In vitro studies of Annona muricata L. extract‐loaded electrospun scaffolds for localized treatment of breast cancer

Abstract

This paper presents in vitro studies of the sustained release of Annona muricata leaf extracts (AME) from hybrid electrospun fibers for breast cancer treatment.Electrospun hybrid scaffolds were fabricated from crude AME extracts, poly(lacticco-glycolic acid)/gelatin (PLGA/Ge) and pluronic F127. The physicochemical properties of the AME extract and scaffolds were studied. The antiproliferative effects of the scaffolds were also assessed on breast cancer (MCF-7 and MDA-MB-231) and non-tumorigenic breast (MCF10A) cell lines. Scanning electron microscope micrographs revealed a random network of micro-and submicron fibers. In vitro drug release profiles, governed by quasi-Fickian diffusion at pH 7.

Antitumor Potential of Annona muricata Linn. An Edible and Medicinal Plant in Mexico: In Vitro, In Vivo, and Toxicological Studies

“…Acetogenins have been identified as one of the major bioactive compounds of A. muricata with activity on several cancer cell lines [15,16]. The ethanol extracts obtained from aerial parts from A. muricata in combination with doxorubicin showed important cytotoxic activity on 4T1 cells and other cancer cell lines [15][16][17][18][19][20][21][22]. Although there is an extensive amount of information about the cytotoxic properties of A. muricata, no information is available regarding its anti-cancer potential in specifically breast cancer using the extracts of plants collected in different periods of the year, and regarding the location of the place of recollection and its influences on their pharmacological and toxic properties.…”

Tissue Engineering Scaffolds Loaded With a Variety of Plant Extracts: Novel Model in Breast Cancer Therapy

“…It is frequently treated using a trimodal approach that combines surgery, chemo, and radiotherapy. 3 , 9 …”

“… 39 , 66 They emphasize how effective drug encapsulation and regulated release can reduce systemic toxicity and the chance of tumor recurrence. 65 However, a complete understanding of the practical implications of the medicines is hampered by the lack of data demonstrating clinical outcomes or patient reactions following therapy. 39 In a test on mice, all mice survived the procedure and both methods and treatments were well received as the mice showed minimal weight changes.…”

“…Recent research has delved into the potential of creating targeted drug delivery methods for cancer, aiming to address some of the challenges associated with widespread chemotherapy. 3 …”

The use of medicinal plants for combating breast cancer: A comprehensive review

“…The anti-proliferative effect of the ethyl acetate extract of AM leaf was due to a larger quantity of cytotoxicity on breast tumor tissue, which was caused by the A. muricata extract (Hadisaputri et al 2021). Incubation of MCF-7 and MDA-MB-231 cells with scaffolds, including A. muricata leaf extract, inhibited their growth (Akpan et al 2021). Kariyil et al (2021) also demonstrated the lethal effect of A. muricata seeds extract by cellular membranes lysis and S-stage arrest.…”