In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer

Windus, Louisa; Kiss, Debra Lois; Glover, Tristan Thomas; Avery, Vicky M.

doi:10.1016/j.yexcr.2012.07.013

Cited by 29 publications

(34 citation statements)

References 39 publications

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“…In comparison with 3-D images of HeLa cells on the glass substrate, we confirmed that the cellular morphology in 3-D culture and on the glass substrate is substantially different, as also indicated in other reports. [36][37][38] Recently, it has been recognized that 3-D culture techniques enable us to mimic the in vivo environment and to preserve more closely important natural cellular functions and morphology. Therefore, volumetric observations of thick specimens in 3-D culture will become more important for biological studies in the future.…”

Section: Resultsmentioning

confidence: 99%

Saturated excitation microscopy for sub-diffraction-limited imaging of cell clusters

et al. 2013

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Saturated excitation (SAX) microscopy offers high-depth discrimination predominantly due to nonlinearity in the fluorescence response induced by the SAX. Calculation of the optical transfer functions and the edge responses for SAX microscopy revealed the contrast improvement of high-spatial frequency components in the sample structure and the effective reduction of background signals from the out-of-focus planes. Experimental observations of the edge response and x-z cross-sectional images of stained HeLa cells agreed well with theoretical investigations. We applied SAX microscopy to the imaging of three-dimensional cultured cell clusters and confirmed the resolution improvement at a depth of 40 μm. This study shows the potential of SAX microscopy for super-resolution imaging of deep parts of biological specimens.

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Section: Resultsmentioning

confidence: 99%

Saturated excitation microscopy for sub-diffraction-limited imaging of cell clusters

et al. 2013

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“…Spheroids were prepared from single-cell suspensions of prostate cancer cell lines, as previously described (17)(18)(19). In brief, cells at a density of 0.5×10 4 cells per ml in RPMI-1640 phenol red-free medium supplemented with 5% (v/v) charcoalstripped FBS were placed in the wells of a 96-well ultra-low attachment (ULA) plate (17)(18)(19)). The ULA plate was then centrifuged at 100 × g for 3 min at room temperature.…”

Section: Real-time Quantitative Polymerase Chain Reaction (Qpcr)mentioning

confidence: 99%

Galectin-3 Is Implicated in Tumor Progression and Resistance to Anti-androgen Drug Through Regulation of Androgen Receptor Signaling in Prostate Cancer

Dondoo

Fukumori

Daizumoto

et al. 2017

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“…As in the case of untransformed, normal cells, cancer cells also, when maintained in 3D culture, exhibit behavior and gene expression patterns closer to the in vivo conditions [77][78][79]. Already in the '70s, Bissel's group and others demonstrated how tissue architecture, 3D context, composition of ECM, correct cell polarization, and epithelial adhesion are fundamental for reproducing relevant in vitro models able to mirror, as much as possible, the physiological or pathological features of breast tissue [80,81]. They showed that the 3D culture better recapitulates different tumors' behavior than did 2D configurations.…”

Section: D Versus 3d Cancer Modelsmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer

Cited by 29 publications

References 39 publications

Saturated excitation microscopy for sub-diffraction-limited imaging of cell clusters

Saturated excitation microscopy for sub-diffraction-limited imaging of cell clusters

Galectin-3 Is Implicated in Tumor Progression and Resistance to Anti-androgen Drug Through Regulation of Androgen Receptor Signaling in Prostate Cancer

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

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