In vitro rhizome induction and plantlet formation from multiple shoots in Bambusa bambos var. gigantea Bennet and Gaur by using growth regulators and sucrose

Kapoor, Priyanka; Rao, I. Usha

doi:10.1007/s11240-005-9074-y

Cited by 26 publications

(22 citation statements)

References 10 publications

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“…In our study, the cytokinins BA and Kin, in conjunction with the auxin IBA, enhanced the multiplication rate, increased the shoot length, and improved the overall health of the shoots. A similar synergistic effect of the cytokinin-auxin combination has been reported in Bambusa bambos (Kapoor and Rao 2006), B. tulda (Mishra et al 2008), and Dendrocalamus longispathus (Saxena and Bhojwani 1993) as well.…”

Section: Discussionsupporting

confidence: 80%

In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation

Negi

Saxena

2010

Plant Biotechnol Rep

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This communication describes for the first time an efficient and reproducible protocol for large-scale multiplication of Bambusa nutans. Nodal segments collected from field-grown clumps and cultured on Murashige and Skoog (MS) medium supplemented with 4.4 lM benzylaminopurine (BA) and 2.32 lM kinetin (Kin) gelled with 0.2% gelrite yielded 80% aseptic cultures with 100% budbreak. The in vitro-formed shoots obtained after bud-break were successfully multiplied in MS liquid medium supplemented with 13.2 lM BA, 2.32 lM Kin, and 0.98 lM indole-3-butyric acid (IBA). Sub-culturing of shoots every 3 weeks on fresh multiplication medium yielded a consistent proliferation rate of 3.5-fold. Shoot clusters containing three to five shoots were successfully rooted with 100% success on half-strength MS liquid medium supplemented with 9.8 lM IBA, 2.85 lM indole-3-acetic acid (IAA), 2.68 lM naphthaleneacetic acid (NAA), and 3% sucrose. Plantlets grown in vitro were acclimatized and subsequently transferred to the field. Inter-simple sequence repeat analysis has confirmed the genetic uniformity of the tissue-cultured plants up to 27 passages.

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Section: Discussionsupporting

confidence: 80%

In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation

Negi

Saxena

2010

Plant Biotechnol Rep

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“…Auxin is involved in rhizome initiation as auxin is required to produce rhizomes in culture, and treatment of plants with auxin transport inhibitors prevents the initiation of rhizomes (Kapoor and Rao, 2006). The levels of various hormones, including auxin and cytokinin, vary in the rhizome during the life cycle of the plant (Maslova et al, 2007).…”

Section: The Role Of Hormones In the Regulation Of Rhizomes In Perennmentioning

confidence: 99%

Hormonal Regulation of Branching in Grasses

2009

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“…Success in shoot multiplication from nodal segments has also been reported in other bamboo species namely Guadua angustifolia (Jimenez et al 2006), Bambusa bambos var. gigantea (Kapoor and Rao 2006), B. vulgaris var. 'Striata' (Ramanayake et al 2006), B. tulda, B. vulgaris (Saxena 1990), D. hamiltonii (Sood et al 2002), and D. giganteus (Ramanayake and Yakandawala 1997).…”

Section: Multiplication Of Culturesmentioning

confidence: 99%

Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers

et al. 2010

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Bambusa nutans Wall., is an evergreen, perennial, and multipurpose bamboo having strong culms, which are largely used for construction, scaffolding, craft purposes, pulp, and paper industry. Multiple shoots from nodal segments (3-4 cm) of young branches of mature culms were established in Murashige and Skoog (1962) (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) (1.0-6.0 mg l -1 ) or in combination with a-naphthaleneacetic acid (NAA) (0.5-1.0 mg l -1 ) or kinetin (Kn) (1.0-2.0 mg l -1 ). February-March and December were found to be the best seasons for culture establishments. Maximum shoots were achieved on MS medium fortified with BAP (2.0 mg l -1 ). Embryogenic callus (slightly greenish compact, globular, and slow growing) was initiated from the base of severed sprouted buds in 2-3 subsequent subcultures on MS medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (5.0 mg l -1 ) under dark incubations. Maturation and germination of well-organized somatic embryos was achieved on MS medium containing BAP and 2,4-D (1.0 mg l -1 each) with 20.0 mg l -1 ascorbic acid. Fullstrength MS medium supplemented with 2% glucose favored further development of proliferated somatic embryos into plantlets. Genetic variations of fieldestablished B. nutans plants regenerated through tissue cultures were assessed by amplified fragment length polymorphism (AFLP) analysis using 6 primer combinations. Four hundred and seven scorable fragments were amplified, of which 402 (98.8%) have recorded conservation at various morphogenetic stages leading to plantlets regeneration, therefore, revealed a high level of genetic stability.

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In vitro rhizome induction and plantlet formation from multiple shoots in Bambusa bambos var. gigantea Bennet and Gaur by using growth regulators and sucrose

Cited by 26 publications

References 10 publications

In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation

In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation

Hormonal Regulation of Branching in Grasses

Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers

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