In vitro shoot regeneration from cotyledons of immature ovules ofImpatiens platypetala Lindl.

Han, Kyungchul

doi:10.1007/bf02632138

Cited by 5 publications

(2 citation statements)

References 25 publications

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“…Published tissue culture protocols for the regeneration of plants from different type of explants of Impatiens spp. are available (3,8) but these proved unsatisfactory when applied to the cultivars used in this study. to develop improved methods of plant regeneration of I. wallerana the effect of two urea-type cytokinins, (cPPU) and thidiazuron (tDZ) was investigated.…”

Section: Introductionmentioning

confidence: 99%

Effect of Urea—Type Citokinins on Axillary Shoots Regeneration ofImpatiens WallerianaL.

Subotić¹,

Jevremović²,

Cingel³

et al. 2008

Biotechnology & Biotechnological Equipment

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Section: Introductionmentioning

confidence: 99%

Effect of Urea—Type Citokinins on Axillary Shoots Regeneration ofImpatiens WallerianaL.

Subotić¹,

Jevremović²,

Cingel³

et al. 2008

Biotechnology & Biotechnological Equipment

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“…There are few reports in the literature dealing with Impatiens tissue culture and transformation. These include micropropagation [ 6 - 9 ], in vitro germination of immature ovules [ 10 ], growth of cotyledon sections [ 11 ], callus culture [ 12 , 13 ] and embryo and ovule culture [ 14 , 15 ]. The majority of these publications reported callus induction from explants using New Guinea, Java Impatiens and Impatiens platypetala , with no de novo plant regeneration.…”

Section: Introductionmentioning

confidence: 99%

Development of Efficient Plant Regeneration and Transformation System for Impatiens Using Agrobacterium tumefaciens and Multiple Bud Cultures as Explants

et al. 2010

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BackgroundImpatiens (Impatiens walleriana) is a top selling floriculture crop. The potential for genetic transformation of Impatiens to introduce novel flower colors or virus resistance has been limited by its general recalcitrance to tissue culture and transformation manipulations. We have established a regeneration and transformation system for Impatiens that provides new alternatives to genetic improvement of this crop.ResultsIn a first step towards the development of transgenic INSV-resistant Impatiens, we developed an efficient plant regeneration system using hypocotyl segments containing cotyledonary nodes as explants. With this regeneration system, 80% of explants produced an average of 32.3 elongated shoots per initial explant plated, with up to 167 elongated shoots produced per explant. Rooting efficiency was high, and 100% of shoots produced roots within 12 days under optimal conditions, allowing plant regeneration within approximately 8 weeks. Using this regeneration system, we developed an efficient Agrobacterium-mediated Impatiens transformation method using in vitro multiple bud cultures as explants and a binary plasmid (pHB2892) bearing gfp and nptII genes. Transgenic Impatiens plants, with a frequency up to 58.9%, were obtained within 12 to 16 weeks from inoculation to transfer of transgenic plants to soil. Transgenic plants were confirmed by Southern blot, phenotypic assays and T1 segregation analysis. Transgene expression was observed in leaves, stems, roots, flowers, and fruit. The transgenic plants were fertile and phenotypically normal.ConclusionWe report the development of a simple and efficient Agrobacterium-mediated transformation system for Impatiens. To the best of our knowledge, there have been no reports of Agrobacterium-mediated transformation of Impatiens with experimental evidence of stable integration of T-DNA and of Agrobacterium-mediated transformation method for plants using in vitro maintained multiple bud cultures as explants. This transformation system has the advantages of 1) efficient, simple and rapid regeneration and transformation (with no need for sterilization or a greenhouse to grow stock plants), 2) flexibility (available all the time) for in vitro manipulation, 3) uniform and desirable green tissue explants for both nuclear and plastid transformation using Agrobacterium-mediated and biolistics methods, 4) no somaclonal variation and 5) resolution of necrosis of Agrobacterium-inoculated tissues.

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Efficient plant regeneration system for New Guinea Impatiens (Impatiens hawkeri W. Bull) CV. ‘Violet’ and ‘Scarlet Bronze Leaf’

Zhang

Wang

Zhou

et al. 2022

Plant Cell Tiss Organ Cult

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In vitro shoot regeneration from cotyledons of immature ovules ofImpatiens platypetala Lindl.

Cited by 5 publications

References 25 publications

Effect of Urea—Type Citokinins on Axillary Shoots Regeneration ofImpatiens WallerianaL.

Effect of Urea—Type Citokinins on Axillary Shoots Regeneration ofImpatiens WallerianaL.

Development of Efficient Plant Regeneration and Transformation System for Impatiens Using Agrobacterium tumefaciens and Multiple Bud Cultures as Explants

Efficient plant regeneration system for New Guinea Impatiens (Impatiens hawkeri W. Bull) CV. ‘Violet’ and ‘Scarlet Bronze Leaf’

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