2019
DOI: 10.1111/resp.13507
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In vivo probe‐based confocal laser endomicroscopy in chronic interstitial lung diseases: Specific descriptors and correlation with chest CT

Abstract: Background and objective: Probe-based confocal laser endomicroscopy (pCLE) enables in vivo microimaging of the distal lung, during bronchoscopy. This study aims at identifying pCLE descriptors of chronic interstitial lung diseases (ILD), their correlations with chest HRCT and assessing inter-observer agreement. Methods: pCLE was performed in 21 healthy volunteers (HV) and 59 non-smoking ILD patients, including 19 patients with idiopathic pulmonary fibrosis (IPF) or asbestosis, 15 with connective tissue disease… Show more

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Cited by 23 publications
(32 citation statements)
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“…Of note, while traditionally relatively high in adult human lungs, this spontaneous autofluorescence is low to almost undetectable in young rat lung tissues depending on the setting and type of CLE device in use (see below). Additional contrast and information can be gained by the topical or systemic administration of optical fluorescent probes (a few are FDA-approved for human use: e.g., fluorescein, indocyanin green (ICG), methylene blue (MB), cresyl violet) [51,52] or fluorophore-bound macromolecules (e.g., albumin or moderate-to-high molecular weight polysaccharides such as dextran) to visualize and monitor microcirculation and possible real-time extravascular fluid leakage [19,52,53] (Figure 2) which is an essential component of ARDS. Additionally, agglutinins or specific antibodies fragments can specifically enhance the information provided by endomicroscopy imaging [52,54].…”
Section: Advantages Limitations and Future Directions For In Vivmentioning
confidence: 99%
“…Of note, while traditionally relatively high in adult human lungs, this spontaneous autofluorescence is low to almost undetectable in young rat lung tissues depending on the setting and type of CLE device in use (see below). Additional contrast and information can be gained by the topical or systemic administration of optical fluorescent probes (a few are FDA-approved for human use: e.g., fluorescein, indocyanin green (ICG), methylene blue (MB), cresyl violet) [51,52] or fluorophore-bound macromolecules (e.g., albumin or moderate-to-high molecular weight polysaccharides such as dextran) to visualize and monitor microcirculation and possible real-time extravascular fluid leakage [19,52,53] (Figure 2) which is an essential component of ARDS. Additionally, agglutinins or specific antibodies fragments can specifically enhance the information provided by endomicroscopy imaging [52,54].…”
Section: Advantages Limitations and Future Directions For In Vivmentioning
confidence: 99%
“…report on the use of probe‐based confocal laser endomicroscopy (pCLE) to identify morphological abnormalities apparent on direct visualization of alveoli and terminal bronchioles in patients with ILD (e.g. presence of fluorescent cells in bronchiolar areas and increased density of alveolar elastic fibres) . Fourteen variables, selected based on preliminary observations in ILD patients and healthy volunteers, were detectable with good to excellent inter‐observer agreement (with kappa values ranging from 0.66 to 0.97).…”
mentioning
confidence: 99%
“…To date, we know of no other quantitative analyses for the intricacies of a structure such as the presented structural connectivity value. Other observed structural changes [30] such as alveolar mouth size and increase of fluorescent were not taken into consideration in the presented methods.…”
Section: Plos Onementioning
confidence: 99%
“…Previous studies have focused on expert observer evaluation [14] or manual post processing methods [30,31] when comparing different elastin of the alveoli. The presented method instead offers quantitative values where the whole structure inside the FOV can be evaluated.…”
Section: Plos Onementioning
confidence: 99%
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