In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

Firdous, S.; Nawaz, M.; Ikram, Manzoor; Ahmed, Mushtaq

doi:10.1134/s1054660x12030048

Cited by 10 publications

(9 citation statements)

References 38 publications

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“…The use of PS is necessary to reduce cell viability. The increase in percentage viability might be accounted for enhanced cell division resulting from the accelerated mitochondrial activity on light irradiation (Firdous, Nawaz et al 2012).…”

Section: Resultsmentioning

confidence: 99%

In-vitro Evaluation of Anticancer Activity of Rhodamine-640 perchlorate on Rhabdomyosarcoma cell line

Yaseen

Khurshed

Ullah

et al. 2022

Preprint

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Photodynamic therapy (PDT) is a well-recognized treatment modality for oncological and non-oncological diseases. In this current research Rhodamine (Rh-640 perchlorate), a second generation photosensitizer, mediated photodynamic effects are evaluated. Different controlling parameters i.e., optimum intracellular drug concentration, light dose response on lower scale are investigated on in-vitro human Rhabdomyosarcoma cancer cell line. Diode laser 630 nm was used as a light source and three treatment arms were selected for photodynamic efficacy evaluation. An optimum incubation time of 3 hours was found for Rh-640 mediated exposure of RD culture. Effective intracellular drug accumulation was optimized for culture when administered with different concentrations (5, 10, 20, 30, 50, 70, and 100) µM. Photodynamic action of Rh-640 at different light doses (2, 5, 15) J/cm2 was evaluated. It was found that post incubation with optimized parameters Rhodamine 640 mediated photodynamic therapy showed light dose dependent cytotoxicity i.e., (85% (2 J/cm2), 80%( 5 J/cm2), 69%( 15 J/cm2). Current research work suggest the potential photodynamic action of Rh-640 and may be evaluated its efficacy on other cancer cell lines.

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Section: Resultsmentioning

confidence: 99%

In-vitro Evaluation of Anticancer Activity of Rhodamine-640 perchlorate on Rhabdomyosarcoma cell line

Yaseen

Khurshed

Ullah

et al. 2022

Preprint

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“…A cytotoxic study of 5-ALA-mediated PDT on cancer cells was performed as a proof of concept of CELL-LEF. As a positive control for this experiment, we used a hepatocellular carcinoma cell line (HepG2) known to be highly sensitive to 5-ALA-mediated PDT [73][74][75][76]. We also used a human glioblastoma cell line (U87), for which studies are inconclusive, due to the unknown optimal parameters for high-efficacy PDT [77].…”

Section: In Vitro Cytotoxic Pdt Study Using Cell-lefmentioning

confidence: 99%

A Warp-Knitted Light-Emitting Fabric-Based Device for In Vitro Photodynamic Therapy: Description, Characterization, and Application on Human Cancer Cell Lines

Thecua

Ziane

Grolez

et al. 2021

Cancers

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Photodynamic therapy (PDT) appears to be a promising strategy in biomedical applications. However, the complexity of its parameters prevents wide acceptance. This work presents and characterizes a novel optical device based on knitted light-emitting fabrics and dedicated to in vitro PDT involving low irradiance over a long illumination period. Technical characterization of this device, called CELL-LEF, is performed. A cytotoxic study of 5-ALA-mediated PDT on human cancer cell lines is provided as a proof of concept. The target of delivering an irradiance of 1 mW/cm2 over 750 cm2 is achieved (mean: 0.99 mW/cm2; standard deviation: 0.13 mW/cm2). The device can maintain a stable temperature with the mean thermal distribution of 35.1 °C (min: 30.7 °C; max: 38.4 °C). In vitro outcomes show that 5-ALA PDT using CELL-LEF consistently and effectively induced a decrease in tumor cell viability: Almost all the HepG2 cells died after 80 min of illumination, while less than 60% of U87 cell viability remained. CELL-LEF is suitable for in vitro PDT involving low irradiance over a long illumination period.

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“…The presented techniques can be used for new light-based diagnostic apparatus for the quantities analysis of dengue patients. In our previous research findings, we have characterized tissues and biomaterials for optical imaging to diagnose diseases and develop optical detection equipments [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53].…”

Section: Confocal Microscopic Analysismentioning

confidence: 99%

Optical Diagnostic of Dengue Virus Infected Human Blood using Raman, Polarimetric and Fluorescence Spectroscopy

Firdous¹,

Anwar²

2017

Dengue - Immunopathology and Control Strategies

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In this chapter, we present the optical diagnosis of normal and dengue viral-infected human blood using Raman, Polarimetric, Transmission, and Fluorescence Spectroscopic techniques. The possibility of using light in diagnosis and treating illness has been known for thousands of years. The properties of light and lasers provided many modern applications at home, in industry, and in the field of medicine. Laser use in the field of medicine is large and steadily growing. This growth is based on the versatility of laser light. Efficient and accurate diagnosis of dengue is of primary importance for clinical care. A range of laboratory diagnostic methods has been developed to support patient management and disease control. The choice of diagnostic method depends on the purpose for which the testing is done, the type of laboratory facilities and technical expertise available, costs, and the time of sample collection. The dengue viral infection is mostly diagnosed through laboratory tests; these tests include detection of the virus, virus antigen, anti-dengue virus antibody, complement fixation test, neutralization tests, and detection of virus nucleic acid. As dengue infection most rapidly increases in different regions, early diagnostic confirmation of dengue infection in patients allows for timely clinical intervention, etiological investigation, and disease control. Hence, diagnosis of dengue disease during the acute phase should be a priority and is a public health concern. Lasers and optics have many applications in medical sciences; diagnosis and treatment of diseases with lasers and light are latest and noninvasive techniques. Development of light-based apparatus has evolved into tools for improved diagnosis and treatment modalities in medical sciences. The methods of the laser spectroscopy make it possible to obtain direct information regarding the structure and dynamics of the functional groups of biomolecules. Development of new light sources, optics, and diode laser of different wavelengths makes them attractive for spectroscopy of biological molecules. In our study, more than 600 dengue viral-infected blood or blood sera samples and 25 non-dengue healthy blood samples were analyzed using four different optical methodologies. In the first study, Raman spectrum peaks for normal samples observed at 1527, 1170, and 1021 cm −1 show the presence of different biological materials, including lipids, carbohydrates, skeletal CC stretch of acyl chains, and

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In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

Cited by 10 publications

References 38 publications

In-vitro Evaluation of Anticancer Activity of Rhodamine-640 perchlorate on Rhabdomyosarcoma cell line

In-vitro Evaluation of Anticancer Activity of Rhodamine-640 perchlorate on Rhabdomyosarcoma cell line

A Warp-Knitted Light-Emitting Fabric-Based Device for In Vitro Photodynamic Therapy: Description, Characterization, and Application on Human Cancer Cell Lines

Optical Diagnostic of Dengue Virus Infected Human Blood using Raman, Polarimetric and Fluorescence Spectroscopy

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