In vitro Seed Germination and Callus Induction of Ferula ferulaeoides (Steud.) Korov. (Apiaceae)

Suran, Dagdan; Bolor, Tumendalai; Gun-Aajav, Bayarmaa

doi:10.22353/mjbs.2016.14.07

Cited by 5 publications

(2 citation statements)

References 5 publications

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“…In vitro propagated seedlings are regularly used as an alternative source of vegetative material to optimize callus generation [ 54 , 55 , 56 ]. Oregano seeds were cultured in MS (Murashige and Skoog) media and aseptic seedlings were grown to obtain leaf explants for callus induction.…”

Section: Resultsmentioning

confidence: 99%

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Kakalis

Tsekouras

Mavrikou

et al. 2023

Plants

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Oregano (Origanum vulgare, Lamiaceae plant family) is a well-known aromatic herb with great commercial value, thoroughly utilized by food and pharmaceutical industries. The present work regards the comparative assessment of in vitro propagated and commercially available oregano tissue natural products. This study includes their secondary metabolites’ biosynthesis, antioxidant properties, and anticancer activities. The optimization of callus induction from derived oregano leaf explants and excessive oxidative browning was performed using various plant growth regulators, light conditions, and antioxidant compounds. The determination of oregano callus volatiles against the respective molecules in maternal herbal material was performed using gas chromatography–mass spectrometry (GC/MS) analysis. In total, the presence of twenty-seven phytochemicals was revealed in both leaf and callus extracts, from which thirteen molecules were biosynthesized in both tissues studied, seven compounds were present only in callus extracts, and seven metabolites only in leaf extracts. Carvacrol and sabinene hydrate were the prevailing volatiles in all tissues exploited, along with alkanes octacosane and triacontane and the trimethylsilyl (TMS) derivative of carvacrol that were detected in significant amounts only in callus extracts. The MTT assay was employed to assess the in vitro cytotoxic properties of oregano extracts against the epithelial human breast cancer MDA-MB-231 and the human neuroblastoma SK-N-SH cell lines. The extracts displayed concentration and time-dependent responses in cell proliferation rates.

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Section: Resultsmentioning

confidence: 99%

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Kakalis

Tsekouras

Mavrikou

et al. 2023

Plants

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“…Korov. Apiaceae (Suran et al 2016). The authors obtained white friable calli from cotyledon explants on the same culture medium composition.…”

Section: Morphological Features Of Callusmentioning

confidence: 99%

Antioxidant activities in Pimpinella anisum L. callus responding to PEG-6000-induced water stress under light (12h-photoperiod) and dark conditions

Lamara

Boucelha

Djebbar

et al. 2022

Preprint

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Water stress is one constraint of the development potential of many plant species under in vitro tissue culture. The present study aims to assess the responses of anise callus tissues to two concentrations (5% and 7.5%) of PEG-6000 under darkness and light (12H-photoperiod) for 30 days. The exposure of calli to water stress recorded morphological and physiological variations in fresh weight and water content in the callus decreased with increasing dry weight. Malondialdehyde content increased progressively with concentrations used with higher values under dark than in light. The activity of antioxidant enzymes (SOD, CAT, APX, and GPOX) was affected by PEG-6000 concentrations and culture conditions. APX and CAT increased gradually with 5% and 7.5% of PEG-6000 with higher values in light, but SOD enzyme reacted differently with a slight increase with 5% in light and higher ones under dark with 7.5%. Additionally, GPOX activity decreased with both concentrations independently of light or darkness. The results revealed that anise calli under drought stress with 7.5% PEG-6000 were more resistant to the lack of water in our experimental conditions.

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Antioxidant activities in Pimpinella anisum L. callus responding to PEG-6000-induced water stress under light (12 h-photoperiod) and dark conditions

Lamara¹,

Boucelha²,

Djebbar³

et al. 2023

Plant Cell Tiss Organ Cult

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In vitro Seed Germination and Callus Induction of Ferula ferulaeoides (Steud.) Korov. (Apiaceae)

Cited by 5 publications

References 5 publications

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

Antioxidant activities in Pimpinella anisum L. callus responding to PEG-6000-induced water stress under light (12h-photoperiod) and dark conditions

Antioxidant activities in Pimpinella anisum L. callus responding to PEG-6000-induced water stress under light (12 h-photoperiod) and dark conditions

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