In Vitro Techniques to the Conservation and Plant Regeneration of Malanga (Colocasia esculenta L. Schott)

Abstract

<jats:p>Malanga (<jats:italic>Colocasia esculenta</jats:italic>) is a plant genetic resource that requires biotechnological strategies for conservation and propagation. One time-, labor-, and space-saving option is in vitro conservation and regeneration. The objective of this study was to develop a protocol for in vitro regeneration and conservation of germplasm of <jats:italic>C. esculenta</jats:italic> var. <jats:italic>criolla</jats:italic>. For conservation through minimal growth, we assessed several concentrations of Murashige and Skoog (MS) medium (one-third, one-half, and three-quarter strength), the growth retardant ancymidol (0, 1, 2, and 3 mg·L<jats:sup>−1</jats:sup>), and the osmoregulator polyethylene glycol (PEG-8000 mw) at different concentrations (0, 10, 20, and 30 g·L<jats:sup>−1</jats:sup>). For in vitro conservation, the percent survival, shoot number and length, and number of leaves and roots per explant were evaluated after 24 weeks. For in vitro regeneration, different concentrations of thidiazuron (TDZ: 0, 0.5, 1, 1.5, and 2 mg·L<jats:sup>−1</jats:sup>) and 6-benzylaminopurine (BAP; 0, 1, 2, 3, and 4 mg·L<jats:sup>−1</jats:sup>) were evaluated. After 4 weeks of cultivation, the percent response, shoot number, and number of leaves per explant were recorded. During in vitro conservation, it was noted that the treatment including 2 mg·L<jats:sup>−1</jats:sup> ancymidol resulted in a retarded development, without affecting the survival of the <jats:italic>C. esculenta</jats:italic> germplasm. With regard to shoot regeneration, 7.60 shoots per explant were obtained using 2 mg·L<jats:sup>−1</jats:sup> TDZ. Finally, 98% survival was achieved during the acclimatization process. This study will contribute to the establishment of genetic improvement programs through in vitro conservation and propagation of this valuable plant genetic resource.</jats:p>

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

“…[ 13 ] and apple ( Malus domestica ) [ 19 ]. Taro micropropagation has been carried out in semi-solid medium [ 20 ] and in a TIB [ 5 ]; however, other bioreactors need to be validated as an alternative for micropropagation. The aim of the present study was to compare the efficiency of the TIB, SETIS™, ebb-and-flow bioreactor, and semi-solid medium culture systems for in vitro multiplication of C. esculenta .…”

Kajian Konsentrasi Media dan Hormon untuk Konservasi Apel In Vitro

“…Keberhasilan konservasi dipengaruhi oleh interaksi penting antara factor fisika dan kimia dalam kultur in vitro (Rezende et al, 2018), sehingga dalam studi selanjutnya perlakuan yang dapat dicoba antara lain kombinasi suhu rendah dan perlakuan gelap (Benelli et al, 2012), perlakuan inhibitor etilen (Cha-um & Kirdmanee, 2007), asam absisat (Mona et al, 2016) atau dengan mencoba media dasar lainnya seperti WPM (Alvim et al, 2020) dan zat pengatur tumbuh lainnya (Mancilla-Álvarez et al, 2019;Pan et al, 2014;Montalvo-Peniche et al, 2007;Rida et al, 2015).…”