In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung

Abstract

Increasing natural ingredient awareness and utilization has created an increased demand for sources of natural medicinal ingredients, including sources of compound used to treat malaria. Streptomyces is a genus of prokaryote well recognized for its production of antibiotics and other pharmaceutically useful compound. This study aimed to assess the ability of unpurified fermentation metabolites to inhibit Plasmodium parasites. A strain of bacteria identified as Streptomyces hygroscopicus subsp. hygroscopicus strain i18 were isolated from pineapple fields in Lampung province, and was cultured and fermented on liquid synthetic Gause medium for 10 days. The supernatant was separated from the cells and extracted with ethyl acetate-methanol (1:1). Plasmodium falciparum 3D7 was used for antiplasmodial testing. Metabolites were tested qualitatively using a phytochemical approach. Saponins and triterpenoids were found to be present in the extract. Parasite inhibition as measured using probit analysis and yielded an IC50 value of 11.07 g.m/L. These findings suggest further examinations of this extract (e.g. assessment of off-target effects) are warranted.

Fractions 14 and 36K of Metabolite Extract Streptomyces hygroscopicus subsp. Hygroscopicus Have Antimalarial Activities Against Plasmodium berghei in vitro

“…Hygroscopicus strain i18 towards P. falciparum in vitro as measured using probit analysis was 11.07 μg/mL. 37 …”

“…Parasite density was calculated based on the number of infected red blood cells (IRBC) for an average of 5000 erythrocytes. The percentage of parasite density (%) was calculated by the formula below: 37 …”

“…While inhibition of parasite growth is expressed as a percentage, and calculated using the formula below. 37 …”

“…When the cells reached a high density about 80%, the cells could experiment by passing through their logarithmic growth phase. 37 …”

High Antiplasmodial Activity of Golden Gamat (S.hermanni) Extract Through In Vitro Study

“…Study groups G2 and G3, administration of chloroquine and S.hermanni extract will be separated into five dose groups: 100, 10, 1, 0.1, and 0.01 µg/ml [8]. Each dose will be repeated twice, resulting in 10 mediums with five different doses in each group.…”