In vitro ruminal undegradable proteins of alfalfa cultivars

Tremblay, Gaëtan F.; Michaud, R.; Bélanger, Gilles; McRae, K. B.; Petit, H.V.

doi:10.4141/p99-113

Cited by 21 publications

(31 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Of these cultivars, Rangelander had low, and Oneida VR had high, silage NPN concentration at both spring growth and summer regrowth. In a previous paper, Rangelander was identified as rich, and Oneida VR as poor, in forage RUP (Tremblay et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Cultivar differences in maturity were less than 3 d and we considered them negligible. Plot management, details on the conduct of the field experiment, and RUP determination in forage samples are described in Tremblay et al (2000). The DMY and RUP concentrations were presented in this previous paper for four harvests; they were calculated for three harvests in the present paper.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Proteolysis in alfalfa silages made from different cultivars

Tremblay¹,

Bélanger²,

McRae³

et al. 2001

Can. J. Plant Sci.

Self Cite

View full text Add to dashboard Cite

R. 2001. Proteolysis in alfalfa silages made from different cultivars. Can. J. Plant Sci. 81: 685-692. During silage fermentation, proteolysis reduces the nutritional value of N, particularly in alfalfa. This study evaluated the proteolysis of 27 alfalfa cultivars seeded in triplicate in 2 consecutive years. Forage from each plot was wilted to a targeted dry matter (DM) content of 250 g kg -1 and ensiled in laboratory silos. Minisilos were made with spring growth and summer regrowth in the first year of production for the first seeding, and with the first 2 yr of production for the second seeding, giving 3 harvest years altogether. Concentrations of NPN, NH 3 , FAA, DM, TN, and pH were measured in silages, whereas RUP concentration was evaluated in forages. Low NPN concentration indicates low proteolysis during silage fermentation. Spring growth and summer regrowth were analyzed separately by ANOVA as a completely randomized block design replicated over 3 harvest years. A principal component analysis was then performed on the ANOVA means. Silage DM was used as covariate when it was deemed appropriate. There was a significant variation among cultivars for NPN concentration in silages made of summer regrowth; it varied from 612 to 717 g kg -1 of TN. Concentration of NPN was significantly lower for cultivars WL 225, Rangelander, Iroquois, and WL 222, and higher for cultivars Oneida VR, Arrow, Vernal, Spredor 2, and Armor. Oneida VR and Rangelander had respectively high and low silage NPN concentration in both spring growth and summer regrowth. From cultivars with low silage NPN concentration, Rangelander was the only one with high forage RUP concentration in both spring growth and summer regrowth; proteins in this cultivar would be more resistant to microbial degradation during ensiling and in the rumen.Key words: Non protein nitrogen; proteolysis; alfalfa silage; cultivars Tremblay, G. F., Bélanger, G., McRae, K. B. et Michaud, R. 2001. Protéolyse dans l'ensilage de luzerne fait de différents cultivars. Can. J. Plant Sci. 81: 685-692. Au cours de la fermentation du fourrage en ensilage, la protéolyse réduit la valeur nutritive de l'azote, et ce particulièrement chez la luzerne. Cette étude avait pour but d'évaluer la protéolyse chez 27 cultivars de luzerne semés en triplicata au cours de deux années consécutives. Le fourrage de chaque parcelle était séché jusqu'à un taux visé de matière sèche (MS) de 250 g kg -1 puis ensilé en mini-silos. L'ensilage a été fabriqué en première et deuxième coupe de la première année de production du premier semis et au cours des deux premières années de production du deuxième semis; ce qui représente un total de trois années de récolte. Les concentrations en azote non protéique (NPN), ammoniaque (NH 3 ), acides aminés libres (FAA), MS, azote total (TN) et le pH ont été mesurés dans l'ensilage alors que la concentration en protéines non dégradables dans le rumen (RUP) a été estimée dans le fourrage. Une faible concentration en NPN était considérée comme un indicateur d'une faible pr...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Proteolysis in alfalfa silages made from different cultivars

Tremblay¹,

Bélanger²,

McRae³

et al. 2001

Can. J. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Flaxseed samples from each treatment were ground through a 1-mm screen (Wiley mill, Standard Model 3, Arthur H. Thomas Co., Philadelphia, PA) and analyzed for IVDMD (Tilley and Terry 1963), in vitro protein degradation rate and RUP concentration using an inhibitor in vitro procedure (Broderick 1987) as described by Tremblay et al (2000) after 4 h of incubation in rumen fluid. Determinations of IVDMD and RUP were carried out in duplicate and triplicate, respectively, on consecutive weeks.…”

Section: Chemical Analysesmentioning

confidence: 99%

Ruminal biohydrogenation of fatty acids, protein degradability, and dry matter digestibility of flaxseed treated with different sugar and heat combinations

Petit¹,

Tremblay²,

Tremblay³

et al. 2002

Can. J. Anim. Sci.

View full text Add to dashboard Cite

P. 2002. Ruminal biohydrogenation of fatty acids, protein degradability, and dry matter digestibility of flaxseed treated with different sugar and heat combinations. Can. J. Anim. Sci. 82: 241-250. Two experiments were conducted to study the effects of processing techniques on in vitro ruminal undegradable protein (RUP), in vitro ruminal biohydrogenation of fatty acids, and in vitro dry matter digestibility (IVDMD) of rolled flaxseed. In the first experiment, the influence of micronization treatment (130°C for 4 h, 140°C for 2 h, 150°C for 1 h, and 160°C for 0.5 h) was examined. The IVDMD significantly decreased when heating at 150°C for 1 h and 160°C for 0.5 h. Degradation rate of crude protein (CP) decreased and RUP concentration increased in parallel with heating temperature. Micronization had little effect on biohydrogenation of fatty acids. In the second experiment, the influence of sugar addition [none, soaked in a high fructose corn syrup (HFCS) or a lignosulfonate solution] before heating, either in an autoclave or a forced-air oven, at two temperatures (100 or 120°C), and two temperature holding times (30 and 60 min) was examined. Increased temperature and heating time decreased degradation rate of CP and increased the RUP fraction of flaxseeds. There was a decrease in IVDMD with increased temperature when fructose was added. Moist heating is a better option than dry heating, but it has little advantage over not heating to prevent ruminal biohydrogenation of polyunsaturated fatty acids of flaxseeds.

show abstract

“…In alfalfa, variations for RUP concentration in wholeplant have been reported among genotypes (Broderick and Buxton 1991;Skinner et al 1994) and cultivars (Griffin et al 1994;Guines et al 2000;Tremblay et al 2000). Rooney et al (1997) concluded that genetic effects for alfalfa protein degradability do exist and they are mostly additive.…”

mentioning

confidence: 99%

Leaf and stem dry matter digestibility and ruminal undegradable proteins of alfalfa cultivars

Tremblay¹,

Bélanger²,

McRae³

et al. 2002

Can. J. Plant Sci.

Self Cite

View full text Add to dashboard Cite

R. 2002. Leaf and stem dry matter digestibility and ruminal undegradable proteins of alfalfa cultivars. Can. J. Plant Sci. 82: 383-393. Increased dry matter digestibility and ruminal undegradable protein (RUP) concentration are traits highly ranked to improve the nutritive value of forage legumes. Whole-plant, leaf, and stem samples of 27 alfalfa cultivars grown in eastern Canada were analysed for total nitrogen (TN), in vitro RUP, and in vitro dry matter digestibility (IVDMD). The objectives were to determine if significant variation exists among these cultivars, and to establish the relationship between nutritive value parameters of leaves and stems, and those of whole plants. Cultivars were seeded in triplicate on 2 consecutive years and evaluated during the 2 subsequent production years with two harvests per year. There were significant differences among cultivars in IVDMD of stems and plants in spring growth and summer regrowth. Cultivar differences in leaf IVDMD were significant only in spring growth, but this variation was negligible in practical terms. Cultivars with high forage IVDMD also had high stem IVDMD and this relationship was stronger in summer regrowth. Alfalfa plant IVDMD is a function of stem digestibility and the leaf weight ratio (LWR). Cultivar differences were significant for plant TN in spring growth and summer regrowth, and for stem TN in spring growth only. Leaf (270 to 306 g kg -1 TN) and stem (242 to 287 g kg -1 TN) RUP concentrations varied among cultivars in spring growth. Cultivars with high stem RUP also had high leaf RUP concentration but differences in plant RUP were not always attributed to leaf and stem RUP. The RUP concentration was on average 10% higher in leaves than in stems in spring growth, and this difference reached 15% in summer regrowth. The LWR varied among cultivars in spring growth and summer regrowth, and it was positively related to plant RUP in spring growth. Improved whole-plant RUP concentration is unlikely to be achieved by simply selecting for high RUP in leaves or stems; LWR has a principal role in determining alfalfa plant RUP concentration.Key words: Dry matter digestibility, ruminal undegradable proteins, alfalfa, genetic variation, stems, leaves Tremblay, G. F., Bélanger, G., McRae, K. B. et Michaud, R. 2002. Digestibilité de la matière sèche et protéines non dégrad-ables dans le rumen des feuilles et des tiges de cultivars de luzerne. Can. J. Plant Sci. 82: 383-393. Une digestibilité de la matière sèche et une concentration en protéines non dégradables au niveau du rumen (RUP) élevées sont parmi les premiers critères permettant d'améliorer la valeur nutritive des légumineuses fourragères. Les plantes, les feuilles et les tiges de 27 cultivars de luzerne cultivés dans l'est du Canada ont été analysés pour leur concentration en azote total (TN) et en RUP, ainsi que pour la digestibilité in vitro de la matière sèche (IVDMD). Le but était de déterminer s'il existe une variation significative entre les cultivars, et d'établir la relation entre les paramè...

show abstract

In vitro ruminal undegradable proteins of alfalfa cultivars

Cited by 21 publications

References 29 publications

Proteolysis in alfalfa silages made from different cultivars

Proteolysis in alfalfa silages made from different cultivars

Ruminal biohydrogenation of fatty acids, protein degradability, and dry matter digestibility of flaxseed treated with different sugar and heat combinations

Leaf and stem dry matter digestibility and ruminal undegradable proteins of alfalfa cultivars

Contact Info

Product

Resources

About