In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

Yoshimura, Tetsuhiko; Yokoyama, Hidekatsu; Fujii, Satoshi; Takayama, Fusako; Oikawa, Kazuo; Kamada, Hitoshi

doi:10.1038/nbt0896-992

Cited by 231 publications

(140 citation statements)

References 22 publications

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“…The water-soluble iron-DTCS complex reacted with NO to give an [Fe II (DTCS) 2 (NO)] 2Ϫ (NO-iron-DTCS) complex with a three-line ESR signal (g ϭ 2.040; a N ϭ 1.27 mT) near 700 MHz and X-band frequencies at room temperature. Because the stable and water-soluble NO-iron-DTCS complex produces an intense ESR signal at room temperature, the iron-DTCS complex can be used as a spin-trapping reagent for in vivo NO assay (Yoshimura et al, 1996;Yasui et al, 2004). A three-line ESR signal (g ϭ 2.040; a N ϭ 1.27 mT) from mouse blood was observed 30 s after intravenous injection of PEG-poly SNO-BSA in mice (Fig.…”

Section: Resultsmentioning

confidence: 95%

Development of Polyethylene Glycol-Conjugated Poly-S-Nitrosated Serum Albumin, a NovelS-Nitrosothiol for Prolonged Delivery of Nitric Oxide in the Blood Circulation in Vivo

Katsumi¹,

Nishikawa²,

Yamashita³

et al. 2005

J Pharmacol Exp Ther

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S-Nitrosothiols are an interesting class of nitric oxide (NO) donors used for the treatment of circulation disorders. In this study, we developed a novel macromolecular NO donor in which 10 NO molecules were covalently bound to polyethylene glycol (PEG)-conjugated bovine serum albumin (BSA) through S-nitrosothiol linkages (PEG-poly SNO-BSA). Intermolecular disulfide linkages possibly formed during the introduction of thiol groups to BSA were prevented in PEG-poly SNO-BSA. Electron spin resonance study indicated that PEG-poly SNO-BSA does release the NO radical in the blood circulation in vivo. The area under the concentration-time curve of 111 In-PEG-poly N-succinimidyl S-acetylthioacetate (SATA)-BSA, the carrier part of PEG-poly SNO-BSA, was 1.7 times greater than that of 111 In-BSA after intravenous injection in mice. After intravenous injection in rats at an equivalent NO dose (3 mol of NO per kilogram), the duration of reduction in the blood pressure was 2.3 to 3.7 times longer in PEG-poly SNO-BSA than in classic S-nitrosothiols such as S-nitroso-N-acetyl penicillamine, S-nitrosoglutathione, and NO-BSA. The release half-life of NO from PEG-poly SNO-BSA was 11 to 108 times longer than those of the classic S-nitrosothiols examined, and this slow release rate of NO would explain the sustained reduction in the blood pressure after intravenous injection of PEG-poly SNO-BSA in rats. No cross-tolerance between PEG-poly SNO-BSA and nitroglycerin was also observed. These findings indicate that the novel S-nitrosothiol PEG-poly SNO-BSA is a promising compound that exhibits unique characteristics of sustained release of NO in the blood circulation in vivo, which would be beneficial for the treatment of circulation disorders.

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Section: Resultsmentioning

confidence: 95%

Development of Polyethylene Glycol-Conjugated Poly-S-Nitrosated Serum Albumin, a NovelS-Nitrosothiol for Prolonged Delivery of Nitric Oxide in the Blood Circulation in Vivo

Katsumi¹,

Nishikawa²,

Yamashita³

et al. 2005

J Pharmacol Exp Ther

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“…Yoshimura and coworkers were able to image NO in the abdominal section of a septic rat, although the large linewidth of the radical signal, ca. 3 Gauss, presents a limitation to the image resolution since the field gradients needed for such a study exceed those necessary for aminoxyl radicals [105]. Nonetheless, this is a unique demonstration of the power of EPR in 'directly' measuring a biological free radical in living animals.…”

Section: Nitric Oxide (No): a Naturally Occurring Free Radicalmentioning

confidence: 99%

The evolution of biomedical EPR (ESR)

Berliner

2017

BSI

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Abstract. Since the first EPR/ESR spectrum of a paramagnetic substance was published over 70 years ago, the technical improvements did not occur until after/during World War II with the advent of radar technology. The approaches to biomedical problems started somewhat later with the real burst of activity starting after the birth of the spin label technique about 50 years ago. The applications to proteins, then membranes and nucleic acids, and later applications to cells and eventually in-vivo on small animals and now humans has led EPR/ESR to finally being recognized as a uniquely powerful technique in the toolbox of techniques probing macromolecules and their interactions, free radical biology and its eventual value as a diagnostic technique. This article gives an overview of EPR/ESR studies of biomedically related systems, including proteins and enzymes. It presents a very personal historical perspective, briefly reviews the origins of the technique and reflects on possible future directions. As with NMR, advances in molecular biology and technology drastically changed the nature and focus of the technique, particularly the site directed spin labeling method that has been invaluable in determining protein and macromolecular structure by both EPR and NMR.

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“…An iron-dithiocarbamate-Fe[II] complex is typically used as the NO 'spin trap'. For example, diethyldithiocarbamate (DETC), 25 N-methyl-D-glucamine dithiocarbamate (MGD) 26 and N-dithiocarboxy sarcosine (DTCS) 27 are common trapping agents.…”

Section: Practical Application Of In Vivo Esr Methodology: Detection mentioning

confidence: 99%

Detection of bioradicals by in vivo L‐band electron spin resonance spectrometry

Fujii

Berliner

2004

NMR in Biomedicine

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The applications of in vivo electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy have been impressive over a relatively short period despite the many obstacles which had to be overcome, such as dielectric absorption and biodestruction. The loop-gap resonators and modified loop coil systems have emerged as the most suitable resonators for in vivo EPR experiments. This paper briefly discusses instrumental aspects as a prelude to several examples related to the in vivo monitoring and detection of bioradicals. Recent progress in detection of bioradicals induced by drugs or chemicals is discussed with regard to nitrosocompounds, nitric oxide and metals in vivo. A clinical EPR application is also discussed.

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In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice

Cited by 231 publications

References 22 publications

Development of Polyethylene Glycol-Conjugated Poly-S-Nitrosated Serum Albumin, a NovelS-Nitrosothiol for Prolonged Delivery of Nitric Oxide in the Blood Circulation in Vivo

Development of Polyethylene Glycol-Conjugated Poly-S-Nitrosated Serum Albumin, a NovelS-Nitrosothiol for Prolonged Delivery of Nitric Oxide in the Blood Circulation in Vivo

The evolution of biomedical EPR (ESR)

Detection of bioradicals by in vivo L‐band electron spin resonance spectrometry

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