In vitro study of enhanced osteogenesis induced by HIF-1α-transduced bone marrow stem cells

Zou, Duohong; Han, Wei; Shang, You; Ye, D.; Wang, Liang; Wang, S.; Zhao, Jun; Zhang, W.; Jiang, Xinquan; Zhang, Xinquan; Huang, Yadong

doi:10.1111/j.1365-2184.2011.00747.x

Cited by 70 publications

(58 citation statements)

References 40 publications

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“…12,13 Previous studies have demonstrated that hypoxia-inducible factor (HIF) influences mesenchymal cells to differentiate along the osteoblast pathway, in part through activating osterix gene expression. 14,15 HIF is also an active nucleoprotein that enhances the production of corresponding proteins, thereby launching the process of angiogenesis. 16 It is therefore proposed that this agent could promote the DO process by simultaneously enhancing osteogenesis and angiogenesis.…”

mentioning

confidence: 99%

The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: an animal study

Jiang

Zhang

Fan

et al. 2016

International Journal of Oral and Maxillofacial Surgery

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mentioning

confidence: 99%

The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: an animal study

Jiang

Zhang

Fan

et al. 2016

International Journal of Oral and Maxillofacial Surgery

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“…The housekeeping gene, GAPDH, was used for normalization. 25,26 The specific primer sequences used in this study are listed in Table 1.…”

Section: Reverse Transcription and Real-time Pcr Assaymentioning

confidence: 99%

“…26,27 A semiquantitative analysis of alkaline phosphatase and calcium deposition was performed as previously described. 25 Alkaline phosphatase activity was determined by measuring the OD values for absorbance at 405 nm after incubation with p-nitrophenyl phosphate (Sigma) for 30 minutes at 37°C. For quantification of Alizarin Red S, the stained samples were desorbed using 10% cetylpyridinium chloride (Sigma), and the OD values for absorbance of the eluent were determined at 590 nm.…”

Section: Cell Adhesion Ability Assaymentioning

confidence: 99%

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Huang³

et al. 2013

IJN

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Background and methods:Various methods have been used to modify titanium implant surfaces with the aim of achieving better osseointegration. In this study, we fabricated a clustered nanorod structure on an acid-etched, microstructured titanium plate surface using hydrogen peroxide. We also evaluated biofunctionalization of the hybrid micro/nanorod topography on rat bone marrow mesenchymal stem cells. Scanning electron microscopy and x-ray diffraction were used to investigate the surface topography and phase composition of the modified titanium plate. Rat bone marrow mesenchymal stem cells were cultured and seeded on the plate. The adhesion ability of the cells was then assayed by cell counting at one, 4, and 24 hours after cell seeding, and expression of adhesion-related protein integrin β1 was detected by immunofluorescence. In addition, a polymerase chain reaction assay, alkaline phosphatase and Alizarin Red S staining assays, and osteopontin and osteocalcin immunofluorescence analyses were used to evaluate the osteogenic differentiation behavior of the cells. Results:The hybrid micro/nanoscale texture formed on the titanium surface enhanced the initial adhesion activity of the rat bone marrow mesenchymal stem cells. Importantly, the hierarchical structure promoted osteogenic differentiation of these cells. Conclusion: This study suggests that a hybrid micro/nanorod topography on a titanium surface fabricated by treatment with hydrogen peroxide followed by acid etching might facilitate osseointegration of a titanium implant in vivo.

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“…32,33 A semiquantitative analysis of alkaline phosphatase was performed as previously described. 34 Alkaline phosphatase activity was determined by measuring the optical density values for absorbance at 405 nm after incubation with p-nitrophenyl phosphate (Sigma-Aldrich, St Louis, MO, USA) for 30 minutes at 37°C. The intracellular total protein content was determined using the Micro BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA), and the alkaline phosphatase activity was normalized to the total protein content.…”

Section: Cell Morphology and Proliferationmentioning

confidence: 99%

Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function

Wang¹,

Li²,

Zhang³

et al. 2014

IJN

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As one of the important ions associated with bone osseointegration, magnesium was incorporated into a micro/nanostructured titanium surface using a magnesium plasma immersion ion-implantation method. Hierarchical hybrid micro/nanostructured titanium surfaces followed by magnesium ion implantation for 30 minutes (Mg30) and hierarchical hybrid micro/nanostructured titanium surfaces followed by magnesium ion implantation for 60 minutes (Mg60) were used as test groups. The surface morphology, chemical properties, and amount of magnesium ions released were evaluated by field-emission scanning electron microscopy, energy dispersive X-ray spectroscopy, field-emission transmission electron microscopy, and inductively coupled plasma-optical emission spectrometry. Rat bone marrow mesenchymal stem cells (rBMMSCs) were used to evaluate cell responses, including proliferation, spreading, and osteogenic differentiation on the surface of the material or in their medium extraction. Greater increases in the spreading and proliferation ability of rBMMSCs were observed on the surfaces of magnesium-implanted micro/nanostructures compared with the control plates. Furthermore, the osteocalcin ( OCN ), osteopontin ( OPN ), and alkaline phosphatase ( ALP ) genes were upregulated on both surfaces and in their medium extractions. The enhanced cell responses were correlated with increasing concentrations of magnesium ions, indicating that the osteoblastic differentiation of rBMMSCs was stimulated through the magnesium ion function. The magnesium ion-implanted micro/nanostructured titanium surfaces could enhance the proliferation, spreading, and osteogenic differentiation activity of rBMMSCs, suggesting they have potential application in improving bone-titanium integration.

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In vitro study of enhanced osteogenesis induced by HIF-1α-transduced bone marrow stem cells

Abstract: These results indicate that Lenti-HIF-1α can induce BMSC overexpression levels of angiogenic and osteogenic genes in vitro in the normoxic state. Further study will be focused on whether HIF-1α can also improve bone repair in vivo.

Cited by 70 publications

References 40 publications

The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: an animal study

The effects of hypoxia-inducible factor (HIF)-1α protein on bone regeneration during distraction osteogenesis: an animal study

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Magnesium ion implantation on a micro/nanostructured titanium surface promotes its bioactivity and osteogenic differentiation function

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