In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.)

Roy, Ashis Taru; Leggett, G. W.; Koutoulis, Anthony

doi:10.1007/s002990100364

Cited by 93 publications

(69 citation statements)

References 8 publications

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“…The production of pre-embryogenic callus from treated anthers took 5 months, almost twice the time required for untreated anthers. Similar impacts were detected by Roy et al (2001).…”

Section: Discussionsupporting

confidence: 85%

In vitro induction of tetraploids in Vitis × Muscadinia hybrids

Xie

Agüero

Wang

et al. 2015

Plant Cell Tiss Organ Cult

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A protocol for in vitro induction of tetraploids of the grape rootstock 101-14 Millardet et de Grasset (Vitis riparia 9 V. rupestris) (101-14 Mgt) (2n = 2x = 38) 9 Muscadinia rotundifolia cv. Trayshed (2n = 2x = 40) hybrids was established. Different explant materials were exposed to the antimitotic chemical agents colchicine and oryzalin, at three concentrations, for 24, 48 and 72 h. Treated in vitro shoot tips, anthers and pre-embryogenic cultures were regenerated, and their ploidy levels were confirmed by chromosome counts and flow cytometry. It is generally considered that anatomical characteristics of leaves, including leaf stomatal size and number of chloroplasts in guard cells, correlate with ploidy levels of grape plants. Chromosome counts and flow cytometry are labor intensive and difficult, while the number of chloroplasts in stomatal guard cells and stomatal size are easily measured and can be stable and reliable markers. Analysis of stomata revealed clear differences between regenerated diploid and tetraploid plants, and these measurements were used as a preliminary screen, followed by chromosome counts and flow cytometry for confirmation. This study presents the results obtained by various combinations of explants, antimitotic agents, exposure time, concentration and ploidy analysis to evaluate in vitro chromosome doubling. These results provide an easy and effective method for production of tetraploid, potentially fertile Vitis 9 Muscadinia hybrids for utilization in rootstock breeding programs.

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“…The production of pre-embryogenic callus from treated anthers took 5 months, almost twice the time required for untreated anthers. Similar impacts were detected by Roy et al (2001).…”

Section: Discussionsupporting

confidence: 85%

In vitro induction of tetraploids in Vitis × Muscadinia hybrids

Xie

Agüero

Wang

et al. 2015

Plant Cell Tiss Organ Cult

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“…Clones 93018, C19 and 18(9226) produced polyploid microshoots at the lowest (0.05-0.1%) colchicine concentrations, like in cocoyam (Tambong et al, 1998), mulberry (Chakraborti et al, 1998), lilac hybrids (Rose et al, 2000), and hop (Roy et al, 2001) polyploidisation experiments. However, for quince genotypes 43(9218), 9365, 47(9221) higher (0.6-0.9%) colchicine concentrations were most suitable.…”

Section: Polyploidisation Of Microshootsmentioning

confidence: 95%

In vitro induction of polyploidy in japanese quince (Chaenomeles japonica)

Stanys

Weckman

Stanienė

et al. 2006

Plant Cell Tiss Organ Cult

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In vitro conditions for Japanese quince polyploidisation were investigated. Microshoots and isolated cotyledons were treated with colchicine and oryzalin. Morphogenesis was more dependent on the concentration of colchicine or oryzalin than on the duration of exposure, genotype differences were observed. Low oryzalin concentrations had no impact on morphogenesis. Plants with changed chromosome numbers were obtained at 0.25-38 mM colchicine and 10-50 lM oryzalin concentrations. It was determined that stomata length is a suitable parameter for identifying putative Japanese quince tetraploids. Stomata of tetraploid shoots of the same clone were approximately 1/3 longer than in the diploids. It was shown that through polyploidisation gigas effect was not obtained in fruit size but tetraploids have reduced seed set and an increased proportion of fruit flesh.

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“…Anti-mitotic colchicine has proved to be an effective agent for inducing polyploidy, At present, polyploidy has been successfully induced in vivo or in vitro by colchicine in a series of crop species, including banana (Hamill et al 1992;Van Duren et al 1996), mulberry (Chakraborti et al 1998), corn (Barnabas et al 1999), ginger (Adaniya and Shira 2001), hops (Roy et al 2001), pear (Kadota and Niimi 2002), pomegranate (Shao et al 2003), Miscanthus sinensis (Petersen et al 2003), Bixa orellana (Carvalho et al 2005), Platanus acerifolia (Liu et al 2007),Vaccinium darrowii (Chavez and Lyrene 2009), Phlox subulata L. (Zhang et al 2008), and Helleborus (Dhooghe and Van Labeke 2007;Dhooghe et al 2009a, b). Among others, induction of polyploidy has been achieved in many Araceae members, such as Xanthosoma (Esnard et al 1993), Zantedeschia , Alocasia (Thao et al 2003), Spathiphyllum wallisii Regel (Eeckhaut et al 2004), Zamioculcas Zamiifolia (Vanzie-Canton and Leonhardt 2009).…”

Section: Introductionmentioning

confidence: 99%

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

et al. 2011

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Tetraploids plants of Anthurium andraeanum ''Arizona'' were successfully induced after treating diploid tissue masses with colchicine. Masses originating from diploid aerial roots were treated with colchicine at three different concentrations (i.e., 0.1, 0.2, 0.3%) for about 3, 5 and 7 h, and then were transferred into Murashige and Skoog medium containing 3 mg/l BAP ? 0.2 mg/l 2,4-D. After 60 days, the survival rate and numbers of regenerative shoots were scored. The high concentration and longer duration sharply reduced survival rate. In contrast, the regeneration of plantlets was not noticeably affected by colchicine. Tetraploid plants were obtained in all treatments, but the percentage of induced tetraploids ranged from 0.2 to 7.6%. The best induction was obtained with a 5-h, treatment with 0.3% colchicine. The stomatal size of tetraploid plants was larger than in diploid plants; however, the stomatal density was lower than in diploid plants. Tetraploid plants possessed stronger petioles, thicker and deeper green leaves, and thicker and longer lived spathes in comparison with diploid plants. Abnormal spathes, such as double spathes or those lacking pedicels, were observed in tetraploid plants. Tetraploid plantlets could be regenerated via aerial roots; this technique could be applied to tetraploid plant propagation.

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In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.)

Cited by 93 publications

References 8 publications

In vitro induction of tetraploids in Vitis × Muscadinia hybrids

In vitro induction of tetraploids in Vitis × Muscadinia hybrids

In vitro induction of polyploidy in japanese quince (Chaenomeles japonica)

Induction of Anthurium andraeanum “Arizona” tetraploid by colchicine in vitro

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