In vitro Studies on Portulaca grandiflora Hook

Portulaca grandiflora Hook. and P. oleracea L.: Formation of Betalains and Unsaturated Fatty Acids

“…They were explanted on MS medium with 4 mg/l 2,4-D (Adachi 1970;Endress 1976) or 1 mgjl 2,4-D and a reduced sucrose content of 20 gjl (Konar 1978) as weIl as on the modified MS medium described above . High auxin concentrations were lowered to 2 mg/l for the cultivation of isolated primary calli.…”

“…High auxin concentrations were lowered to 2 mg/l for the cultivation of isolated primary calli. While the medium of White (1963), as weIl as that of Heller or Knudson, was unsuitable for callus induction (Konar 1978), it allowed subculture of the cells (Endress 1976). The medium ofNagata and Takebe (1971), as weIl as that ofSchenk and Hildebrandt (1972), caused high growth rates, but seemed to repress betacyanin formation (Endress 1976).…”

“…The medium ofNagata and Takebe (1971), as weIl as that ofSchenk and Hildebrandt (1972), caused high growth rates, but seemed to repress betacyanin formation (Endress 1976). Konar (1978) found excellent proliferation when 1 mgjl kinetin and 10 g/l coconut milk were added to modified MS medium without auxin.…”

“…Mostly they led to callus formation, but cell cultures derived from this plant material could either not be obtained or were not studied in detail (Konar 1978;Böhm et al 1991).…”

“…7. white suspension cultures (Konar 1978;Böhm et al 1991). An explanation of these phenomena is still missing.…”