In vivo NIR imaging with CdTe/CdSe quantum dots entrapped in PLGA nanospheres

Kim, Jin-Soo; Cho, Kwang Jae; Trần, Thanh Huyền; Nurunnabi, Md; Moon, Taeho; Hong, Suk Min; Lee, Yong-Kyu

doi:10.1016/j.jcis.2010.08.053

Cited by 61 publications

(43 citation statements)

References 38 publications

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“…3,35 In brief, to obtain the CdTe/CdSe QDs, a mixture of CdO (0.10 mmol), ODPA (0.205 mmol), and TOA (10 mL) was heated to 300 o C to obtain a clear solution. Tellurium solution (0.2 mmol, dissolved in 0.475 g of TOP) was injected quickly into this hot solution, and the reaction mixture was allowed to cool down to 250 o C for growing of CdTe nanocrystals.…”

Section: Synthesis and Characterization Of Qds Water Soluble Qds (W-mentioning

confidence: 99%

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Optical imaging, biodistribution and toxicity of orally administered quantum dots loaded heparin-deoxycholic acid

et al. 2015

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Quantum dots (QDs) are considered to be one of the most promising optical imaging probes for biological and biomedical applications. However toxicology is the major concern that limits biological application. Though couple of studies have reported focusing on intravenous administration but, in this study, we have observed toxicity of orally administered QDs for the very first time. QDs were loaded into heparin-conjugated deoxycholic acid (Q-LHD) conjugates. The orally administered Q-LHD nanoparticles were absorbed through bile acid transporter of small intestine. In vitro genotoxicity was observed by chromosomal aberration and comet assay to learn effect of quantum dot in cell mutation. The amount of cadmium content in different organs was also measured by inductive coupled plasma mass spectroscopy. To observe physiological changes, complete blood count, serum biochemistry and organ histology of Q-LHD treated rats were performed accordingly. The in vivo biodistribution results confirm any acute toxicity or significant variations were noted in the rats. Furthermore, oral administration of the Q-LHD nanoparticles does not cause appreciable toxicity, showing no mentionable histological changes of the organs at 45 days after single dose. Though this current study reveals, potential toxicity of Q-LHD nanoparticles is no longer a limiting factor for extending application of QDs in biomedical imaging but long term in vivo genotoxicity studies further required.

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Section: Synthesis and Characterization Of Qds Water Soluble Qds (W-mentioning

confidence: 99%

“…[1][2][3][4][5][6][7] These properties, especially their excellent photo-stability and size-tunable fluorescence wavelength, make them suitable for in vivo biomedical and in vitro cells imaging. [8][9][10][11][12][13] There are a significant number of studies conducted among researchers that lead the recent developments in QDs technology.…”

Section: Introductionmentioning

confidence: 99%

Optical imaging, biodistribution and toxicity of orally administered quantum dots loaded heparin-deoxycholic acid

et al. 2015

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“…The QDs capped with carboxyl ligands often produce intermediate agglomeration due to instability in neutral/acidic buffers. 32,33 It is proposed that QDs are released from liposomes after fusion with cell membranes. The intracellular pH of rat brain endothelial cells is reported to be 7.0, 34 which is lower than the physiological environment.…”

Section: 15mentioning

confidence: 99%

Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging

Wen¹,

Zhang²,

Al‐Suwayeh³

et al. 2012

IJN

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Quantum dots (QDs) and apomorphine were incorporated into liposomes to eliminate uptake by the liver and enhance brain targeting. We describe the preparation, physicochemical characterization, in vivo bioimaging, and brain endothelial cell uptake of the theranostic liposomes. QDs and the drug were mainly located in the bilayer membrane and inner core of the liposomes, respectively. Spherical vesicles with a mean diameter of ∼140 nm were formed. QDs were completely encapsulated by the vesicles. Nearly 80% encapsulation percentage was achieved for apomorphine. A greater fluorescence intensity was observed in mouse brains treated with liposomes compared to free QDs. This result was further confirmed by ex vivo imaging of the organs. QD uptake by the heart and liver was reduced by liposomal incorporation. Apomorphine accumulation in the brain increased by 2.4-fold after this incorporation. According to a hyperspectral imaging analysis, multifunctional liposomes but not the aqueous solution carried QDs into the brain. Liposomes were observed to have been efficiently endocytosed into bEND3 cells. The mechanisms involved in the cellular uptake were clathrin-and caveolamediated endocytosis, which were energy-dependent. To the best of our knowledge, our group is the first to develop liposomes with a QD-drug hybrid for the aim of imaging and treating brain disorders.

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“…The most frequent use of biosensors so far has been in blood glucose monitoring [5, 6]. Enzymes, antibodies, and receptors have been widely used in biosensors as biological sensing elements [7]. Biosensors have also shown potential for in vivo sensing of disease-specific biomarkers [8].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Application of Biosensors in Tissue Engineering

Hasan

Nurunnabi

Morshed

et al. 2014

BioMed Research International

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153

122

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Biosensors research is a fast growing field in which tens of thousands of papers have been published over the years, and the industry is now worth billions of dollars. The biosensor products have found their applications in numerous industries including food and beverages, agricultural, environmental, medical diagnostics, and pharmaceutical industries and many more. Even though numerous biosensors have been developed for detection of proteins, peptides, enzymes, and numerous other biomolecules for diverse applications, their applications in tissue engineering have remained limited. In recent years, there has been a growing interest in application of novel biosensors in cell culture and tissue engineering, for example, real-time detection of small molecules such as glucose, lactose, and H2O2 as well as serum proteins of large molecular size, such as albumin and alpha-fetoprotein, and inflammatory cytokines, such as IFN-g and TNF-α. In this review, we provide an overview of the recent advancements in biosensors for tissue engineering applications.

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In vivo NIR imaging with CdTe/CdSe quantum dots entrapped in PLGA nanospheres

Cited by 61 publications

References 38 publications

Optical imaging, biodistribution and toxicity of orally administered quantum dots loaded heparin-deoxycholic acid

Optical imaging, biodistribution and toxicity of orally administered quantum dots loaded heparin-deoxycholic acid

Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging

Recent Advances in Application of Biosensors in Tissue Engineering

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