In vivo volumetric imaging by crosstalk-free full-field OCT

Stremplewski, Patrycjusz; Auksorius, Egidijus; Wnuk, Paweł; Kozoń, Łukasz; Garstecki, Piotr; Wojtkowski, Maciej

doi:10.1364/optica.6.000608

Cited by 62 publications

(40 citation statements)

References 45 publications

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“…Cornea is a good target for FD-FF-OCT due to its low scattering and high transparency that makes the confocal pinhole redundant. Crosstalk, appearing due to scattering, should also be less of an issue compared to skin [39] or retina [41] in vivo imaging with FD-FF-OCT. However, we could not determine how much of the crosstalk was suppressed by using DM in this work due to the laser safety constraints that made it impossible to acquire images with spatially coherent illumination at full power.…”

Section: Discussionmentioning

confidence: 99%

“…Such incoherent illumination effectively broadens the laser spot on the retina to the acceptable MPE intensity levels. We have previously used DM to remove crosstalk in FD-FF-OCT images of skin [39] and retina [41]. In corneal imaging, however, crosstalk is less of an issue due to the low corneal scattering, and therefore, the DM is primarily used to circumvent the laser safety problem.…”

Section: Introductionmentioning

confidence: 99%

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In vivo imaging of the human cornea with high-speed and high-resolution Fourier-domain full-field optical coherence tomography

Auksorius

Borycki

Stremplewski

et al. 2020

Biomed. Opt. Express

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Corneal evaluation in ophthalmology necessitates cellular-resolution and fast imaging techniques allowing accurate diagnoses. Currently, the fastest volumetric imaging technique is Fourier-domain full-field optical coherence tomography (FD-FF-OCT) that uses a fast camera and a rapidly tunable laser source. Here, we demonstrate high-resolution, highspeed, non-contact corneal volumetric imaging in vivo with FD-FF-OCT that can acquire a single 3D volume with a voxel rate of 7.8 GHz. The spatial coherence of the laser source was suppressed to prevent it from focusing to a spot on the retina, and therefore, exceeding the maximum permissible exposure (MPE). Inherently volumetric nature of FD-FF-OCT data enabled flattening of curved corneal layers. Acquired FD-FF-OCT images revealed corneal cellular structures, such as epithelium, stroma and endothelium, as well as subbasal and midstromal nerves.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vivo imaging of the human cornea with high-speed and high-resolution Fourier-domain full-field optical coherence tomography

Auksorius

Borycki

Stremplewski

et al. 2020

Biomed. Opt. Express

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“…It has been shown that the lateral resolution of images acquired in OCT with line-field or full-field illumination of the sample is degraded when spatially-coherent light rather than spatially-incoherent light is used [5,37,38]. This is the result of a phenomenon referred to as optical crosstalk [39].…”

Section: Lateral Resolutionmentioning

confidence: 99%

“…In addition, movements of the sample may generate random phase variations whose averaging over the interferometric image acquisition time (⇠ 10 µs) would reduce the e ect of crosstalk. This concept has been applied in full-field FD-OCT using a fast deformable membrane that introduces random phase illumination to produce crosstalk free images of human skin [38].…”

Section: Lateral Resolutionmentioning

confidence: 99%

Mirau-based line-field confocal optical coherence tomography

Dubois¹,

Xue²,

Levecq³

et al. 2020

Opt. Express

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Line-field confocal optical coherence tomography (LC-OCT) is an imaging technique in which A-scans are acquired in parallel through line illumination with a broadband laser and line detection with a line-scan camera. B-scan imaging at high spatial resolution is achieved by dynamic focusing in a Linnik interferometer. This paper presents an LC-OCT device based on a custom-designed Mirau interferometer that o ers similar spatial resolution and detection sensitivity. The device has the advantage of being more compact and lighter. In vivo imaging of human skin with a resolution of 1.3 µm ⇥ 1.1 µm (lateral ⇥ axial) is demonstrated over a field of 0.9 mm ⇥ 0.4 mm (lateral ⇥ axial) at 12 frames per second.

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“…In this manuscript, we present a general idea of novel Spatio-Temporal Optical Coherence (STOC) phase manipulation [4][5][6]. STOC enables to reduce the contribution from multiply scattered photons by disentangling them from ballistic photons in the interferometric measurement scheme.…”

mentioning

confidence: 99%

Spatio-Temporal Optical Coherence Imaging – a new tool for in vivo microscopy

et al. 2019

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Optical Coherence Imaging (OCI) including Optical Coherence Tomography (OCT) and Optical Coherence Microscopy (OCM) uses interferometric detection to generate high-resolution volumetric images of the sample at high speeds. Such capabilities are significant for in vivo imaging, including ophthalmology, brain, intravascular imaging, as well as endoscopic examination. Instrumentation and software development allowed to create many clinical instruments. Nevertheless, most of OCI setups scan the incident light laterally. Hence, OCI can be further extended by wide-field illumination and detection. This approach, however, is very susceptible to the so-called crosstalk-generated noise. Here, we describe our novel approach to overcome this issue with spatio-temporal optical coherence manipulation (STOC), which employs spatial phase modulation of the incident light. Full Text: PDF ReferencesL. Wang, P. P. Ho, C. Liu, G. Zhang, and R. R. Alfano, "Ballistic 2-D Imaging Through Scattering Walls Using an Ultrafast Optical Kerr Gate", Science 253, 769-771 (1991). CrossRef D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and et al., "Optical coherence tomography", Science 254, 1178-1181 (1991). CrossRef J. A. Izatt, E. A. Swanson, J. G. Fujimoto, M. R. Hee, and G. M. Owen, "Optical coherence microscopy in scattering media", Opt. Lett. 19, 590-592 (1994). CrossRef D. Borycki, M. Nowakowski, and M. Wojtkowski, "Control of the optical field coherence by spatiotemporal light modulation", Opt. Lett. 38, 4817-4820 (2013). CrossRef D. Borycki, M. Hamkalo, M. Nowakowski, M. Szkulmowski, and M. Wojtkowski, "Spatiotemporal optical coherence (STOC) manipulation suppresses coherent cross-talk in full-field swept-source optical coherence tomography", Biomed. Opt. Express 10, 2032-2054 (2019). CrossRef P. Stremplewski, E. Auksorius, P. Wnuk, L. Kozon, P. Garstecki, and M. Wojtkowski, "In vivo volumetric imaging by crosstalk-free full-field OCT", Optica 6, 608-617 (2019). CrossRef L. Vabre, A. Dubois, and A. C. Boccara, "Thermal-light full-field optical coherence tomography", Opt. Lett. 27, 530-532 (2002). CrossRef M. Laubscher, M. Ducros, B. Karamata, T. Lasser, and R. Salathé, "Video-rate three-dimensional optical coherence tomography", Opt. Express 10, 429-435 (2002). CrossRef Dubois and A. C. Boccara, Full-Field Optical Coherence Tomography, (Springer Berlin Heidelberg, Berlin, Heidelberg, 2008), pp. 565-591. CrossRef O. Thouvenin, K. Grieve, P. Xiao, C. Apelian, and A. C. Boccara, "En face coherence microscopy [Invited]", Biomedical Opt. Express 8, 622-639 (2017). CrossRef F. Fercher, C. K. Hitzenberger, M. Sticker, E. Moreno-Barriuso, R. Leitgeb, W. Drexler, and H. Sattmann, "A thermal light source technique for optical coherence tomography", Optics Commun. 185, 57-64 (2000). CrossRef R. A. Leitgeb, "En face optical coherence tomography: a technology review [Invited]", Biomed Opt Express 10, 2177-2201 (2019). CrossRef J. Fujimoto and W. Drexler, Introduction to Optical Coherence Tomography, (Springer, Berlin, Heidelberg, 2008), pp. 1-45. CrossRef J. A. Izatt, M. A. Choma, and A.-H. Dhalla, Theory of Optical Coherence Tomography, (Springer International Publishing, Cham, 2015), pp. 65-94. CrossRef

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In vivo volumetric imaging by crosstalk-free full-field OCT

Cited by 62 publications

References 45 publications

In vivo imaging of the human cornea with high-speed and high-resolution Fourier-domain full-field optical coherence tomography

In vivo imaging of the human cornea with high-speed and high-resolution Fourier-domain full-field optical coherence tomography

Mirau-based line-field confocal optical coherence tomography

Spatio-Temporal Optical Coherence Imaging – a new tool for in vivo microscopy

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