In vitro virulence determinants, comparative pathogenicity of <i>Diutina (Candida) mesorugosa</i> clinical isolates and literature review of the D. rugosa complex

Montoya, Alexandra M.; Luna-Rodríguez, Carolina E.; Gracia-Robles, Gabriela; Rojas, Olga C.; Treviño-Rangel, Rogelio de J.; González, Gloria M.

doi:10.1080/00275514.2019.1585161

Cited by 10 publications

(14 citation statements)

References 76 publications

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“…A high crude mortality rate (33-83%) is associated with candidemia caused by C. rugosa complex (Minces et al 2009). Moreover, a recent study identified some virulence features, including C. albicans -comparable biofilm formation, in C. mesorugosa (Montoya et al 2019). Put together, these call for more surveillance.…”

Section: Discussionmentioning

confidence: 99%

Candida (Diutina) mesorugosa in Non-Albicans Candida Species Clinical Isolates in South West Nigeria

Owoicho

Oguzie

Ogunbiyi

et al. 2020

Preprint

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Introduction The emerging role of non-albicans Candida species (NACS) as causal agents of mild to life-threatening mycoses is increasingly being appreciated. Some NACS are known for intrinsic resistance or reduced susceptibility to some antifungal drugs. To inform therapeutic options and management of candidiasis, we embarked on Candida species surveillance in Southwest Nigeria. Method We obtained retrospective yeast isolates from urogenital samples of patients in a tertiary hospital in south western Nigeria. Among 47 isolates identified phenotypically, we encountered a yeast which failed to produce pseudohyphae in human serum but was morphologically similar to Candida albicans. We characterized this yeast by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) region of the ribosomal DNA. Results A BLAST search and sequence homology identified the yeast as Candida (Diutina) mesorugosa, with a strong bootstrap. Conclusion Candida mesorugosa, a rarely isolated yeast from clinical samples worldwide, should be considered among potentially relevant NACS in Nigerian clinics. To the best our knowledge, this is the first report of Candida mesorugosa from a patient sample in Nigeria. This finding confirms the need for more specific methods, such as DNA sequencing, for extensive surveillance of NACS. Key words: Candida mesorugosa, Candida rugosa complex, Non-albicans Candida species surveillance, ribosomal DNA sequencing, NACS

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Section: Discussionmentioning

confidence: 99%

Candida (Diutina) mesorugosa in Non-Albicans Candida Species Clinical Isolates in South West Nigeria

Owoicho

Oguzie

Ogunbiyi

et al. 2020

Preprint

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“…Spores were separated completely from larger fragments of the hyphae by glass wool filtration, followed by two washing steps similar to the bacteria procedure and resuspended in 1 mL 0.9% NaCl solution. The number of conidia was determined by a hemocytometer and adjusted to 2 × 10 8 conidia•mL −1 [36].…”

Section: Injection Assaymentioning

confidence: 99%

“…An extensive literature review was conducted to condense putative candidates from our bacterial and fungal isolates, which were previously reported to have entomopathogenic potential. Four bacteria and two fungal species isolated from the BSF larval gut have already been described as putative insect pathogens in the literature: Microbacterium thalassium for Ostrinia nubilalis (Lepidoptera: Crambidae) [40], Alcaligenes faecalis, Klebsiella pneumonia, and Pseudomonas aeruginosa for Galleria mellonella (Lepidoptera: Pyralidae) [41][42][43], as well as Diutina rugosa for Anastrepha ludens (Diptera: Tephritidae) [36,44] and Fusarium solani for Dendroctonus frontalis (Coleoptera: Curculionidae) [45].…”

Section: Characterization Of Putative Entomopathogenic Candidates Fro...mentioning

confidence: 99%

“…Although there has not yet been a widespread outbreak in commercial breeding, the economic risk of diseases caused by entomopathogens has increased due to further upscaling of production [16,17,20,68,69]. For this reason, all bacterial and fungal isolates from the gut of PKM-fed larvae were examined for the mention of entomopathogenicity as part of an extensive literature search, whereby six putative candidates were identified [36,[40][41][42][43][44][45]. The intracoelomal injection of putative entomopathogenic bacteria and fungi demonstrated pronounced differences in the survival probability.…”

Section: Investigation Of Putative Entomopathogenic Isolatesmentioning

confidence: 99%

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Isolation of Bacterial and Fungal Microbiota Associated with Hermetia illucens Larvae Reveals Novel Insights into Entomopathogenicity

et al. 2022

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Larvae of the black soldier fly (BSF) Hermetia illucens are polyphagous feeders and show tremendous bioconversion capabilities of organic matter into high-quality insect biomass. However, the digestion of lignocellulose-rich palm oil side streams such as palm kernel meal (PKM) is a particular challenge, as these compounds are exceptionally stable and are mainly degraded by microbes. This study aimed to investigate the suitability of BSF larvae as bioconversion agents of PKM. Since the intestinal microbiota is considered to play a key role in dietary breakdown and in increasing digestibility, the bacterial and fungal communities of BSF larvae were characterized in a culture-dependent approach and screened for their putative entomopathogenicity. The lethality of six putative candidates was investigated using intracoelomal injection. In total, 93 isolates were obtained with a bacterial share of 74% that were assigned to the four phyla Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Members of the genera Klebsiella, Enterococcus, and Sphingobacterium are part of the core microbiome, as they were frequently described in the gut of Hermetia larvae regardless of diet, nutritional composition, or rearing conditions. With 75%, a majority of the fungal isolates belonged to the phylum Ascomycota. We identified several taxa already published to be able to degrade lignocelluloses, including Enterococcus, Cellulomonas, Pichia yeasts, or filamentous Fusarium species. The injection assays revealed pronounced differences in pathogenicity against the larvae. While Alcaligenes faecalis caused no, Diutina rugosa weak (23.3%), Microbacterium thalassium moderate (53.3%), and Pseudomonas aeruginosa and Klebsiella pneumoniae high (≥80%) lethality, Fusarium solani injection resulted in 100% lethality.

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“…However, in case of the disturbance of the host environment or immune dysfunction, the fungal burden will be higher than the commensal levels. Under such conditions, C. albicans can proliferate and invade deep sites and induce a high mortality rate [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

In vitro activities of Traganum nudatum and Mentha pulegium extracts combined with amphotericin B against Candida albicans in production of hydrolytic enzymes

Tefiani¹,

Seddiki²,

Mahdad³

2020

CMM

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Background and Purpose: Candida albicans is an important microorganism in the normal flora of a healthy subject; however, it has an expedient pathogenic character that induces hydrolytic virulence. Regarding this, the present study aimed to find an in vitro alternative that could reduce the virulence of this yeast. Materials and Methods: For the purpose of the study, the effect of amphotericin B (AmB) combined with the extract of Traganum nudatum (E1) or Mentha pulegium (E2) was evaluated against the hydrolytic activities of esterase, protease, and phospholipase. This effect was determined by calculating the minimum inhibitory concentration (MIC), used to adjust the extract/AmB mixtures in culture media. Results: The evaluated Pz values, which corresponded to the different enzymatic activities, showed a decrease in the hydrolytic activities of C. albicans strains after the addition of E1/AmB and E2/AmB combinations at descending concentrations (lower than the obtained MICs). Conclusion: Based on the findings, it would be possible to reduce the pathogenesis of this species without destabilizing the balance of the flora.

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In vitro virulence determinants, comparative pathogenicity of Diutina (Candida) mesorugosa clinical isolates and literature review of the D. rugosa complex

Cited by 10 publications

References 76 publications

Candida (Diutina) mesorugosa in Non-Albicans Candida Species Clinical Isolates in South West Nigeria

Candida (Diutina) mesorugosa in Non-Albicans Candida Species Clinical Isolates in South West Nigeria

Isolation of Bacterial and Fungal Microbiota Associated with Hermetia illucens Larvae Reveals Novel Insights into Entomopathogenicity

In vitro activities of Traganum nudatum and Mentha pulegium extracts combined with amphotericin B against Candida albicans in production of hydrolytic enzymes

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