2011
DOI: 10.1371/journal.pone.0029040
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In Vivo Tracking of Human Neural Stem Cells with 19F Magnetic Resonance Imaging

Abstract: BackgroundMagnetic resonance imaging (MRI) is a promising tool for monitoring stem cell-based therapy. Conventionally, cells loaded with ironoxide nanoparticles appear hypointense on MR images. However, the contrast generated by ironoxide labeled cells is neither specific due to ambiguous background nor quantitative. A strategy to overcome these drawbacks is 19F MRI of cells labeled with perfluorocarbons. We show here for the first time that human neural stem cells (NSCs), a promising candidate for clinical tr… Show more

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Cited by 113 publications
(149 citation statements)
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“…26,29 Others have also shown that Cell Sense does not impact the phenotype or functioning of dendritic cells 12 or differentiation in other stem cell types. 22,23 We have also successfully demonstrated in vivo detectability of hMSC labeled with the only fluorine-based contrast agent that has been FDA-approved for cellular imaging, using the 3D-bSSFP imaging sequence. This is the first study to monitor the fate of hMSC using 19 F MRI.…”
Section: Discussionmentioning
confidence: 97%
See 1 more Smart Citation
“…26,29 Others have also shown that Cell Sense does not impact the phenotype or functioning of dendritic cells 12 or differentiation in other stem cell types. 22,23 We have also successfully demonstrated in vivo detectability of hMSC labeled with the only fluorine-based contrast agent that has been FDA-approved for cellular imaging, using the 3D-bSSFP imaging sequence. This is the first study to monitor the fate of hMSC using 19 F MRI.…”
Section: Discussionmentioning
confidence: 97%
“…Boehm-Sturm et al first demonstrated that the fluorine signal changes by less than 20% from day 2 and day 6 after the implantation of 150,000 human neural stem cells (NSCs) directly into the striatum in healthy mice. 23 Ruiz-Cabello et al imaged mouse NSCs implanted into normal mouse striatum and showed that the 19 F signal remained constant for 1 week post-implantation and was diminished but still visible for up to 2 weeks. 41 Histopathology showed that the labeled cells remained viable and that labeled cells had migrated from the site of implantation into the brain parenchyma.…”
Section: Cell Detection With Bssfpmentioning
confidence: 99%
“…25,26 Other investigators have also reported on the ability to ex vivo label other potential cell therapies with 19 F, including murine 30 and human neural stem cells, 31 human CD34 C hematopoietic stem cells (HSCs) and mouse bone marrow derived HSCs as well as murine 32 and human 33 19 F-MRI without apparent toxicity. 34 About 10 million DCs were injected intradermally into quadriceps and patients were imaged for about 9.5 min.…”
mentioning
confidence: 99%
“…A recent study using human neural stem cells (NSCs) efficiently labeled with 19 -perfluoropolyether (PFPE) with little effects on viability or proliferation and differentiation capacities, has shown for the first time that 19 F-MRI can be utilized for tracking human NSCs in brain implantation studies, which ultimately could aim for restoring loss of function after acute and neurodegenerative disorders [40]. This study suggests that stem cells (SCs) involved in the initiation and/ or propagation of tumor cells can also be labeled and monitored by F-MRI, which is tremendously useful for better understanding the general oncogenesis/carcinogenesis process (e.g.…”
Section: Magnetic Resonance Imaging (Mri)mentioning
confidence: 99%