In vitro Sterilization, Rooting and Acclimatization of Difficult-to-root Bougainvillea Cultivars

Kumari, Pratiksha; Swaroop, Kishan; Janakiram, T.; Singh, Shweta; Prasad, K.; Jain, Ritu; landscaping, Iari; Bhawan, New Delhi Icar Krishi Anusandhan

doi:10.23910/ijbsm/2016.7.3.1530

Cited by 4 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent investigations have shifted toward understanding the tissue culture variations among different Bougainvillea varieties, aiming to develop a more efficient system that lowers costs and enhances benefits. For instance, Kumari et al [22] conducted in vitro root culture and domestication studies on challengingto-root Bougainvillea varieties, Mahatma Gandhi and Refulgens. Jain et al [23] provided a protocol for the in vitro multiplication of the Bougainvillea cultivar Mahara.…”

Section: Introductionmentioning

confidence: 99%

An Efficient Method for the Propagation of Bougainvillea glabra ‘New River’ (Nyctaginaceae) from In Vitro Stem Segments

Lin,

Xu,

et al. 2024

Forests

View full text Add to dashboard Cite

Bougainvillea, an evergreen climbing shrub of the Nyctaginaceae family, holds significant ornamental, economic, and medicinal value. Bougainvillea glabra ‘New River’ is widely used in landscapes due to its strong adaptability to the environment, abundance of flowers, and frequent flowering. Traditionally, Bougainvillea glabra ‘New River’ cultivation has relied on methods such as cuttings or grafting, with limited research on in vitro tissue culture propagation. This study aimed to optimize the tissue culture system, exploring a combination of plant growth regulators (PGRs) for Bougainvillea regeneration from in vitro stem segments. The Murashige and Skoog (MS) medium supplemented with indole-3-butyric acid (IBA), 6-benzylaminopurine (6-BA), and 1-naphthlcetic acid (NAA) was employed. The optimal sterilization of Bougainvillea stem segments involved a 30 s treatment with 75% alcohol and 10 min with 1% NaClO. The synergistic effect of 0.1 mg·L−1 of NAA and 2.5 mg·L−1 of 6-BA maximized the shoot sprouting frequency, while 2.5 mg·L−1 of 6-BA and 0.1 mg·L−1 of NAA produced the maximum shoots. Furthermore, 1.5 mg·L−1 of IBA and 0.1 mg·L−1 of NAA induced the highest rooting levels. This work demonstrates the successful adaptation of a greenhouse environment to efficiently regenerate plants in vitro from stem segments. This approach allows for the mass production of Bougainvillea glabra ‘New River’.

show abstract

Section: Introductionmentioning

confidence: 99%

An Efficient Method for the Propagation of Bougainvillea glabra ‘New River’ (Nyctaginaceae) from In Vitro Stem Segments

Lin,

Xu,

et al. 2024

Forests

View full text Add to dashboard Cite

show abstract

Challenges and insights in the acclimatization step of micropropagated woody plants

Grzelak,

Pacholczak,

Nowakowska

2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Acclimatization is the last step of plants in vitro propagation while it comes to alter the environment conditions from tailor for plant requirements, sterile laboratory to external ex vitro conditions. Consequently, during acclimatization it may reach to plants mortality due to abiotic and biotic stresses occurring in external environment (high temperature, light intensity, low air relative humidity and the presence of microorganisms and pathogens in soil). Micropropagated plants develop alter organs on morphological, anatomical or biochemical levels which must be correct during acclimatization to improve adaptation of plants to ex vitro conditions. Therefore, there is a need to create acclimatization protocols to improve the effectiveness of this vegetative propagation method. So far there were formulated facilities as pre-acclimation, in vitro acclimatization, water cultures, CO2 enrichment, application of biostimulators and plant growth regulators. In following research we focused on describing previous achievements about the changes occurring in woody plants by micropropagation and summary of achievements in acclimatization of micropropagated woody plants. This article summary the current state of knowledge on acclimatization for various range of woody species by anatomical, physiological and biochemical levels. There are described ways to increase the survival of acclimatized plants and minimize acclimatization stress it also collects substances used so far to enhance plant growth during acclimatization.

show abstract

Morphological, Anatomical, Physiological and Biochemical Changes during Adventitious Roots Formation of Bougainvillea buttiana ‘Miss Manila’

et al. 2022

View full text Add to dashboard Cite

Bougainvillea, a widely used ornamental plant, is mainly propagated by cuttings and tissue culture. Still, large-scale production of Bougainvillea is often difficult because of rooting issues. Therefore, based on an early establishment of the regeneration system for tissue culture in Bougainvillea by our research team, we further studied its rooting mechanism. It was observed that the morphology and anatomical structure of Bougainvillea buttiana ‘Miss Manila’ contained endogenous hormones, such as indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellic acid (GA3), and zeatin-riboside (ZR), including peroxidase (POD), polyphenol oxidase (PPO), and IAA-oxidase (IAAO) activities. Following the culture, Days 0–15 consisted of the induction and initiation stages, while Days 15–25 included the expression stages. No latent root primordium was found in the Bougainvillea plantlet, which belonged to the induced rooting type. The root primordium was derived from callus cells generated by divisions of parenchyma cells in the basic tissues. It was found that the changes in the POD, PPO, and IAAO activities were closely related to the formation of adventitious roots (AR), in which the highest rooting values occurred during the transition from the initiation stage to the expression stage, whereas the endogenous IAA and ABA contents had negative and positive correlations during the induction, initiation, and expression stages.; The values of GA3 and ZR also peaked during the transition from the initiation to the expression stage. ZR and GA3 were found to promote adventitious root formation, while ABA inhibited it. The IAA/ABA, ABA/ GA3, and IAA/ZR ratios also shifted at the onset of the expression stage of AR, indicating these values were closely related to their occurrence. Overall, this study provides the basis for further research considering AR formation in Bougainvillea, and the propagation of various Bougainvillea varieties.

show abstract

In vitro Sterilization, Rooting and Acclimatization of Difficult-to-root Bougainvillea Cultivars

Cited by 4 publications

References 0 publications

An Efficient Method for the Propagation of Bougainvillea glabra ‘New River’ (Nyctaginaceae) from In Vitro Stem Segments

An Efficient Method for the Propagation of Bougainvillea glabra ‘New River’ (Nyctaginaceae) from In Vitro Stem Segments

Challenges and insights in the acclimatization step of micropropagated woody plants

Morphological, Anatomical, Physiological and Biochemical Changes during Adventitious Roots Formation of Bougainvillea buttiana ‘Miss Manila’

Contact Info

Product

Resources

About