In Vivo Solution-State NMR-Based Environmental Metabolomics

Majumdar, Rudraksha Dutta; Akhter, Mohammad; Fortier-McGill, Blythe; Soong, Ronald; Liaghati-Mobarhan, Yalda; Simpson, André J.; Spraul, Manfred; Schmidt, Sebastian; Heumann, Hermann

doi:10.1002/9780470034590.emrstm1533

Cited by 40 publications

(206 citation statements)

References 70 publications

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“…magna and H . azteca are provided elsewhere . Specifically for NMR studies, the organisms can be enriched by replacing their normal food source (algae) withC‐enriched algae.…”

Section: Sample Preperation Handling and Spinningmentioning

confidence: 99%

“…This is performed by passing the organisms through a mesh and acid/base washing the empty containers and rinsing thoroughly. Isotopic enrichment is recommended from birth for at least 3–4 weeks prior to the studies . It is important to remember that if the organisms have been recently fed, their 13 C‐enriched stomach contents will show up in the 1 H‐ 13 C NMR spectra.…”

Section: Sample Preperation Handling and Spinningmentioning

confidence: 99%

“…It is important to remember that if the organisms have been recently fed, their 13 C‐enriched stomach contents will show up in the 1 H‐ 13 C NMR spectra. If this is undesirable, then the guts can be purged using 12 C algae as described by Dutta Majumdar et al…”

Section: Sample Preperation Handling and Spinningmentioning

confidence: 99%

“…In high‐field NMR systems, there are two main approaches: (a) flow‐based NMR using solution state NMR probes and (b) magic angle spinning (MAS) based approaches. Flow‐based approaches provide a low stress environment, in which the organisms can be maintained indefinitely and is thus ideal for looking at metabolic responses to external stimuli. A separate tutorial in this special issue covers applications of flow‐based NMR, and therefore, it will not be discussed further here.…”

Section: Introductionmentioning

confidence: 99%

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In vivo comprehensive multiphase NMR

Mobarhan

Soong

Lane

et al. 2019

Magnetic Reson in Chemistry

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Traditionally, due to different hardware requirements, nuclear magnetic resonance (NMR) has developed as two separate fields: one dealing with solids, and one with solutions. Comprehensive multiphase (CMP) NMR combines all electronics and hardware (magic angle spinning [MAS], gradients, high power Radio Frequency (RF) handling, lock, susceptibility matching) into a universal probe that permits a comprehensive study of all phases (i.e., liquid, gel‐like, semisolid, and solid), in intact samples. When applied in vivo, it provides unique insight into the wide array of bonds in a living system from the most mobile liquids (blood, fluids) through gels (muscle, tissues) to the most rigid (exoskeleton, shell). In this tutorial, the practical aspects of in vivo CMP NMR are discussed including: handling the organisms, rotor preparation, sample spinning, water suppression, editing experiments, and finishes with a brief look at the potential of other heteronuclei (2H, 15N, 19F, 31P) for in vivo research. The tutorial is aimed as a general resource for researchers interested in developing and applying MAS‐based approaches to living organisms. Although the focus here is CMP NMR, many of the approaches can be adapted (or directly applied) using conventional high‐resolution magic angle spinning, and in some cases, even standard solid‐state NMR probes.

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“…magna and H . azteca are provided elsewhere . Specifically for NMR studies, the organisms can be enriched by replacing their normal food source (algae) withC‐enriched algae.…”

Section: Sample Preperation Handling and Spinningmentioning

confidence: 99%

Section: Sample Preperation Handling and Spinningmentioning

confidence: 99%

Section: Sample Preperation Handling and Spinningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vivo comprehensive multiphase NMR

Mobarhan

Soong

Lane

et al. 2019

Magnetic Reson in Chemistry

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“…NMR spectroscopy is becoming a powerful tool for environmental and biomedical research . NMR is the only nondestructive technique capable of providing rich molecular information on even the most disordered and complex systems . With the aid of proper experiments and reference databases, NMR can deconvolute complex mixtures without physical separation, making it an ideal tool for nontargeted screening .…”

Section: Introductionmentioning

confidence: 99%

Flow‐based in vivo NMR spectroscopy of small aquatic organisms

Soong

Mobarhan

Tabatabaei

et al. 2019

Magnetic Reson in Chemistry

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NMR applied to living organisms is arguably the ultimate tool for understanding environmental stress responses and can provide desperately needed information on toxic mechanisms, synergistic effects, sublethal impacts, recovery, and biotransformation of xenobiotics. To perform in vivo NMR spectroscopy, a flow cell system is required to deliver oxygen and food to the organisms while maintaining optimal line shape for NMR spectroscopy. In this tutorial, two such flow cell systems and their constructions are discussed: (a) a single pump high‐volume flow cell design is simple to build and ideal for organisms that do not require feeding (i.e., eggs) and (b) a more advanced low‐volume double pump flow cell design that permits feeding, maintains optimal water height for water suppression, improves locking and shimming, and uses only a small recirculating volume, thus reducing the amount of xenobiotic required for testing. In addition, key experimental aspects including isotopic enrichment, water suppression, and 2D experiments for both 13C enriched and natural abundance organisms are discussed.

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