In vitro seed germination, proliferation, and ISSR marker-based clonal fidelity analysis of Shorea tumbuggaia Roxb.: an endangered and high trade medicinal tree of Eastern Ghats

Shukla, Shastri Prasad; Sharma, Abhishek

doi:10.1007/s11627-017-9818-5

Cited by 18 publications

(4 citation statements)

References 18 publications

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“…BAP promoted multiple shoot formation, whereas cell elongation was limited. BAP has been used to induce budbreak and multiple shoot formation in several woody plant species, such as Balanites aegytiaca (Anis et al, 2010;Khamis et al, 2016), Melia azedarach (Husain and Anis, 2009), Salix tetrasperma (Khan et al, 2011), and Shorea tumbuggaia (Shukla and Sharma, 2017).…”

Section: Resultsmentioning

confidence: 99%

Micropropagation and Effect of Phloroglucinol on Rooting of Diospyros crassiflora Hiern

Tchouga¹,

Deblauwe²,

Djabou³

et al. 2020

horts

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The jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the long time required for rooting formation of cuttings are limiting its propagation. This study aims to develop a successful protocol for ebony micropropagation. In vitro culture of nodal segments from seedlings was performed in half-strength Murashige and Skoog (MS) medium supplemented with either zeatin (0.0, 2.3, 4.6, 9.1, 13.7, 18.2, 22.8, and 27.4 µm) or 6-benzylaminopurine (BAP) (0.0, 2.2, 4.4, 8.8, 13.3, 17.8, 22.2, and 26.6 µm). After 12 weeks, all media allowed shoot budbreak. Shoots displaying the greatest budbreak were observed with 22.8 µm zeatin and 22.2 µm BAP. The shoots were then transferred to a medium supplemented with indole-3-butyric acid (IBA) and phloroglucinol (PG) at different concentrations for root induction. Root induction was observed on the shoots initially induced in the medium with BAP, but not in those grown in the medium with zeatin. In half-strength MS supplemented with 396.5 µm PG plus 14.2 µm IBA, the formation of a single tap root was observed on 79% of shoots, with an average root length of 2.8 ± 0.18 cm. Rooted plants were successfully acclimatized to the greenhouse, with a 50% survival rate. This is the first report on Diospyros crassiflora micropropagation, which paves the way for rapid ebony multiplication to respond to needed conservation efforts.

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Section: Resultsmentioning

confidence: 99%

Micropropagation and Effect of Phloroglucinol on Rooting of Diospyros crassiflora Hiern

Tchouga¹,

Deblauwe²,

Djabou³

et al. 2020

horts

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“…In the last decades, plant tissue cultures have been instrumental in the rapid propagation and ex situ conservation of orchids, using different methods and explants including flower stalks, shoot tip nodes, stem bids, root tips, and rhizome segments [66]. The composi-tion of culture media plays a major role in in vitro seed germination and micropropagation using different explants [67,68]. For example, Murashige and Skoog (MS) medium enhances germination in the orchid Geodorum densiflorum, whereas Knudson C medium [69] is more suitable for Paphiopedilum seeds compared to other orchid-specific culture media [70].…”

Section: In Vitro Orchid Propagation In Plant Tissue Culturementioning

confidence: 99%

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Tiwari,

Sharma,

Bose

et al. 2024

Horticulturae

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Orchids constitute the largest and most diverse group of flowering plants and are classified in the family Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plant, the cultivation of orchids on a commercial level is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has successfully generated employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably bridged the knowledge gaps in areas of orchid classification, phytochemistry, and cultivation strategies. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, and their global marketing as cut flowers and artificially propagated plants. Orchids constitute the first floriculture crops that revolutionized the orchid industry; however, they also hold several challenges in the natural propagation and conservation of several species that are on the verge of extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming for conservation and legal commercial goals. This thematic review is one-of-a-kind in providing comprehensive insights into the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming times. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach to the conservation and commercialization of orchids.

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“…Micropropagation is not only a vivid method of ex-situ conservation but also provides the cultivation of endangered as well as commercially important plant species (Shukla and Sharma, 2017). Orchidaceae is one of the largest families of owering plants that have higher market demand that is ful lled either from their natural habitat or with high scale breeding, however, still a gap remains in their demand and supply, including the availability of rare and endangered orchids.…”

Section: In Vitro Orchid Propagation In Tissue Culturementioning

confidence: 99%

“…In the last several decades plant tissue culture techniques have been employed for rapid propagation and ex-situ conservation of orchids, using different methods and explants including ower stalk, shoot tip nodes, stems bids, root tips, and rhizome segments (Pant 2013;Reddy et al 2021). The composition of in vitro micropropagation media plays a major role in the in vitro seed germination and micropropagation using other explants (Shekarriz et al, 2014;Shukla and Sharma, 2017;Dwivedi et al, 2020). For example, MS media (Murashige and Skoog, 1962) promotes higher germination in orchid Geodorum densi orum, whereas Knudson C media (Knudson, 1946) was found more suitable for Paphiopedilum seeds compared to other orchid speci c culture media (Long et al, 2010;Muthukrishnan et al, 2013).…”

Section: In Vitro Orchid Propagation In Tissue Culturementioning

confidence: 99%

Biotechnological interventions in Orchids: Recent updates, Translational success, and Commercial outcomes

Tiwari

Sharma

Bose

et al. 2022

Preprint

Self Cite

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Orchids constitute the largest and most diverse group of flowering plants and are classified in Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plants, the cultivation of orchids on a commercial level, is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has been successful in generating employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably improved the knowledge in areas of orchid biology, classification, phytochemistry, and cultivation strategies, among other areas. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, witnessed as cultivation of different varieties and globally marketed as a cut flower and artificially propagated plants. The orchids constitute the first floriculture crops, which revolutionized the orchid industry, however, it also withholds several challenges in natural propagation, several species facing extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming towards conservation and legal commercial goals. This thematic review is one of a kind in providing comprehensive insights on the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming time. Together with traditional breeding approaches and plant tissue culture, advances in biotechnological interventions continues to make substantial progress in the development of exotic varieties, with multi-faceted attributes. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach for the conservation and commercialization of orchids.

show abstract

In vitro seed germination, proliferation, and ISSR marker-based clonal fidelity analysis of Shorea tumbuggaia Roxb.: an endangered and high trade medicinal tree of Eastern Ghats

Cited by 18 publications

References 18 publications

Micropropagation and Effect of Phloroglucinol on Rooting of Diospyros crassiflora Hiern

Micropropagation and Effect of Phloroglucinol on Rooting of Diospyros crassiflora Hiern

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Biotechnological interventions in Orchids: Recent updates, Translational success, and Commercial outcomes

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