In vitro regeneration protocol for indica rice genotypes

Abstract

Embryogenic calli from mature seeds of four indica rice genotypes were used to observe their regeneration potentiality and establish a suitable in vitro plantlet regeneration protocol. MS medium supplemented with different phytohormone combinations were used to observe the callus induction ability of the explant. The highest callus induction (73.19%), biggest size of callus (3.133mm) and higher callus weight (0.7167g) were observed in Binadhan-6 in MS medium supplemented with 1.0 mg L-1 2,4-D over all the genotypes and MS medium supplemented with 1.5 mg L-1 2,4-D was the best over all the treatments (66.83%). Among the phytohormone combinations, MS + 8 mg L-1 Kinetin + 0.5 mg L-1 NAA showed the highest shoot regeneration (50.67%) and shoot length (13.7cm). Among the genotypes, Binadhan-6 was highly responsive to shoot regeneration (55.83%). The best root formation from regenerants (87.889%), maximum number of roots per plant (20) and the highest length (4.467 cm) of roots were in MS media supplemented with 0.5 mg L-1 IAA in Binadhan-6. In pot and soil, Binadhan-6 showed the highest survival rate of the plantlet 91.30% and 85%, respectively. This callus induction and in vitro regeneration protocol will be widely applicable for the tissue culture of indica rice.