In Vitro Transformation by the Adenovirus-Sv40 Hybrid Viruses

Black, Paul H.; White, Beverly J.

doi:10.1084/jem.125.4.629

Cited by 32 publications

(8 citation statements)

References 31 publications

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“…12-transformed cells with the exception of one tumor with larger cells, vesicular nuclei, and prominent nucleoli more suggestive of SV40 tumor cells. Cytologic examination of these hybrid transformed cell lines in vitro revealed a morphologic picture which was similar to that of the tumors induced with these cell lines with the exception that tubule formation was not present in the in vitro preparations (1).…”

Section: Discussionmentioning

confidence: 82%

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In Vitro Transformation by the Adenovirus-Sv40 Hybrid Viruses

Igel

Black

1967

The Journal of Experimental Medicine

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Tumors induced with hamster kidney cells transformed by the adeno 2-, adeno 3-, adeno 7-, and adeno 12-SV40 hybrid viruses, and by adenovirus type 12, were examined histologically. The tumors induced with adeno 2-, adeno 3-, and adeno 7-SV40-transformed cells were similar to tumors induced with SV40-transformed hamster kidney cells but contained cells intermediate in morphology between SV40 and adenovirus tumor cells and occasionally contained nests of adenovirus-like cells. Cells transformed by the adeno 12-SV40 hybrid and by adenovirus type 12 gave rise to morphologically similar tumors. The results suggest that both viral genomes are operative in hybrid-transformed cells but that one genome is apparently responsible for the predominant morphology of the tumor. Evidence that the morphology of a single transformed target cell is determined by the transforming genome was discussed.

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Section: Discussionmentioning

confidence: 82%

“…2 +iT-, and Ad. 2++-transformed cells the SV40 genome predominates with respect to morphology and antigen content but prel~miuary evidence suggests that adeno antigens are present in these cells as well (1).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Transformation by the Adenovirus-Sv40 Hybrid Viruses

Igel

Black

1967

The Journal of Experimental Medicine

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“…Most of the Syrian hamster embryo cell lines transformed after exposure to chemical carcinogens [12,13], polyoma [14,15] and human adenoviruses [16,17] also remained in the diploid range during long-term cultivation but some of these cell lines shifted to heteroploidy. Syrian hamster embryo cells transformed with SV40 became heteroploid early in contrast with spontaneously transformed cells that remained pseudodiploid [18].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Chromosomes of Syrian Hamster Cells Transformed with Human Cytomegalovirus

Nachtigal¹,

Albrecht²,

Rapp³

1974

Intervirology

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A Syrian hamster embryo cell line derived after transformation by human cytomegalovirus (CMV) and 6 cell lines derived from 3 tumors and a metastasis induced by transplantation of the in vitro transformed cells into animals were analyzed using cytogenetic techniques. A spontaneously transformed hamster cell line was included in the study. The CMV-transformed cell lines grown in vitro kept diploid or near-diploid chromosome numbers and contained 2–20% cells with unstable structural chromosome aberrations. These were predominantly dicentrics and sometimes exhibited abnormal heterochromatic regions. In advanced passages one marker chromosome (mar 1) was found by Giemsa banding. The cell lines derived from tumors were near-diploid, mixoploid or heteroploid with chromosome numbers in the triploid-tetraploid range. They contained 24–40% cells with unstable structural chromosome aberrations including dicentrics, double-minutes and acentrics. A marker chromosome similar to mar 1 was found in 3 of these cell lines, and another marker was present in 4 cell lines. A reduction in the number of acrocentric (D) chromosomes was noticed in all the cell lines. Spontaneously transformed cells were hyperdiploid and contained acentrics and double-minutes but no dicentrics. These results suggest that CMV-transformed cells maintained an abnormal, but stable chromosome complement in vitro, but these became unstable when derived from tumors. Some marker chromosomes seemed stable.

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“…The adenovirus (Ad.) lines (Ad.HK and Ad.RE) were derived from primary weanling hamster kidney (Black and White, 1967) and primary rat embryo (Freeman et al, 1967a, b) monolayer cultures, respectively, transformed by Ad.12 and/or Ad.2. The hamster polyoma line (Py-T-54) and mouse polyoma line (Py-1923) were derived from tumors produced by inoculation of newborn hamsters or mice, respectively, with polyoma virus; these cell lines were obtained from Dr. K. Takemoto of the National Institutes of Health.…”

Section: Transformed Cell Linesmentioning

confidence: 99%

Induction experiments with adenovirus and polyoma virus transformed cell lines

Burns

Black

1969

Intl Journal of Cancer

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Techniques used successjully in inducing S V40-transjornied cells to produce ittfrctious SV40 were employed in an attempt to induce cells trarisjornied by other oncogenic D N A viruses, adenoviruses 2 and 12 arid polyomu virus. These techniques included cocultivation and fusion of transformed cells with indicator cells, and mitomycin C treatment of these heterokaryons and of transformed cells. I n addition, prior to induction experiments, some cell lines were passaged through animals or in the presence of BUdR in an effort to enhance their inducibility. AN attempts to induce these transformed cells were unsuccessful. It is conclurtd fiom this study that the polyomu and atlenovirus genomes, i f present in their entirety in cells transjormett with these viruses, arc not as reactivable as is the S V40 genome in S V40-traii,sj~~rnicrl cells.

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In Vitro Transformation by the Adenovirus-Sv40 Hybrid Viruses

Cited by 32 publications

References 31 publications

In Vitro Transformation by the Adenovirus-Sv40 Hybrid Viruses

In Vitro Transformation by the Adenovirus-Sv40 Hybrid Viruses

Analysis of Chromosomes of Syrian Hamster Cells Transformed with Human Cytomegalovirus

Induction experiments with adenovirus and polyoma virus transformed cell lines

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