In vivo determination of muscle-derived stem cells in rat corpus cavernosum

Xu, Lucy; Xue, Boxin; Shan, Yuxi; Chen, D; Gao, Jerry; Yang, Dong-rong; Sun, Chuanyang; Cui, Yan

doi:10.4238/2015.august.21.1

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…On the other hand, LESWT could enhance stem cell proliferation, differentiation and paracrine 44454647. In addition, the types of stem cells detected around the cavernous vasculature, including perisinusoidal and subtunic penile progenitor cells48 and cavernous smooth muscle-derived stem cells,4950 could be induced to activate. Thus, LESWT induced angiogenesis and created a beneficial situation for the establishment of the stem cell niche.…”

Section: Discussionmentioning

confidence: 99%

Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats

Shan

Zhang

Chen³

et al. 2017

Asian J Androl

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Stem cell transplantation and low-energy shock-wave therapy (LESWT) have emerged as potential and effective treatment protocols for diabetic erectile dysfunction. During the tracking of transplanted stem cells in diabetic erectile dysfunction models, the number of visible stem cells was rather low and decreased quickly. LESWT could recruit endogenous stem cells to the cavernous body and improve the microenvironment in diabetic cavernous tissue. Thus, we deduced that LESWT might benefit transplanted stem cell survival and improve the effects of stem cell transplantation. In this research, 42 streptozotocin-induced diabetic rats were randomized into four groups: the diabetic group (n = 6), the LESWT group (n = 6), the bone marrow-derived mesenchymal stem cell (BMSC) transplantation group (n = 15), and the combination of LESWT and BMSC transplantation group (n = 15). One and three days after BMSC transplantation, three rats were randomly chosen to observe the survival numbers of BMSCs in the cavernous body. Four weeks after BMSC transplantation, the following parameters were assessed: the surviving number of transplanted BMSCs in the cavernous tissue, erectile function, real-time polymerase chain reaction, and penile immunohistochemical assessment. Our research found that LESWT favored the survival of transplanted BMSCs in the cavernous body, which might be related to increased stromal cell-derived factor-1 expression and the enhancement of angiogenesis in the diabetic cavernous tissue. The combination of LESWT and BMSC transplantation could improve the erectile function of diabetic erectile function rats more effectively than LESWT or BMSC transplantation performed alone.

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Section: Discussionmentioning

confidence: 99%

Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats

Shan

Zhang

Chen³

et al. 2017

Asian J Androl

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“…Yiou et al (2016) [44] studied the regenerative properties of MSCs and reported the ability of MSCs to repair multiple types of damage, especially those seen after RP. Also Xu et al (2015) [45] found that muscle-derived SCs in rat corpus cavernosum may have therapeutic potential in the treatment of organic ED. In a prevention study, Takayanagi et al (2015) [46] showed that i.v.…”

Section: Resultsmentioning

confidence: 99%

Innovative trends and perspectives for erectile dysfunction treatment: A systematic review

Ismail

El‐Sakka

2016

Arab Journal of Urology

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ObjectiveTo review contemporary knowledge concerning the innovative trends and perspectives in the treatment of erectile dysfunction (ED).MethodsMedline was reviewed for English-language journal articles between January 2000 and March 2016, using the terms ‘erectile dysfunction treatments’, ‘new trends’ and ‘perspectives’. In all, 114 original articles and 16 review articles were found to be relevant. Of the 76 cited papers that met the inclusion criteria, 51 papers had level of evidence of 1a–2b, whilst 25 had level of evidence of 3–4. Criteria included all pertinent review articles, randomised controlled trials with tight methodological design, cohort studies, and retrospective analyses. We also manually reviewed references from selected articles.ResultsSeveral interesting studies have addressed novel phosphodiesterase type 5 inhibitors (PDE5Is), orodispersible tablets, their recent chronic use, and combination with other agents. A few controlled studies have addressed herbal medicine as a sole or additional treatment for ED. Experimental studies and exciting review papers have addressed stem cells as novel players in the field of ED treatment. Other recent articles have revised the current status of low-intensity extracorporeal shockwave therapy in the field of ED. A few articles without long-term data have addressed new technologies that included: external penile support devices, penile vibrators, tissue engineering, nanotechnology, and endovascular tools for ED treatment.ConclusionsThe current treatment of ED is still far from ideal. We expect to see new drugs and technologies that may revolutionise ED treatment, especially in complex cases.

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“…Testosterone is an important inducer of stem cell differentiation [ 22 ], and endogenous muscle-derived stem cells have been detected in rat corpora cavernosa [ 23 ]. In vitro stem cell treatments can improve muscle content and decrease fibrosis in the corpora cavernosa [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of TNF-α on penile structure alteration in rats with hyperprolactinemia

Wang

Yang²,

Chen

et al. 2017

PLoS ONE

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This study aimed to investigate the effects of anti-tumor necrosis factor (TNF)-α antibody (Ab) on alteration of penile structure in the hyperprolactinemia (hyperPRL) rat model. HyperPRL was induced in 8-week-old male Sprague-Dawley rats by allografting anterior pituitary (AP) glands under the renal capsule (+AP rats). Rats implanted with cerebral cortex (CX) were used as sham control (+CX rats). At 6 weeks post implantation, rats received either a single intra-testicular dose of TNF-α Ab (12.5 μg/kg) or testosterone replacement (2 doses of testosterone enanthate [TE], 3 mg/kg), and they were sacrificed 1 week later. Blood and penile tissue was collected for analysis. Compared to +CX rats, the +AP group had lower serum testosterone concentration and neuronal nitric oxide synthase (nNOS) expression, but exhibited a higher ratio of collagen III/I in the corpus cavernosum. Smooth muscle content exhibited no significant changes. At 1 week post TNF-α Ab injection, the collagen III/I ratio in the +AP group was decreased, and the smooth muscle content and nNOS expression increased significantly. These findings were comparable to those observed in +AP rats receiving TE. Testicular TNF-α suppresses testosterone release, which in turn results in the erectile dysfunction (ED) seen in hyperPRL. Intra-testicular TNF-α Ab treatment is as effective as testosterone supplementation on penile structure normalization in the hyperPRL model.

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In vivo determination of muscle-derived stem cells in rat corpus cavernosum

Cited by 3 publications

References 24 publications

Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats

Combination of low-energy shock-wave therapy and bone marrow mesenchymal stem cell transplantation to improve the erectile function of diabetic rats

Innovative trends and perspectives for erectile dysfunction treatment: A systematic review

Effects of TNF-α on penile structure alteration in rats with hyperprolactinemia

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