In vitro release and cytotoxicity activity of 5-fluorouracil entrapped polycaprolactone nanoparticles

Preparation, Characterization and In vitro Biological activity of 5-Fluorouracil Loaded onto poly (D, L-lactic-co-glycolic acid) Nanoparticles

“…In the second step, the primary emulsion (W1/O) was added in the outer aqueous phase (W2) containing stocks (A, B and C) PVA solutions as stabilizer with homogenization to achieve the double emulsion (W1/O/W2) [30,31]. Also, a stable double emulsion was obtained using homogenization speeds of 20,000 rpm at 5 min for the first emulsion and 21,000 rpm at 15 min for the second emulsion determined to prepare PNs providing the lowest particle size [32]. This mixture was properly homogenized at 21,000 rpm for 15 min by using digital high speed homogenizer (T-10; model).…”

“…In order to determine the EE% of 5-FU in the prepared nanoparticles, the combined washings after centrifugation were appropriately diluted using 0.5% acetic acid. The amount of free, unencapsulated 5-FU was measured spectrophotometrically at 265.2 nm using the regression equation of the standard calibration curve plotted employing suitable concentrations of 5-FU [32]. The amount of 5-FU encapsulated in PLGNPs w was calculated by subtracting the total drug added during the preparation of the PLGNPs from the free drug present in the supernatant from using the following formula [34,38]…”

“…At predetermined time intervals (1, 2, 3, 4, 6, 8, 24 and 48 h), 5 ml of the release medium was withdrawn and replaced with 5 ml of fresh buffer solution. The samples were adequately diluted and analyzed for 5-FU content spectrophotometrically at 266.4 nm [32,34]. The cumulative percentage of drug released was determined as the ratio of the amount of released 5-FU to the amount of 5-FU initially inserted into the dialysis bag.…”