In vitro testing of strawberry resistance to Verticillium dahliae and Phytophthora cactorum

Sowik, I.; Bielenin, A.; Michalczuk, Lech

doi:10.1016/s0304-4238(00)00195-3

Cited by 26 publications

(15 citation statements)

References 11 publications

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“…The fruit characteristics of the somaclone were not affected by selection but the plants were more vigorous than the plants of the original cultivar observed previously (Sowik et al 2001(Sowik et al , 2004a(Sowik et al ,b, 2007Sowik, Michalczuk 2002). In the present experiment we proved that high plant vigour was stable during 45 generations of in vitro shoot proliferations as well as during 4 generations of clonal propagation from runners.…”

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confidence: 75%

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Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones

Sowik¹,

Markiewicz²,

Michalczuk³

2015

Hort. Sci. (Prague)

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Sowik I., Markiewicz M., Michalczuk L. (2015): Stability of Verticillium dahliae resistance in tissue culturederived strawberry somaclones. Hort. Sci. (Prague), 42: 141-148.Somaclonal variation is a novel source of useful traits in strawberry breeding. In our previous work we selected K40 clone from in vitro cultures of strawberry cv. Elsanta, which was characterized by high resistance to Verticillium dahliae. The objective of this work was to evaluate the stability and transmission of this trait during in vitro shoot proliferation and traditional clonal propagation from runners. K40, Elsanta and Senga Sengana plants were propagated in vitro for 45 generations/passages or for 4 generation of clonal propagation from runners. The resistance/susceptibility of such derived plants to V. dahliae was tested in controlled conditions in a greenhouse. Irrespective of the method of propagation, the plants of K40 clone were much more resistant to V. dahliae than the plants of the original cultivar and the plants of 'Senga Sengana' , which is a cultivar considered as resistant to Verticillium wilt in field conditions. This proves that this trait elicited at somaclonal variant is stable and transferable both during in vitro micropropagation and clonal plant propagation from runners.

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confidence: 75%

“…A method for inducing and in vitro selecting somaclonal variants of strawberry with enhanced resistance to Verticillium dahliae has been developed (Sowik et al 2001). The clone K40, selected from tissue culture of strawberry cv.…”

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confidence: 99%

Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones

Sowik¹,

Markiewicz²,

Michalczuk³

2015

Hort. Sci. (Prague)

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“…Differences between in vitro and ex vitro conditions could account for the differences in response to C. acutatum. Such differences have been reported for strawberry in response to Phytophthora cactorum Sowik et al, 2001) and for apple in response to Erwinia amylovora (Visuer and Tapia y Figueroa, 1987), thus making it unacceptable to use an in vitro screening approach to evaluate that germplasm. Another explanation for the susceptibility of 'Delmarvel' in vitro could have been due to our using the highly virulent isolate Goff (Smith and Black, 1990;Denoyes and Baudry, 1995) compared with the C. acutatum used by Galletta et al (1995).…”

Section: Inoculation Evaluations For Strawberry Cultivarsmentioning

confidence: 95%

“…Taking the above into consideration, our results suggest that our in vitro assay with isolate Goff can be used to screen for anthracnose resistance in strawberry; thus, this same assay was used to screen strawberry regenerants from leaf explants of these same cultivars to this same pathogen. Sowik et al (2001) suggested that in vitro screening of strawberry could be useful for identifying somaclonal variants tolerant to infection with Verticillium dahliae, since they observed that the reaction of strawberry cultivars in vitro was similar to their sensitivity to Verticillium wilt in the field. However, this is in contrast to Gaggioli et al (1989) who were unsuccessful in developing an in vitro assay to screen for resistance to V. dahliae.…”

Section: Inoculation Evaluations For Strawberry Cultivarsmentioning

confidence: 99%

In vitro response of strawberry cultivars and regenerants to Colletotrichum acutatum

Hammerschlag¹,

Garcés²,

Koch-Dean³

et al. 2006

Plant Cell Tiss Organ Cult

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Diseases affecting strawberry (Fragaria Â ananassa Duch.) have been of major concern in recent years because of their widespread occurrence and potential for yield loss. Anthracnose, caused by the fungus Colletotrichum acutatum, is one of the most serious diseases of strawberry worldwide. Tissue-culture induced (somaclonal) variation provides one strategy for generating disease-resistant genotypes. As part of a program to generate strawberry germplasm resistant to anthracnose, an in vitro screening system was used to evaluate several commercial cultivars, Chandler, Delmarvel, Honeoye, Latestar, Pelican and Sweet Charlie propagated in vitro, and shoots regenerated from leaf explants of these cultivars for resistance to C. acutatum isolate Goff (highly virulent). Regenerants with increased levels of resistance were identified from all of the cultivars. The greatest increases in disease resistance were observed for regenerants from leaf explants of cultivars Pelican and Chandler that exhibited 17.5-and 6.2-fold increases in resistance, respectively. The highest levels of anthracnose resistance (2 to 6% leaf necrosis) were exhibited by regenerants from explants of cultivars Pelican and Sweet Charlie. These studies suggest that generating somaclonal variation may be a viable approach to obtaining strawberry plants with increased levels of anthracnose resistance.

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“…Results of inoculation performed on diploid Fragaria species showed no indication of any of these species being more resistant or susceptible than others to P. cactorum and no systematic differences resulting from geographic origin [9]. In the cultivated strawberry, F. x ananassa, variability of susceptibility of genotypes or cultivars was mainly reported in studies comparing different methods of inoculation, [10][11][12][13][14]. A single large study showed that resistance varied greatly between the 31 genotypes that were tested and offering high levels of resistance for further breeding programs [15].…”

Section: Introductionmentioning

confidence: 99%

Genetic variation of resistance of the cultivated strawberry to crown rot caused by Phytophthora cactorum

Schafleitner

Bonnet

Pedeprat

et al. 2013

Journal of Berry Research

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Evaluation of strawberry resistance to crown rot caused by Phytophthora cactorum is usually performed on a limited number of genotypes. The major objective of this study was to screen large genetic resources to identify potential parents that can be further used in breeding programs. Plants were inoculated by wounding the crown and placing a mycelium disk on the wound. Firstly, plug and cold stored plants were evaluated for their susceptibility to P. cactorum. Secondly, a total of 107 genotypes was evaluated using cold stored plants. Plug plants were very low affected by the wounding inoculation with P. cactorum whatever the genotype, whereas results obtained using cold stored plants consistently reflected the susceptibility of the genotype to crown rot. By using cold stored plants, we evaluated the susceptibility of 70 varieties and 37 advanced lines. Among the genotypes, we identified varieties such as Cirafine or Cireine with high level of resistance similar to the one of Senga Sengana. These data will be useful for choosing parents in breeding programs and for validation of markers linked to the resistance to P. cactorum.

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In vitro testing of strawberry resistance to Verticillium dahliae and Phytophthora cactorum

Cited by 26 publications

References 11 publications

Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones

Stability of Verticillium dahliae resistance in tissue culture-derived strawberry somaclones

In vitro response of strawberry cultivars and regenerants to Colletotrichum acutatum

Genetic variation of resistance of the cultivated strawberry to crown rot caused by Phytophthora cactorum

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