In vitro seed germination and acclimatization of Encyclia cordigera (Kunth) Dressler

Baltazar-Bernal, O.; Cruz-Martínez, V.M. De la; Ramírez-Mosqueda, Marco A.; Zavala-Ruiz, J.

doi:10.1016/j.sajb.2022.10.031

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…VW and KC have more potassium phosphate monobasic content though the composition of potassium nitrate is lower than in MS medium [46]. MS and KC media have been extensively used for efficient seed germination and in vitro propagation of several orchids [47][48][49][50]. VC medium, on the other hand, has been mainly utilized for the proliferation of protocorm-like bodies, which helped in the orchid clonal multiplication on a large scale [51].…”

Section: Orchid Micropropagation Using Semi-solid Mediamentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

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Section: Orchid Micropropagation Using Semi-solid Mediamentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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“…Successful tissue culture plant propagation relies on explant sources, media composition, and precise application of plant growth regulators. Another critical factor is the effectiveness of the acclimatization process in the greenhouse, where plantlets adapt from in vitro culture to their natural environment, ensuring their successful establishment in the field [6]. Each plantlet's successful acclimatization in the greenhouse depended on suitable growing media and environmental conditions [7].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Growth Conditions for Green Grass Jelly (Cyclea barbata Miers) Derived in Vitro Culture: Greenhouse Acclimatization and Field Growth

Heryanto,

Fatimah Syahid

2023

E3S Web of Conf.

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Cyclea barbata Miers, a species within the Menispermaceae family, holds significance in medicinal and beverage applications. In vitro propagation is a common practice for conserving this plant; however, a comprehensive investigation of its growth characteristics within controlled greenhouse and natural field environments is essential. This study aims to determine the optimal planting media for acclimatization and the subsequent growth of Cyclea barbata Miers propagated from in vitro cultures. The experimental design encompasses two phases: 1) greenhouse acclimatization and 2) field growth. Various planting media, including combinations of sterile soil, manure, and husk charcoal, were rigorously evaluated during the acclimatization phase. Following the acclimatization period, the plants were transferred to the field. Multiple parameters were assessed: plant growth percentage, branch and node counts, leaf dimensions (length and width), and leaf wet and dry weights. Our findings reveal that a planting medium comprising sterile soil, manure, and husk charcoal provided the best performance growth outcomes during acclimatization. Furthermore, we observed that plant age exerts a discernible influence on the growth dynamics of Cyclea barbata Miers.

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In vitro seed germination and acclimatization of Encyclia cordigera (Kunth) Dressler

Cited by 2 publications

References 21 publications

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Optimizing Growth Conditions for Green Grass Jelly (Cyclea barbata Miers) Derived in Vitro Culture: Greenhouse Acclimatization and Field Growth

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