IncA/C plasmids conferring high azithromycin resistance in vibrio cholerae

Wang, Ruibai; Liu, Haican; Zhao, Xiuqin; Li, Jie; Wan, Kanglin

doi:10.1016/j.ijantimicag.2017.09.009

Cited by 21 publications

(26 citation statements)

References 17 publications

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“…V. cholerae O1 El Tor strain C6706 was isolated in 1991 from Peru [16]. The IncA/C 2 plasmid pVC211 was identified in a toxigenic O139 serogroup strain VC211 isolated in 2003, Guangdong province, China [6]. The pVC211 plasmid was transferred into C6706 by conjugation and the strain CV2 was constructed.…”

Section: Methodsmentioning

confidence: 99%

“…For example, the pNDM-1_Dok01, pNDM102337, pNDM10469, pNDM10505, pNDMCFuy, pNDM-KN and pNDM-US plasmids of Escherichia coli and Klebsiella pneumoniae . The pVC211 plasmid we isolated in Vibrio cholerae has 16 antibiotic resistance related genes, including five macrolide resistant genes involving in three mechanisms [6]. The IncA/C plasmid made 99% of Vibrio cholerae O139 strains resistant to more than three antibiotics in which 47% were resistant to eight antibiotics [7].…”

Section: Introductionmentioning

confidence: 99%

“…Though the role of IncA/C plasmids in drug resistance is well elucidated, the role of the dcm genes on A/C 2 plasmids remains largely unexplored. In this study, the IncA/C plasmid pVC211 (148,456bp) [6], was conjugated into V. cholerae strain C6706. Sequencing of genomic DNA provided a view of the change in the methylation patterns of the host that were induced by this plasmid.…”

Section: Introductionmentioning

confidence: 99%

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Large scale changes in host methylation patterns induced by IncA/C plasmid transformation inVibrio cholerae

Wang

Wan

2018

Preprint

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19DNA methylation is a central epigenetic modification and has diverse biological 20 functions in eukaryotic and prokaryotic organisms alike. The IncA/C plasmid 21 genomes are approximately 150kb in length and harbour three methylase genes, two 22 of which demonstrate cytosine specificity. Transformation of the Vibrio cholerae 23 strain C6706 with the IncA/C plasmid pVC211 resulted in a significant relabelling of 24 the methylation patterns on the host chromosomes. The new methylation patterns 25 induced by transformation with IncA/C plasmid were accepted by the restriction 26 enzymes of the host's restriction modification (RM) system. These data uncover a 27 novel mechanism by which plasmids can be compatible with a host's RM system and 28 suggest a possible reason that plasmids of the IncA/C family are broad-host-range. 30Author summary: 31 Antibiotic resistance of bacteria is a growing serious problem worldwidely and 32 the horizontal transfer of multi-drug resistance genes mediated by plasmids within 33 and between species of bacteria is the main reason. In the researches of multi-drug 34 resistance of Vibrio cholerae, I have isolated several IncA/C plasmids. What 35 impressed me most is their ability to accumulate the resistant genes. Moreover, they 36 can transfer with high frequency and are stable in several bacterial species. There are 37 at least three Tra regions on the IncA/C plasmid which containing components of the 38 Type 4 Secretion System and are important for conjugative transfer of plasmids. So 39 the horizontal transfer ability of IncA/C plasmids is reasonable. There are three 40 methylase genes on the small genome of IncA/C plasmids, which demonstrate 41 cytosine specificity and are seldom in bacteria. Their modification target and roles are 42interesting. Here, we analysed the methylation profiles of the host V. choerae induced 43 by the plasmid pVC211 and found that they were completely changed. In addition to 44 replicons, this may be a novel mechanism that plasmid cross the barrier of the host's 45 RM system and become broad-host range. Changing the activity of methylase in 46 IncA/C plasmids may be a new way to affect the stability of IncA/C plasmids to 47 51 plasmid-incompatible groups (Inc) and mediate the dissemination of multi-drug 52 resistance in bacteria [1,2]. Both of the plasmid pIP1202, which has high resistance to 53 at least eight antibiotics isolated from Yersinia pestis IP275 in 1995 [3], and the 54 plasmid of NDM-1 super-resistant bacteria in India, Bangladesh, Pakistan, Britain and 55 the United States in August 2010 [4] are IncA/C family plasmids and have aroused 56 great concern in public health and bioterrorism. IncA/C plasmids have great ability to 57 accumulate antibiotic resistant genes. There are many resistance genes of rifampicin, 58 erythromycin, streptomycin, chloramphenicol, sulfonamides and disinfectants 59 routinely harbored on the plasmids [5], as well as a variety of beta-lactamase genes. 60 For example, the pNDM-1_Dok01, pNDM102337, pND...

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Large scale changes in host methylation patterns induced by IncA/C plasmid transformation inVibrio cholerae

Wang

Wan

2018

Preprint

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“…pEI-MS-17-156-1 contains resistance genes floR , sul2 , and tetD , and this plasmid is nearly identical (almost 100% ANI) to previously sequenced plasmids isolated from a wide variety of Gram-negative bacteria, including Vibrio alginolyticus strain Vb0506 (pVb0506) ( 13 ), Klebsiella pneumoniae strain K-109-R plasmid ( 14 ), Photobacterium damselae subsp. piscicida (pP91278) ( 15 ), Vibrio cholerae strain ICDC-1447 (pVC1447) ( 16 ), Salmonella enterica subsp. enterica serovar Senftenberg strain SRC119 (pSRC119-A/C) ( 17 ), and Escherichia coli strain PG010208 (pPG010208) ( 18 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Multidrug-Resistant Edwardsiella ictaluri Strain MS-17-156

et al. 2018

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Edwardsiella ictaluri is a significant pathogen of cultured fish, particularly channel catfish. Here, we present the complete genome sequence of a multidrug-resistant E. ictaluri strain, MS-17-156, isolated from diseased channel catfish. The genome sequence of this multidrug-resistant strain is expected to help us understand the molecular mechanism of antibiotic resistance in this important pathogen.

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“…A single dose azithromycin is typically preferred in the area where reduced susceptibilities to fluoroquinolones are common. However, azithromycin resistance emerged in the 1990's and became more common and concerning due to several resistance mechanisms including plasmid mediation and macrolide resistant proteins [ 24 ].…”

Section: Managementmentioning

confidence: 99%

Current views and challenges on clinical cholera

et al. 2017

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Cholera, an acute diarrheal infection has become a major global threat. Vibrio cholerae the causative agent of cholera has been responsible for six previous pandemics since 1817 that spanned four continents and Australia with the seventh pandemic ongoing since 1961. Two serogroups of V. cholerae O1 and O139 have the ability to secrete the enterotoxin with potential to cause epidemics. The prior six pandemics were caused by the classical biotype of the O1 serogroup. However, the emergence of the El Tor biotype and subsequent variants of El Tor with classical traits are the main isolates in the seventh pandemic.Cholera outbreaks have increased among vulnerable communities affected by war, earthquakes, conflicts and famines. Annually, 2.9 million cases of cholera occur globally in 69 endemic countries with 95,000 deaths. Early detection followed by prompt fluid and electrolyte replacement can reduce the case fatality ratio significantly. Improvements in water systems, sanitation and hygiene have effectively eliminated the transmission of cholera in high-income countries and reduced transmission in some developing nations. However, an estimated 1.8 billion are still at risk for cholera due to lack of potable water, inadequate sanitation and hygiene. Interventions focusing on hygiene in conjunction with proper disposal and treatment of sewage and provision of safe drinking water are likely to be effective in preventing the recurrence of cholera. Lastly, the use of current oral vaccines in endemic settings in combination with WASH interventions may be an effective approach to prevent and reduce the spread of cholera infection.

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IncA/C plasmids conferring high azithromycin resistance in vibrio cholerae

Cited by 21 publications

References 17 publications

Large scale changes in host methylation patterns induced by IncA/C plasmid transformation inVibrio cholerae

Large scale changes in host methylation patterns induced by IncA/C plasmid transformation inVibrio cholerae

Complete Genome Sequence of Multidrug-Resistant Edwardsiella ictaluri Strain MS-17-156

Current views and challenges on clinical cholera

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