In vitro shoot proliferation and enhancement of rooting for the large-scale propagation of yellow bamboo (Bambusa vulgaris ‘Striata’)

Ramanayake, S.M.S.D.; Meemaduma, V. N.; Weerawardene, T. E.

doi:10.1016/j.scienta.2006.06.016

Cited by 50 publications

(43 citation statements)

References 15 publications

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“…These results were also supported by the earlier reports on micropropagation of bamboos, where BAP had been used extensively for shoot multiplication (Ramanayake et al, 2006;Yasodha et al, 2008;Ramanayake et al, 2008;Arya et al, 2008;Mudoi and Borthakur, 2009). TDZ has been proved an effective cytokinin for shoot proliferation in B. oldhamii (Lin et al, 2007); B. edulis (Lin and Chang, 1998); D. giganteous and B. vulgaris (Ramanayake et al, 2001 and.…”

Section: Resultssupporting

confidence: 85%

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Raju

Roy

2017

Jahangirnagar Univ. J. Biol. Sci.

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Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog's (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.

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Section: Resultssupporting

confidence: 85%

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Raju

Roy

2017

Jahangirnagar Univ. J. Biol. Sci.

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“…Axillary shoots of bamboo could not develop roots on the medium containing TDZ and IBA. It is concluded from different reports that pretreatment with TDZ inhibited root formation, however, incorporation of TDZ in rooting medium showed positive response (Ramanayake et al, 2006). In some cases, retarded development of TDZ-stimulated propagules may obtain from application of supera-optimal values of TDZ in the media or the presence of compound for longer time in cultured tissues (Mundhara and Rashid, 2006).…”

Section: Range Of Tdz Mediated Morphogenesismentioning

confidence: 96%

“…TDZ treatment causes rooting in the adult-derived tissues of Bambusa vulgaris (Ramanayake et al, 2006). reported the results of TDZ on the transport of root promoting auxin in many species.…”

Section: Range Of Tdz Mediated Morphogenesismentioning

confidence: 99%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

200

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Thidiazuron (TDZ) has gained a considerable attention during past decades due to its efficient role in plant cell and tissue culture. Wide array of physiological responses were observed in response to TDZapplication in different plant species. TDZ has shown both auxin and cytokinin like effects, although, chemically, it is totally different from commonly used auxins and cytokinins. A number of biological (physiological and biochemical) events in cells are induced or enhanced by TDZ, but the mode of action of TDZ is yet unknown. However, varieties of underlying mechanisms were revealed by reports showing how morphogenic events were induced by application of TDZ. Other reports showed that TDZ may modify endogenous plant growth regulators, either directly or indirectly and produce reactions in cell/tissue, necessary for its division/regeneration. Other possibilities include modification in cell membrane, energy levels, nutrient absorption, transport and assimilation, etc. In this review, recent advancements in TDZ application in plant sciences are discussed.

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“…In some other observations, the difficulty in rooting of shoots associated with the presence of TDZ in the shoot multiplication medium has been reported (Cruz de Carvalho et al 2000;Kozak 2010). On the other hand, Ramanayake et al (2006) found that rooting in a species of Bambusa vulgaris was increased if shoots were pretreated with TDZ before transferring to the rooting medium.…”

Section: Rooting Of Shootsmentioning

confidence: 99%

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

2014

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The effects of thidiazuron (TDZ) pretreatment of shoot tips on Harpagophytum procumbens shoot proliferation and successive stages of micropropagation, i.e. rooting of regenerated shoots and acclimatization of plantlets to ex vitro conditions, were described in the present study. The best response in terms of shoot proliferation (about seven shoots/explant) and shoot length (3.2 ± 0.4 cm) was obtained when explants pretreated with 25 lmol L -1 TDZ for 6 h were cultured on Schenk and Hildebrandt medium containing indole-3-acetic acid (IAA) (0.57 lmol L -1 ) and 6-benzylaminopurine (BAP) (8 lmol L -1 ). Under these conditions, a 330 % increase in shoot multiplication over TDZ non-pretreatment culture was achieved and TDZ pretreatment shoots were longer compared to those in control culture (2.6 ± 0.3 cm). The TDZ pretreatment did not affect the percentage of rooted shoots, length of roots and number of roots formed per shoot. The rooted plantlets were transplanted from in vitro to pots with soil and grown during 1 year in the greenhouse. The hardening process was difficult and time-consuming. We found that the plants developed from the TDZ pretreated culture were superior to plants from non-pretreated culture in terms of survival rate and morphological features, such as shoot length, leaf size, flowering and earlier root tuberisation. Random amplified polymorphic DNA and inter-simple sequence repeat analyses of pretreatment with TDZ plants showed genetic similarity to non-pretreatment plants. We conclude that applying the strategy of initial explant pretreatment with TDZ may be valuable for the improvement in H. procumbens in vitro propagation.

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In vitro shoot proliferation and enhancement of rooting for the large-scale propagation of yellow bamboo (Bambusa vulgaris ‘Striata’)

Cited by 50 publications

References 15 publications

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

Thidiazuron: A multi-dimensional plant growth regulator

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

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