fuentes como la de carbono y elementos nutritivos (Sundararaj et al, 2010), así como antibióticos, herbicidas, biocontroladores y biofertilizantes, entre otros (Islam y Bari, 2012).…”

“…Con el fin de prolongar el tiempo de almacenamiento de las semillas sintéticas de C. tenuiflora se probaron tres temperaturas de almacenamiento (1.5, 4 y 25 °C) (Islam y Bari, 2012). A estas temperaturas, se evaluó la viabilidad (Fig.…”

In vitro Conversion of Alginate-encapsulated Nodal Segments of Rosa hybrida L. `Kardinal´ Into Complete Plantlets

“…Recently, for encapsulation, mostly Ca-alginate is used for its gel-ling properties and the suitability for plant material (Saiprasad, 2001, Islam and Bar, 2012and Rihan et al, 2017. The beads formed after dropping the Na-alginate in CaCl 2 solution (75 to 100 mM) are wet, sticky and not protected against water loss by evaporation.…”