In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)

Borghezan, Marcelo; Clauman, Alan David; Steinmacher, Douglas André; Guerra, Miguel Pedro; Orth, Afonso Inácio

doi:10.1590/s1984-70332011000400007

Cited by 35 publications

(16 citation statements)

References 21 publications

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“…Another research on Carica papaya L. "Washington" pollen which was freeze-dried and stored at −20˚C for 14 months showed that artificial pollination using stored pollen produced fruit set [21]. There are also similar studies on different species such as [22] and [23]. Although the importance of pollen storage about fertilization and germination abilities has been mentioned and the related studies were conducted, it seems that the researches about effects of stored pollens on fruit quality characters are not common or has not yet in-terested.…”

Section: Introductionmentioning

confidence: 99%

Effects of Stored Pollens from Wild <i>Actinidia eriantha</i> Vines on Some Fruit Quality Traits

Seyrek¹,

Luo²,

Zhong³

et al. 2017

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Our study was conducted to determine In vitro germination ability of pollens from 25 wild Actinidia eriantha genotypes after one year freeze storage, afterwards we examined fertilization ability of stored pollens of 8 genotypes selected according to In vitro test results from aforementioned 25 genotypes, and finally investigated effects of stored viable pollens from "MH67", "MH55", "MH48" and "MH45" genotypes on fruit quality characters of the female experimental plant "M3" as the main purpose. Non-pollinated "M3" kiwifruit plant was taken as control. We found that In vitro germination and fertilization ability of different stored pollen sources, and also fruit quality characters including dry matter, total sugar, titratable acid, vitamin C, total polyphenol, total flavonoid, chlorophyll ("a", "b", and total) and carotenoid contents except total soluble solid content were significantly different. MH67 and MH45 genotypes were evaluated as the most suitable pollenizers which can be recommended as new candidate cultivars because of long term storage ability and brought about desired fruit quality characters. They also will be investigated more at further breeding studies.

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Section: Introductionmentioning

confidence: 99%

Effects of Stored Pollens from Wild <i>Actinidia eriantha</i> Vines on Some Fruit Quality Traits

Seyrek¹,

Luo²,

Zhong³

et al. 2017

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“…The protracted storage time increases the portion of pollen grains able to germinate for nine wild rose species that showed viability higher or similar to that before storage at −25 °C for six months [ 12 ]. The kiwi pollen grains of the cultivar “Tomuri”, stored in the refrigerator or in the freezer, can be used for pollination in the same growth cycle [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars

Giovannini¹,

Macovei²,

Caser

et al. 2017

Plants

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In the cut flower market, traditional breeding is still the best way to achieve new rose cultivars. The geographical delocalization of cultivar constitution (generally made in Europe and North America) and plant cultivation (large areas in Africa and South America) represents a limit point for crossing and selection. Rose breeders often need to overcome geographical distances, resulting in asynchrony in flowering among crossing parents, by storing and sending pollen. Hence, a key aspect in breeding programs is linked to pollen availability and conservation, jointly with the identification of parameters related to pollen fertility. In this study we present the results of three different trials. In the first, pollen diameter and pollen viability were chosen as fertility predictors of 10 Rosa hybrida commercial cultivars. In the second trial, aliquots of dried pollen grains of six R. hybrida cultivar were stored under two different temperatures (freezer at T = −20 °C and deep freezer at T = −80 °C) and after a wide range of conservation period, their viability was measured. In the third trial, the effective fertilization capacity of frozen pollen of 19 pollen donor cultivars was evaluated during 2015 crossing breeding plan, performing 44 hybridizations and correlating the number of seeds and the ratio seeds/crossing, obtained by each cultivar, with in vitro pollen germination ability.

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“…In the absence of boron, pollen tubes show abnormalities like coiling and bursting. Boron impacts pollen germination and pollen tube growth in three ways: it promotes absorption of sugars; it increases oxygen uptake; and also it is involved in the synthesis of pectic material for the wall of the actively growing pollen tube [6,25]. The key factor in achieving successful germination and to manipulate pollen tube growth is the osmolarity of pollen germination solution [26].…”

Section: Pollen Viability Test After Storagementioning

confidence: 99%

Pollen Viability of Aeschynanthus tricolor Hook.

Gandadikusumah

Wawangningrum

Rahayu

2017

JTLS

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Aeschynanthus (Gesneriaceae) is climbing or trailing epiphytes from the tropical forests of South-East Asia. Several species are widely cultivated for their attractive flowers, which usually scarlet with long corolla tubes and probably bird pollinated.This study was carried out on Aeschynanthus tricolor Hook. to determine in vitro germinability of (1) pollen collected from flowers at anthesis, on the day before anthesis, or up to five days after anthesis and (2) pollen collected on the day of anthesis and then stored at -20 o C for 127 days. Germinability tests were carried out on pollen grains after stored for four hours in 10% sucrose solution. The highest in vitro germination percentage observed was pollen collected on the day of anthesis (96.3%) and the lowest was pollen collected on the fifth day after anthesis (5.6%). The germination percentage of pollen after stored at -20 o C was 98% at beginning and 22% after 127 days. From this research we can conclude that pollen of A. tricolor can be successfully collected and then stored for some time, will be practical benefit for plant breeding and conservation purposes.

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In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)

Cited by 35 publications

References 21 publications

Effects of Stored Pollens from Wild <i>Actinidia eriantha</i> Vines on Some Fruit Quality Traits

Effects of Stored Pollens from Wild <i>Actinidia eriantha</i> Vines on Some Fruit Quality Traits

Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars

Pollen Viability of Aeschynanthus tricolor Hook.

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In vitro viability and preservation of pollen grain of kiwi (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev)

Cited by 35 publications

References 21 publications

Effects of Stored Pollens from Wild &lt;i&gt;Actinidia eriantha&lt;/i&gt; Vines on Some Fruit Quality Traits

Effects of Stored Pollens from Wild &lt;i&gt;Actinidia eriantha&lt;/i&gt; Vines on Some Fruit Quality Traits

Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars

Pollen Viability of Aeschynanthus tricolor Hook.

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Product

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Effects of Stored Pollens from Wild <i>Actinidia eriantha</i> Vines on Some Fruit Quality Traits

Effects of Stored Pollens from Wild <i>Actinidia eriantha</i> Vines on Some Fruit Quality Traits