In vitro regeneration protocol through direct organogenesis for Jatropha curcas L. (Euphorbiaceae) accessions in Ethiopia

Fufa, Hundessa; Tesema, Meseret; Daksa, Jiregna

doi:10.5897/ajb2018.16914

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…According to Shrivastava and Banerjee (2008), MS media supplemented with 3.0 mg/L BAP and 1.0 mg/L IBA resulted in the induction of 100 shoots from a single explant [ 28 ]. Nodal explants, when cultured onto MS medium supplemented with 1.0 mg/L BAP and 0.5 mg/L IBA, showed a higher response of 86–90% for shooting [ 29 ]. Shoots were induced from axillary buds when cultured onto MS media supplemented with 2.8 µM IAA and 13.93 µM Kinetin (KN) [ 30 ].…”

Section: Regeneration Studiesmentioning

confidence: 99%

Biotechnological Research Progress in Jatropha, a Biodiesel-Yielding Plant

Al-Khayri

Sudheer

Preetha

et al. 2022

Plants

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Environmental pollution is one of the most pressing challenges in today’s world. The main cause of this pollution is fuel emissions from automobiles and other sources. As industrialization progresses, we will be unable to compromise on the use of energy to power heavy machines and will be forced to seek out the best options. As a consequence, utilizing green fuel, such as biodiesel derived from natural sources, is a realistic option. Jatropha curcas L. (Euphorbiaceae) is recognized as the greatest feedstock for biodiesel production throughout the world, and it has gained a huge market value in the recent years. Conventional cultivation alone will not be sufficient to meet the global need for the plant’s biomass for the production of biodiesel. Adoption of plant tissue culture techniques that improve the biomass availability is an immediate need. The present review provides detailed information regarding in-vitro plant propagation (direct and indirect organogenesis), somatic embryogenesis, and acclimatization protocols of plantlets for stabilized production of biomass. The review also focuses on biotechnological approaches such as gene transformation studies, production of haploids, and double haploids for developing elite germplasm for high biomass and improved traits for the production of biodiesel.

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Section: Regeneration Studiesmentioning

confidence: 99%

Biotechnological Research Progress in Jatropha, a Biodiesel-Yielding Plant

Al-Khayri

Sudheer

Preetha

et al. 2022

Plants

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“…In general, a productive genetic transformation system is established on the basis of efficient tissue culture regeneration system. However, the cycle of plant regeneration of J. curcas has been reported to be extremely long, which may take 90-140 days to obtain the whole regenerative plants, and the regeneration efficiency of adventitious buds is relatively low (Datta, Mukherjee, Ghosh, & Jha, 2007;El-Sayed, Aly, Mohamed, & Rady, 2020;Fufa, Tesema, & Daksa, 2019;Kalimuthu, Paulsamy, Senthilkumar, & Sathya, 2007;Khuranakaul, Kachhwaha, & Kothari, 2010;Kumar, Anand, & Reddy, 2011;Rajore & Batra, 2005;Shrivastava, & Banerjee, 2008;Sujatha & Mukta, 1996;Wei et al, 2004). Therefore, it is necessary to establish a new and efficient tissue culture regeneration system, which will lay a foundation for the genetic transformation of J. curcas.…”

Section: Introductionmentioning

confidence: 99%

Effect of different factors on plant regeneration from leaf explants of Jatropha curcas L.

Liu

Chen

et al. 2020

Agronomy Journal

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The in vitro regeneration frequency was commonly low, and the quality of adventitious buds was poor, when the traditional methods were used to induce adventitious buds from leaf explants of Jatropha curcas L. through the medium containing low concentration of thidiazuron (TDZ). When treated with high concentrations (6.5-104 mg/L) of TDZ for a certain time (7-120 min), the regeneration frequency and quality of adventitious buds were significantly increased. The best induction rate of adventitious buds (78.58%) and the average number of buds per explant (7.26) were obtained by soaking leaf explants in 26 mg/L TDZ solution for 30 min and then transferring them onto MS medium without hormone for 30 days. Furthermore, the adventitious bud regeneration was affected obviously by source of explants, sterilization time, illumination time and genotype. The subsequent elongation and rooting experiments showed that adventitious bud elongation could be improved by adding diethyl aminoethyl hexanoate (DA-6) in the medium, and the best elongation effect could be gained when the concentration of DA-6 was 1.2 mg/L. The elongated shoots induced the roots and developed into small intact plants on the MS medium with 1.5 mg/L sodium nitroprusside (SNP). After domestication, these small plants could be transplanted into the soil and growing normally. Therefore, the method established in this study was helpful to improve the plant regeneration efficiency of leaf explants in J. curcas.

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An effective method for Agrobacterium tumefaciens -mediated transformation of Jatropha curcas L. using cotyledon explants

Liu

Yang²,

Zhao

et al. 2020

Bioengineered

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Jatropha curcas is one of oilseed crops and has been considered as an energy crop. In the present study, efficient plant regeneration protocol and transformation method were developed for J. curcas . Because the regeneration efficiency of adventitious bud from cotyledon explants of J. curcas induced by traditional methods is low, and it takes a long time to get complete plants. It is necessary to establish a new regeneration system to improve regeneration efficiency. Cotyledon explants were dipped into TDZ solution at different concentrations respectively for various times to obtain higher efficiency of adventitious bud regeneration. This new regeneration method was then applied to genetic transformation of J. curcas . Cotyledon explants were precultured for 1 day after treated with high concentration of Thidiazuron (TDZ) solution (20 mg/L for 40 min), followed by Agrobacterium tumefaciens infection. After co-cultured for 2 days, the explants were placed on the induction hormone-free media for bud regeneration and resistant screening. After 30 days, selected shoot buds were transferred onto elongation medium for 15 days. Young leaf sections of the regenerated shoots were used for PCR (Polymerase chain reaction) detection of the transgenic shoots. The PCR positive shoots were isolated and used for in vitro grafting. The intact plants were obtained within 20 days. GUS (β-Glucosidase) staining and Southern analysis confirmed the transformation events. Briefly, a transformation efficiency of 34.32% was achieved and an intact transgenic plant could be obtained within 65 days.

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In vitro regeneration protocol through direct organogenesis for Jatropha curcas L. (Euphorbiaceae) accessions in Ethiopia

Cited by 5 publications

References 36 publications

Biotechnological Research Progress in Jatropha, a Biodiesel-Yielding Plant

Biotechnological Research Progress in Jatropha, a Biodiesel-Yielding Plant

Effect of different factors on plant regeneration from leaf explants of Jatropha curcas L.

An effective method for Agrobacterium tumefaciens -mediated transformation of Jatropha curcas L. using cotyledon explants

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