Incidence of Arcobacter spp. in fresh seafood from retail markets in Mumbai, India

Rathlavath, Srinu; Mishra, Saurabh S.; Kumar, Sanath; Nayak, Binaya Bhusan

doi:10.1007/s13213-015-1092-3

Cited by 10 publications

(10 citation statements)

References 40 publications

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“…The incidence of Arcobacter sp. observed in this study was in accordance with previous reports which demonstrated a prevalence of 21·33% (Patyal et al ), 24% (Laishram et al ), 25% (Rathlavath et al ) and 32·8% (Rathlavath et al ) from sea food (fish and shellfish) in India. Unhygienic practices and multiple handling of fish in markets along with improper drainage and deposits of fish wastes could be responsible for cross contamination and high incidence of arcobacters in market fish.…”

Section: Resultssupporting

confidence: 93%

“…Currently, this genus comprises 27 recognized species isolated from different environments and hosts (Levican et al 2015;Whiteduck-L eveill ee et al 2015;P erez-Cataluña et al 2018), of which Arcobacter butzleri, A. cryaerophilus, A. cibarus, A. skirrowii and A. mytili are considered as potential human pathogens (Ho et al 2006;Collado and Figueras 2011;Levican et al 2015). Pathogenic arcobacters have been associated with gastrointestinal and extra-gastrointestinal diseases ranging from diarrhoea to bacteraemia (Figueras et al 2014) and hence are increasingly being recognized as emerging sea foodand water-borne human pathogens (Fera et al 2004;Collado and Figueras, 2011;Patyal et al 2011;Rathlavath et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Occurrence, virulence gene and antimicrobial susceptibility profiles of Arcobacter sp . isolated from catla ( Catla catla ) in India

Nelapati

Tumati

Thirtham

et al. 2020

Lett Appl Microbiol

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In the present study, a total of 100 catla (Catla catla—major South Asian carp, local name botcha) collected from local fish markets and aquaculture ponds were subjected for isolation and characterization of Arcobacter sp. In all, 21 Arcobacter sp. were isolated, of which 18 (85·7%) were Arcobacter butzleri and three (14%) were A. cryoaerophilus as identified by multiplex PCR. All 18 A. butzleri isolates were positive for mviN, ciaB and tlyA virulence genes, three of A. cryoaerophilus isolates carried mviN gene and none of the isolates were positive for cadF, irgA, cj1349, hecA and hecB genes. All isolates (n = 21) were resistant to penicillin (100%). Meanwhile, 71·43, 23·81, 23·81, 14·29 and 9·52% of the isolates showed resistance towards vancomycin, nalidixic acid, erythromycin, cefixime and kanamycin, respectively. Multidrug resistance was observed in 23·81% of the Arcobacter sp. isolates and none of the isolates were positive for any of the extended spectrum beta‐lactamases either by phenotypic or by molecular identification genes (blaOXA, blaSHV, blaTEM, blaCTX‐M1, blaCTX‐M2 and blaCTX‐M9 groups). The results emphasize the need to implement specific control procedures to reduce the use of antibiotics in aquaculture particularly the ones which are very important in human medicine. Significance and Impact of the Study Arcobacter species are emerging food‐ and water‐borne human pathogens. In this study, Arcobacter butzleri was predominant in fish compared to A. cryoaerohilus and A. skirrowii. Higher incidence of arcobacters in fish market samples suggests cross contamination and unhygienic handling of fish in markets. Virulence genes profile and antibiotics resistance of the Arcobacter sp. isolated in current study indicate pathogenic potential of Arcobacter sp. to humans. Occurrence of multidrug‐resistant Arcobacter sp. in fish is a major concern in food safety. To our knowledge, this is the first report of Arcobacter sp. from freshwater fish, catla (Catla catla) in India.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Occurrence, virulence gene and antimicrobial susceptibility profiles of Arcobacter sp . isolated from catla ( Catla catla ) in India

Nelapati

Tumati

Thirtham

et al. 2020

Lett Appl Microbiol

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“…; Rathlavath et al . ). The isolation of A. skirrowii from fish and A. mytili from water samples has been previously reported from India (Laishram et al .…”

Section: Resultsmentioning

confidence: 97%

“…; Rathlavath et al . ). In this study, A. defluvii was isolated from a sample of ribbon fish ( Trichiurus lepturus ).…”

Section: Resultsmentioning

confidence: 99%

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Comparative isolation and genetic diversity ofArcobactersp. from fish and the coastal environment

Rathlavath

Kumar

Nayak

2017

Lett Appl Microbiol

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Arcobacter sp. are emerging food- and water-borne human pathogens. In this study, comparison of two selective media suggested Arcobacter blood agar to be more efficient in yielding Arcobacter sp. from seafood. Furthermore, the isolation of Arcobacter sp. such as Arcobacter butzleri, A. skirrowii, A. mytili and A. defluvii from seafood suggests diverse sources of contamination of seafood by Arcobacter sp. Analysis of enterobacterial repetitive intergenic consensus sequence-PCR patterns of A. butzleri showed high genetic diversity and lack of clonality among the isolates. Arcobacter contamination of seafood is an emerging issue both from seafood safety and seafood trade point of view.

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Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

Disli,

Hizlisoy,

Gungor

et al. 2024

Int Microbiol

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Aliarcobacter spp. have has been isolated from numerous food products at retail and from animal carcasses and feces at slaughter. The objectives of this study were: i) to isolate Aliarcobacter species (Aliarcobacter butzleri, Aliarcobacter skirrowii, Aliarcobacter cryaerophilus) from different slaughterhouses' samples, ii) to detect genetic diversity, antibiotic resistance, bio lm ability and putative virulence gene pro les of the isolates. The antibiotic susceptibility to antimicrobials was examined using the Kirby-Bauer disc diffusion method. A molecular investigation of antibiotic resistance and virulence factors was also conducted using polymerase chain reaction (PCR).A total of 22 (14.6%) Aliarcobacter spp. isolates were obtained, with varying levels of antibiotic resistance observed. The genes tetO, tetW, and gyrA were detected in 0%, 31.8%, and 27.2% of the isolates, respectively. All isolates were resistant to ampicillin, rifampin, and erythromycin, while tetracycline was found to be the most effective antibiotic, with 81.8% of the isolates showing susceptibility to it. In terms of virulence factors, all isolates (100%) harbored more than one of the nine putative virulence genes tested, with 18.1% of isolates carrying more than three.Regarding bio lm formation, 7 (31.8%) and 4 (18.1%) isolates were found to form strong and moderate bio lms, respectively, while one (4.5%) isolate was classi ed as a weak bio lm producer. ERIC-PCR band patterns suggested that the isolated Aliarcobacter spp. from slaughterhouses had different sources of contamination. These ndings highlight the potential risk posed by pathogenic and multidrug-resistant Aliarcobacter spp. in food, and the need for control measures throughout the food chain to prevent the spread of these strains. The results indicate that foods of animal origin and cattle slaughterhouses are signi cant sources of antimicrobial resistant Aliarcobacters.

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Incidence of Arcobacter spp. in fresh seafood from retail markets in Mumbai, India

Cited by 10 publications

References 40 publications

Occurrence, virulence gene and antimicrobial susceptibility profiles of Arcobacter sp . isolated from catla ( Catla catla ) in India

Occurrence, virulence gene and antimicrobial susceptibility profiles of Arcobacter sp . isolated from catla ( Catla catla ) in India

Comparative isolation and genetic diversity ofArcobactersp. from fish and the coastal environment

Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

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