In Vivo Glucose Measurement by Surface-Enhanced Raman Spectroscopy

Stuart, Douglas A.; Yuen, Jonathan M.; Shah, Nilam C.; Lyandres, Olga; Yonzon, Chanda Ranjit; Glucksberg, Matthew R.; Walsh, Joseph T.; Duyne, Richard P. Van

doi:10.1021/ac061238u

Cited by 326 publications

(266 citation statements)

References 32 publications

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“…Conjugation of monoclonal antibodies with SERS nanoparticles for targeting and imaging of specific cancer markers in live cultured cells was also recently investigated (5). Another study presents the first in vivo application of SERS for glucose measurements in a rat (22) by s.c. implantation of functionalized SERS nanospheres.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Keren

Zavaleta

Cheng

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

563

475

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Molecular imaging of living subjects continues to rapidly evolve with bioluminescence and fluorescence strategies, in particular being frequently used for small-animal models. This article presents noninvasive deep-tissue molecular images in a living subject with the use of Raman spectroscopy. We describe a strategy for small-animal optical imaging based on Raman spectroscopy and Raman nanoparticles. Surface-enhanced Raman scattering nanoparticles and single-wall carbon nanotubes were used to demonstrate whole-body Raman imaging, nanoparticle pharmacokinetics, multiplexing, and in vivo tumor targeting, using an imaging system adapted for small-animal Raman imaging. The imaging modality reported here holds significant potential as a strategy for biomedical imaging of living subjects.nanotubes ͉ SERS nanoparticles M olecular imaging of living subjects provides the ability to study cellular and molecular processes that have the potential to impact many facets of biomedical research and clinical patient management (1-4). Imaging of small-animal models is currently possible by using positron emission tomography (PET), single photon emission computed tomography, magnetic resonance imaging, computed tomography, optical bioluminescence and fluorescence, high frequency ultrasound, and several other emerging modalities. However, no single modality currently meets the needs of high sensitivity, high spatial and temporal resolution, high multiplexing capacity, low cost, and high-throughput.Fluorescence imaging, in particular, has significant potential for in vivo studies but is limited by several factors (5, 6), including a limited number of fluorescent molecular imaging agents available in the near infra-red (NIR) window with large spectral overlap between them, which restricts the ability to interrogate multiple targets simultaneously (multiplexing). In addition, background autofluorescence emanating from superficial tissue layers restricts the sensitivity and the depth to which fluorescence imaging can be used. Moreover, rapid photobleaching of fluorescent molecules limits their useful lifetime and prevents studies of prolonged duration. Therefore, we have attempted to develop new strategies that may solve some of the limitations of fluorescence imaging in living subjects.Raman spectroscopy can differentiate the spectral fingerprint of many molecules, resulting in very high multiplexing capabilities. Narrow spectral features are easily separated from the broadband autofluorescence, because Raman is a scattering phenomenon as opposed to absorption/emission in fluorescence, and Raman active molecules are more photostable compared with fluorophores, which are rapidly photobleached. Unfortunately, the precise mechanism for photobleaching is not well understood. However, it has been linked to a transition from the excited singlet state to the excited triplet state. Photobleaching is significantly reduced for single molecules adsorbed onto metal particles because of the rapid quenching of excited electrons by the metal surface...

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Section: Discussionmentioning

confidence: 99%

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Keren

Zavaleta

Cheng

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

563

475

View full text Add to dashboard Cite

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“…Noble metal nanoparticles provide remarkable opportunities in these applications due to their inherently low toxicity [31][32][33] and strongly enhanced optical properties associated with localized surface plasmon resonance [34][35][36]. The enhanced electromagnetic field surrounding such particles gives rise to large absorption, Rayleigh (Mie) scattering, raman scattering, and twophoton luminescence cross-sections, properties which have been utilized in photothermal cancer therapy [24][25][26][27][28][29][30] (PTT), surface enhanced Raman detection [37][38][39] (SERS), and diagnostic imaging [17][18][19][20] applications.…”

Section: Introductionmentioning

confidence: 99%

Gold nanorod assisted near-infrared plasmonic photothermal therapy (PPTT) of squamous cell carcinoma in mice

et al. 2008

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Plasmonic photothermal therapy (PPTT) is a minimally-invasive oncological treatment strategy in which photon energy is selectively administered and converted into heat sufficient to induce cellular hyperthermia. The present work demonstrates the feasibility of in vivo PPTT treatment of deep-tissue malignancies using easily-prepared plasmonic gold nanorods and a small, portable, inexpensive near-infrared (NIR) laser. Dramatic size decreases in squamous cell carcinoma xenografts were observed for direct (P<0.0001) and intravenous (P<0.0008) administration of pegylated gold nanorods in nu/nu mice. Inhibition of average tumor growth for both delivery methods was observed over a 13-day period, with resorption of >57% of the directly-injected tumors and 25% of the intravenously-treated tumors.

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“…FON substrates have broader LSPR resonances compared with NSL nanoprism arrays; however, they provide a large surface area available for binding and detection. 16 Functionalization of FON substrates is a crucial feature of sensing experiments and these modified surfaces have been used for in vitro [17][18][19] and in vivo 20 glucose sensing and anthrax biomarker detection. 21,22 SERS activity is affected by the oxidation of the metal, the lack of stability of the nanostructures when exposed to buffer solutions, and annealing at high temperatures.…”

mentioning

confidence: 99%

Controlled Plasmonic Nanostructures for Surface-Enhanced Spectroscopy and Sensing

et al. 2008

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After its discovery more than 30 years ago, surface-enhanced Raman spectroscopy (SERS) was expected to have major impact as a sensitive analytical technique and tool for fundamental studies of surface species. Unfortunately, the lack of reliable and reproducible fabrication methods limited its applicability. In recent years, SERS has enjoyed a renaissance, and there is renewed interest in both the fundamentals and applications of SERS. New techniques for nanofabrication, the design of substrates that maximize the electromagnetic enhancement, and the discovery of single-molecule SERS are driving the resurgence of this field.This Account highlights our group's recent work on SERS. Initially, we discuss SERS substrates that have shown proven reproducibility, stability, and large field enhancement. These substrates enable many analytical applications, such as anthrax detection, chemical warfare agent stimulant detection, and in vitro and in vivo glucose sensing. We then turn to a detailed study of the wavelength and distance dependence of SERS, which further illustrate predictions obtained from the electromagnetic enhancement mechanism. Last, an isotopic labeling technique applied to the rhodamine 6G (R6G)/silver system serves as an additional proof of the existence of singlemolecule SERS and explores the dynamical features of this process. This work, in conjunction with theoretical calculations, allows us to comment on the possible role of charge transfer in the R6G/silver system.

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In Vivo Glucose Measurement by Surface-Enhanced Raman Spectroscopy

Cited by 326 publications

References 32 publications

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Noninvasive molecular imaging of small living subjects using Raman spectroscopy

Gold nanorod assisted near-infrared plasmonic photothermal therapy (PPTT) of squamous cell carcinoma in mice

Controlled Plasmonic Nanostructures for Surface-Enhanced Spectroscopy and Sensing

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