In vitro selection and field responses of somaclonal variant plants of rice cv PR113 for drought tolerance

Verma, Dipti; Ansari, Mohammad Wahid; Agrawal, Ganesh Kumar; Rakwal, Randeep; Shukla, Alok; Tuteja, Narendra

doi:10.4161/psb.23519

Cited by 20 publications

(16 citation statements)

References 18 publications

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“…O PEG tem sido utilizado para estudos de restrição hídrica envolvendo a avaliação da germinação e seleção de genótipos tolerantes à salinidade e/ou seca em diferentes culturas (Hamayoun et al, 2011;Boyd & Hughes, 2011;Stanton et al, 2012;Hassanpanah, 2010;Hassanein, 2010;Soliman & Hendawy, 2013;Verma et al, 2013). Na literatura, o PEG surge para compor o meio de cultura descrito na forma de concentração (p/v) ou sob o efeito de potencial osmótico (MPa).…”

Section: Introductionunclassified

Influência do potencial hídrico induzido por polietilenoglicol in vitro na morfologia do trigo

Furlan

Saatkamp

Wiest

et al. 2014

Agraria

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RESUMOO desenvolvimento de uma planta depende de vários fatores ambientais que determinam a sua produtividade. O déficit hídrico é um dos principais fatores limitante na agricultura. O objetivo deste trabalho foi estabelecer um protocolo de avaliação do efeito do estresse hídrico em plântulas de trigo cultivadas in vitro. Os tratamentos constituíram da simulação dos seguintes potenciais (-0,1; -0,2; -0.3 e -0,4 MPa) com o uso de PEG -6000 e da testemunha. O delineamento experimental foi inteiramente casualizado com 5 tratamentos e 5 repetições. Foram avaliados como parâmetros fisiológicos e morfológicos: teor relativo de água (TRA), índice de estabilidade de membrana (IEM) e morfologia microscópica da raiz. Como resultados obtidos foi observado que o potencial osmótico de -0,3 MPa (PEG 6000 151,41g L -1 ) e o período de 5 dias propiciaram efeitos similares ao estresse hídrico em vaso, sendo este osmoregulador indicado para simular este estresse abiótico em condições laboratoriais.Palavras-chave: estresse hídrico, potencial osmótico, sistema radicular, Triticum aestivum L. In vitro influence of water potential induced by polyethylene glycol on the morphophysiology of wheat ABSTRACTPlant´s growing depends on several environmental factors that determine their productivity. Drought is a limiting factor for crop production. The aim of this study was the establishment of a protocol for evaluating in vitro, the water stress effect on wheat seedlings. Treatments consisted of simulating the following water potentials (-0.1; -0.2; -0.3 and -0.4 MPa) using PEG -6000 and the control. The experimental design was completely randomized with five treatments and five repetitions. It was evaluated morphological and physiological parameters such as: relative water content (RWC), membrane stability index (MEI) and the roots morphology by microscopy. As results, we observed that -0.3 MPa (PEG 6000 151.41 g L -1) osmotic potential and the 5 days period provided similar effects of water shortage as that one occurring at green house conditions, which is suitable for simulating this abiotic stress at laboratory conditions.

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Section: Introductionunclassified

Influência do potencial hídrico induzido por polietilenoglicol in vitro na morfologia do trigo

Furlan

Saatkamp

Wiest

et al. 2014

Agraria

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“…However, several interesting somaclonal variants have been reported in other crops. For instance, rice somaclones with increased drought tolerance have been reported [20] under field conditions. Somaclonal variants of wheat with increased drought tolerance than their donor parents (non-tissue cultured plants) have been identified in pots under greenhouse [25].…”

Section: Evaluation Of Somaclones (R1 Generation) and Their Respectivmentioning

confidence: 99%

“…During plant tissue culture, genetic variability, i.e., somaclonal variation may occur and the variation has an alternative tool for obtaining better performed plants regardless of important morphological and agronomical traits with increased tolerance to moisture stress. Somaclonal variations, for drought tolerance have been reported in many cereal crops, such as durum wheat [19], rice [20], sorghum [21] and peanut [18]. Rahayu [18] in peanut and Verma [20] in rice found drought tolerant somaclonal variants with better yield than the mother plants (non-tissue cultured plants).…”

Section: Advances In Crop Science and Technologymentioning

confidence: 99%

“…Somaclonal variations, for drought tolerance have been reported in many cereal crops, such as durum wheat [19], rice [20], sorghum [21] and peanut [18]. Rahayu [18] in peanut and Verma [20] in rice found drought tolerant somaclonal variants with better yield than the mother plants (non-tissue cultured plants). To date, little investment in biotechnology has been applied to tef.…”

Section: Advances In Crop Science and Technologymentioning

confidence: 99%

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Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Ferede¹,

Mekbib²,

Assefa³

et al. 2018

Adv Crop Sci Tech

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Breeding for moisture stress tolerance by conventional methods is not satisfactory in view of current demand to increase crop productivity. Inducing somaclonal variation is a potential technique to obtain stress tolerant variants. The present study was conducted to evaluate the performance of tef somaclones (R1 generation) and their respective parental genotypes for drought tolerance. The materials used in this experiment were obtained from the R0 generation of previous in vitro culture experiment. Twelve seed derived somaclones; Melko 0, Melko 0.5, Melko 1, Melko 1.5, Gemechis 0, Gemechis 0.5, Gemechis 1, Gemechis 1.5, Pop12S20, Pop12S20.5, Pop12S21 and Pop12S21.5 and their respective parental genotypes (Melko, Gemechis and Pop12S2) were used under two set of experiments (i.e., under moisture stress and non-stress conditions) in pots under green house. The experiments were laid out in completely randomized design with three replications. Analysis of variance revealed that somaclones (R1 generation) and their parental genotypes showed highly significant (P ≤ 0.01) difference in all the measured traits under both moisture regimes. Comparisons were made between the somaclones and their parents under moisture stress and non-stress conditions for the measured traits. Under moisture stress condition superiority of the somaclones was observed except for number of spikelet/panicle and total number of tiller/plant. On the other hand, under non-stress condition better performance was observed from parental genotypes for number of spikelet/ panicle, panicle weight, panicle seed weight, plant weight, plant seed weight and grain yield. The results showed that all the somaclones and their parental genotypes were negatively affected by moisture stress for all studied parameters. However, somaclones were less affected by moisture stress as compared to the parental genotypes. Hence, in vitro culture induced somaclonal variation can effectively be used for selection and improvement of drought tolerant tef genotypes.

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“…Tissue culture technique has emerged as a feasible and cost-effective alternative tool for developing stress-tolerant plants in recent years. This technique can operate under controlled conditions with minimal environmental interaction, limited space and time can complement field selection, and has the potential for developing of stress-tolerant varieties using a low cost laboratory set up (Sakhanokho, Kelley, 2009;Verma et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Cold acclimation efficiency of different Prunus and Fragaria species and cultivars in vitro

Rugienius

Šnipaitienė

Stanienė

et al. 2016

Zemdirbyste-Agriculture

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Winter hardiness is one of the main traits affecting survival, productivity and cultivar choice for important horticultural plants in a temperate climatic zone. Given the complexity of the trait and the often fluctuating winter weather, extensive field testing over many years would be required to gain reliable results. Testing for cold tolerance under controlled conditions to grade genotypes has been used in several plant breeding programs to identify superior genotypes of different species. The aim of the present study was to investigate cold tolerance of microshoots of different genotypes of the genera Prunus and Fragaria after 7, 14, 28 and 56 days of cold acclimation, by measuring ion leakage and evaluating the critical temperature (CT50) after freezing in vitro. Genotypes of Rosacea family demonstrated distinct patterns of CT50 change during cold acclimation. Cold acclimation for 56 days decreased the CT50 value by 1.3-2.0°C for Prunus microshots and by 0.8-2.1°C for Fragaria microshoots in comparison to nonacclimated plants. The results of cold acclimation and freezing treatments of Rosacea family plants in vitro show that for maximal cold hardiness, acclimation for 56 days or longer is required. Sour cherry (Prunus cerasus) cv. 'Orkolija', garden strawberry (Fragaria × ananassa) cv. 'Melody', Virginia strawberry (F. virginiana) and musk strawberry (F. moschata) were the most winterhardy after 56 days' cold acclimation. The data obtained in the study can be used to improve breeding and cryopreservation technologies of Rosacea plants, and lay the foundation for identification of genes responsible for efficient cold acclimation and low temperature tolerance.

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In vitro selection and field responses of somaclonal variant plants of rice cv PR113 for drought tolerance

Cited by 20 publications

References 18 publications

Influência do potencial hídrico induzido por polietilenoglicol in vitro na morfologia do trigo

Influência do potencial hídrico induzido por polietilenoglicol in vitro na morfologia do trigo

Evaluation of Tef (Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance

Cold acclimation efficiency of different Prunus and Fragaria species and cultivars in vitro

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