In vivo genotoxicity evaluation of atrazine and atrazine–based herbicide on fish Carassius auratus using the micronucleus test and the comet assay

Çavaş, Tolga

doi:10.1016/j.fct.2011.03.038

Cited by 158 publications

(86 citation statements)

References 31 publications

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“…Srivastava and Mishra [102] observed results that are in agreement with the findings of Zeljezic et al [101] and Çavas [84], in which the exposure to different concentrations of Gesaprim inhibited the mitotic index and increased the frequencies of micronuclei and chromosome aberrations in somatic cells of Allium cepa and Vicia faba. [20].…”

Section: Atrazinesupporting

confidence: 84%

“…A study carried out by Çavas [84] compared the genotoxic effects of the active ingredient atrazine and its commercial formulation Gesaprim, in the concentrations of 5, 10 and 15 µg/L, by the comet assay and micronucleus test, in erythrocytes of the fish gibel carp (Carassius auratus). The results showed that there was a significant increase in the frequencies of the micronuclei and DNA strand breaks in the erythrocytes treated with all the concentrations of the commercial formulation of atrazine, showing the genotoxic and mutagenic potential of Gesaprim for this species of fish.…”

Section: Atrazinementioning

confidence: 99%

See 1 more Smart Citation

Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health

Marin-Morales¹,

Camargo²,

Hoshina³

2013

Herbicides - Current Research and Case Studies in Use

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Section: Atrazinesupporting

confidence: 84%

Section: Atrazinementioning

confidence: 99%

Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health

Marin-Morales¹,

Camargo²,

Hoshina³

2013

Herbicides - Current Research and Case Studies in Use

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“…For instance, in an experiment undertaken with two species of cichlids (Palhares and Grisolia 2002), it was verified that fish injected intra-abdominally with either cyclophosphamide or mitomycin C significantly raised the MN rates in erythrocytes within 1 day. Studies with common water pollutants have corroborated those with reference genotoxicants, as in the case of a waterborne assay with an atrazine-based herbicide, which evoked MN in erythrocytes on the goldfish (Carassius auratus) in just 2 days (Cavas 2011). On the other hand, withdrawal of genotoxicant exposure may also result on the decrease of ENMAs, as shown for example in an experiment with the rainbow trout (Oncorhynchus mykiss), in which after just a 96-h recovery in clean water the rate of MN decreased (Bagdonas and Vosylienė 2006).…”

Section: Discussionmentioning

confidence: 79%

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Carrola

Santos

Rocha

et al. 2014

Environ Sci Pollut Res

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Fish are bioindicators of water pollution, and an increased rate of their erythrocyte nuclear morphological abnormalities (ENMAs)-and particularly of erythrocyte micronuclei (EMN)-is used as a genotoxicity biomarker. Despite the potential value of ENMAs and MN, there is scarce information about fish captured in Iberian estuaries. This is the case of the Portuguese estuaries of the Mondego, Douro and Ave, suffering from different levels of environmental stress and where chemical surveys have been disclosing significant amounts of certain pollutants. So, the aim of this study was to evaluate genotoxicants impacts and infer about the exposure at those ecosystems, using the grey mullet (Mugil cephalus) as bioindicator and considering the type and frequency of nuclear abnormalities of erythrocytes as proxies of genotoxicity. Sampling of mullets was done throughout the year in the important Mondego, Douro and Ave River estuaries (centre and north-western Portugal). The fish (total n=242) were caught in campaigns made in spring-summer and autumnwinter, using nets or fishing rods. The sampled mullets were comparable between locations in terms of the basic biometric parameters. Blood smears were stained with Diff-Quik to assess the frequencies of six types of ENMAs and MN (given per 1,000 erythrocytes). Some basic water physicochemical parameters were recorded to search for fluctuations matching the ENMAs. Overall, the most frequent nucleus abnormality was the polymorphic type, sequentially followed by the blebbed/ lobed/notched, segmented, kidney shaped, vacuolated, MN and binucleated. The total average frequency of the ENMAs ranged from 73‰ in the Mondego to 108‰ in the Ave. The polymorphic type was typically ≥50 % of the total ENMAs, averaging about 51‰, when considering all three estuaries. The most serious lesion-the MN-in fish from Mondego and Douro had a similar frequency (≈0.38‰), which was significantly lower than that in the Ave (0.75‰). No significant seasonal differences existed as to the MN rates and seasonal differences existed almost only in the Douro, with the higher values in AW. In general, the pattern of ENMAs frequencies was unrelated with the water physicochemical parameters. Considering the data for both the total ENMAs and for each specific abnormality, and bearing in mind that values of MN in fish erythrocytes >0.3‰ usually reflect pollution by genotoxicants, it is suggested that mullets were likely being chronically exposed to such compounds, even in the allegedly less polluted ecosystem (Mondego). Moreover, data supported the following pollution

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“…Different species of fish are used for evaluating pesticides and the choice of blood (erythrocyte) is made primarily because these cells are easily collected without sacrificing the animal and do not require additional separation methods [24] [25]. The use of different IMI concentrations caused a statistically significant increase (p < 0.05) in damage to the DNA molecule.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic Potential of the Insecticide Imidacloprid in a Non-Target Organism (<i>Oreochromis niloticus</i>-Pisces)

Ansoar-Rodríguez¹,

Christofoletti²,

Marcato³

et al. 2015

JEP

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The indiscriminate use of pesticides has become a serious environmental concern. Insecticide imidacloprid (IMI) is the second most widely used pesticides worldwide. In 2010, 1.934 tons of IMI were sold in Brazil, mostly to be used in sugarcane crops. Several studies have detected its presence in the aquatic environment constituting a risk to non-target organisms. The aquatic animals are organisms used for environmental biomonitoring worldwide. They are considered excellent genetic models to detect environmental mutagens. Among animal species, the fish Oreochromis niloticus, commonly known as Nile tilapia, has been used to evaluate DNA damage. The present study therefore evaluated the effect of IMI on the genetic material of Oreochromis niloticus (Pisces) erythrocytes exposed to different concentration (250; 125 and 62.5 µg/L) of IMI used in growing sugarcane. The effect of the IMI was measured using the comet assay and the micronucleus (MN) test, assays that detected genotoxic damage. The results in the comet assay demonstrated that the concentrations tested induced primary damage to DNA. They also proved the occurrence of MN and nuclear abnormalities at the higher concentration used in the micronuclei and other nuclear abnormalities test. The insecticide IMI induced primary DNA damage at all concentrations and

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In vivo genotoxicity evaluation of atrazine and atrazine–based herbicide on fish Carassius auratus using the micronucleus test and the comet assay

Cited by 158 publications

References 31 publications

Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health

Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Genotoxic Potential of the Insecticide Imidacloprid in a Non-Target Organism (<i>Oreochromis niloticus</i>-Pisces)

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In vivo genotoxicity evaluation of atrazine and atrazine–based herbicide on fish Carassius auratus using the micronucleus test and the comet assay

Cited by 158 publications

References 31 publications

Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health

Toxicity of Herbicides: Impact on Aquatic and Soil Biota and Human Health

Frequency of micronuclei and of other nuclear abnormalities in erythrocytes of the grey mullet from the Mondego, Douro and Ave estuaries—Portugal

Genotoxic Potential of the Insecticide Imidacloprid in a Non-Target Organism (&lt;i&gt;Oreochromis niloticus&lt;/i&gt;-Pisces)

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Genotoxic Potential of the Insecticide Imidacloprid in a Non-Target Organism (<i>Oreochromis niloticus</i>-Pisces)