In vitro scavenging activity for reactive oxygen and nitrogen species by nonsteroidal anti-inflammatory indole, pyrrole, and oxazole derivative drugs

2-Styrylchromones: Novel strong scavengers of reactive oxygen and nitrogen species

“…For instance, a larger concentration of lipid peroxidation markers is detected in LDL samples of patients suffering from atherosclerosis rheumatic arthritis, after shock, or bacterial infection. 38 Noticeably, some of the tested 2-styrylchromones have previously shown to prevent tert-butylhydroperoxide (t-BHP) induced lipid peroxidation in rat hepatocytes. 8 Additionally, the compounds studied by Fernandes and coworkers reduced the prooxidant hepatotoxicity by preventing the depletion of reduced glutathione (GSH) and cell death.…”

“…The ROO Å scavenging activity was measured by monitoring the fluorescence decay resulting from ROO Å -induced oxidation of fluorescein and expressed as the 'Oxygen Radical Absorbance Capacity' (ORAC), according to a previously described method. 38 ROO Å was generated by thermodecomposition of AAPH. Reaction mixtures in the sample wells contained the following reagents at the indicated final concentrations (in a final volume of 200 lL): fluorescein (61 nM), the tested compounds (0.1-0.8 lM), dissolved in acetone and subsequently diluted in 75 mM phosphate buffer, pH 7.4, and AAPH (19 mM).…”

Anti-inflammatory potential of 2-styrylchromones regarding their interference with arachidonic acid metabolic pathways

“…Importantly, it has been demonstrated that the antioxidant effect of many NSAIDs may contribute to their therapeutic effectiveness [30,[49][50][51]. Thus, the strong antioxidant effects of 2-SC will certainly contribute to their anti-inflammatory efficacy in vivo.…”

Antioxidant Activity of gossypitrin isolated from the petals of Talipariti elatum (Sw.) Fryxell (Malvaceae) in Cuba

“…scavenging activity was measured by monitoring the decay in fluorescence due to the oxidation of fluorescein, accordingly to a described procedure known by oxygen radical absorbance capacity (ORAC) assay (Fernandes et al, 2004.). ROO .…”