In vitro studies on the existence of endothelial precursor cells in the subectodermal avascular region of quail wing buds

Seifert, Roswitha

doi:10.1007/s004410050183

Cited by 1 publication

(4 citation statements)

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“…In the present paper, we present evidence that local influences from the grafting site modify these findings. Moreover, we did not observe differences in the angiogenic capacity of the avascular zones of later stages which is in line with recent in vitro results (Seifert, 1994).…”

Section: Introductionsupporting

confidence: 93%

“…QH-1-positive cells in the periphery of the limb bud therefore can be regarded as angioblasts that are about to join the central plexus of vessels. An angiogenic capacity of avascular peripheral wing mesoderm was recently shown by in vitro studies of Seifert (1994). Our grafting experiments show these cells are capable of taking part in capillary formation.…”

Section: Discussion the Angiogenic Capacity Of The Limb Mesodermsupporting

confidence: 68%

“…After this stage, the avascular zone appeared to be depleted of angioblasts. This finding is suggestive of the idea that the QH-1-positive cells arose in the periphery of the limb mesoderm but is in contrast to the results of Seifert (1994) and the results presented in this study. Our immunohistochemical studies on QH-1 labelled cells in semithin sections showed isolated QH-1 positive cells in the periphery of the quail wing bud independently of the stage of the embryo, at least up to stage 25 HH.…”

Section: The Origin Of Qh-1-positive Cells In the Avascular Zonecontrasting

confidence: 50%

“…In vitro studies of Seifert (1994) show that independently of the method of isolation, the peripheral wing mesoderm always contains angioblasts. Angioblasts that are derived from the peripheral wing mesoderm from quail embryos stages 18-25 HH were found to grow and proliferate under in vitro conditions.…”

Section: The Influence Of the Grafting Sitementioning

confidence: 99%

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Blood vessel formation in the avian limb bud involves angioblastic and angiotrophic growth

Brand‐Saberi

Seifert

Grim³

et al. 1995

Developmental Dynamics

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The vasculature of the avian limb bud takes its origin from the intersomitic vessels as can be shown by ink perfusion of the embryo. While the primitive vessels form a central network in the early limb bud, an area of about 100 p m in width from the ectoderm inward remains free from lumenized vessels. However, this subectodermal avascular zone contains isolated angioblasts, which can be demonstrated by confocal laser scanning microscopy in connection with QH-1-staining. QH-1-positive cells from the avascular zone are capable of giving rise to endothelial cells when grafted ectopically into a "permissive" environment such as the dorso-lateral paraxial mesoderm. Several grafting sites are compared regarding their permissiveness for capillary formation. In order to investigate the origin of the QH-1-positive angioblasts we carried out injections of DiI-Ac-LDL, which is specifically taken up by endothelial cells and macrophages, and found the lumenized vessels and a few isolated cells in the peripheral limb mesoderm stained. In double-labelling studies combining DiI-Ac-LDL and QH-1, it can be shown that there exists a pool of isolated angioblasts that are only QH-l-positive, but have not incorporated DiI-Ac-LDL. In contrast to the lumenized vessels in the core of the limb bud, we found that angioblasts in the avascular zone do not proliferate, as shown by proliferation studies applying the BrdU-method to semithin sections in connection with QH-l-labelled parallel sections. We conclude that the vascularization of the avian limb bud is achieved by a combination of angiotrophic growth (sprouting of vessels) and angioblastic growth (recruitment of angioblasts from the limb mesoderm).0 1995 Wiley-Liss, Inc.

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