Incidence of enterovirus in patients with acute gastroenteritis

Pérez-Martínez, Zulema; Álvarez-Argüelles, Marta Elena; Rojo‐Alba, Susana; Castelló-Abiétar, Cristian; Boga, José Antonio; Morilla-Morilla, A.; Vivanco-Allende, Ana; Rodríguez, Julián; Alonso-Álvarez, M. A.; Melón, Santiago

doi:10.1007/s10096-021-04275-6

Cited by 5 publications

(6 citation statements)

References 23 publications

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“…Among 101 EV-positive cases, co-infection with other common diarrheal viruses was found in 31.7% (32 out of 101) of cases, a finding similar to the studies conducted in Switzerland (33%) ( Gosert et al, 2018 ) and Spain (39.7%) ( Perez-Martinez et al, 2021 ). Notably, single infection by EV was as high as 68.3% in this study, suggesting a causative effect in AGE patients.…”

Section: Discussionsupporting

confidence: 83%

“…These findings suggest a potential influence of the control measures for the COVID-19 pandemic on a decline in the prevalence of EV infection and genotype diversity in pediatric patients with AGE. Among 101 EV-positive cases, co-infection with other common diarrheal viruses was found in 31.7% (32 out of 101) of cases, a finding similar to the studies conducted in Switzerland (33%) (Gosert et al, 2018) and Spain (39.7%) (Perez-Martinez et al, 2021). Notably, single infection by EV was as high as 68.3% in this study, suggesting a causative effect in AGE patients.…”

Section: Cv-a9supporting

confidence: 87%

“…It should be noted that norovirus was more frequently detected in patients with AGE in Thailand during the same period ( Jampanil et al, 2021 ; Wei et al, 2021 ; Phengma et al, 2022 ; Hogben et al, 2023 ; Yodmeeklin et al, 2023 ). Similarly, adenovirus was the most predominant virus detected in AGE patients in Spain from 2013 to 2017, and it was found to be the most common co-infection virus detected in EV-positive cases ( Perez-Martinez et al, 2021 ). Co-infection is frequently observed among AGE patients although the rates of co-infection vary depending on the country and the number of pathogens detected ( Makimaa et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…A number of recent studies have revealed an increasing prevalence of EV in AGE patients worldwide, ranging from 5.3 to 26.3% ( Phan et al, 2005 ; Chitambar et al, 2012 ; Kumthip et al, 2017 ; Gosert et al, 2018 ; Pham et al, 2018 ; Machado et al, 2020 ; Perez-Martinez et al, 2021 ; Gelaw and Liebert, 2022 ). The studies conducted in Switzerland and Ethiopia demonstrated that EV was one of the three most commonly identified pathogens in patients with AGE ( Gosert et al, 2018 ; Gelaw and Liebert, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Enterovirus genotype diversity with emergence of coxsackievirus A2 circulating in pediatric patients with acute gastroenteritis in Thailand, 2019–2022

Xie,

Khamrin,

Jampanil

et al. 2024

Front. Microbiol.

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IntroductionEnteroviruses (EVs) are recognized as potential causative agents of acute gastroenteritis (AGE) in children worldwide. This study aimed to investigate the epidemiology and molecular characteristics of EV infection in children admitted to hospitals with AGE in Chiang Mai, Thailand from 2019 to 2022.MethodsA total of 1,148 fecal samples collected from patients with AGE were screened for the presence of EV using RT-PCR. The prevalence, co-infection with common diarrheal viruses, and seasonal pattern of EV were examined. The genotypes of EV were identified based on the VP1 sequence and phylogenetic analysis.ResultsThe overall prevalence of EV in AGE patients was 8.8% (101/1,148). After the COVID-19 outbreak in 2019, a significant decrease in the EV infection rate and genotype diversity was observed (p < 0.05). EV infection alone was observed in 68.3% (69/101) of cases while co-infection with other enteric viruses was 31.7% (32/101). The seasonal pattern of EV infection showed a peak prevalence during the rainy season. EV species A was the most prevalent (37.5%), followed by species B (32.3%), species C (29.2%), and species D (1.0%). Twenty-five genotypes of EV were identified with the most predominant of the coxsackievirus A2 (CV-A2) (13.5%), CV-B2 (7.3%) and CV-A24 (5.2%).ConclusionOur data demonstrate a significant decrease in the prevalence and diversity of EV circulating in AGE patients during the COVID-19 pandemic and highlight the emergence of CV-A2 during this study period. These findings contribute to a better understanding of the molecular epidemiology and diversity of EV in patients with AGE and provide useful information for further investigation into the potential association between specific EV genotypes and AGE in future studies.

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Section: Discussionsupporting

confidence: 83%

Section: Cv-a9supporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enterovirus genotype diversity with emergence of coxsackievirus A2 circulating in pediatric patients with acute gastroenteritis in Thailand, 2019–2022

Xie,

Khamrin,

Jampanil

et al. 2024

Front. Microbiol.

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“…Enteric viruses, such as enterovirus (EV) and rotavirus A (RVA), are excreted in high concentrations in the feces of infected individuals and are considered important causes of diarrheal disease and significant source of water contamination (Justino et al 2019, Machado et al 2020, Pérez-Martínez et al 2021, Gholipour et al 2022.…”

Section: _________________ Corresponding Editor: María S Morales Cova...mentioning

confidence: 99%

Two-year monitoring of enterovirus and rotavirus A in recreational freshwater from an island region, Pará State, northern Brazil

Alves

Teixeira

Deus

et al. 2022

Lat. Am. J. Aquat. Res.

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Enteric viruses are major causes of waterborne diseases and are present in large quantities in the stools of infected individuals. Its viability in the environment lasts for months, favoring the contamination of water used for consumption and recreation. The study aimed to monitor monthly the circulation of enterovirus (EV) and group A rotavirus (RVA) in recreational freshwater from an island region used as a bathhouse in northern Brazil, from January 2012 to December 2013. The viral RNA was obtained using guanidine isothiocyanate/silica after viral concentration by adsorption-elution method. The molecular detection was carried out by semi (EV) and nested-PCR (RVA) and the amplicons were sequenced on automated sequencer. At least one of these viruses was detected on 40.4% (42/104) of the samples. RVA was the most frequent (n = 32; 30.8%) when compared to EV (n = 20; 19.2%). Co-circulation between both was identified in 9.6% (n = 10). The highest viral positivity was found in SP02 (46.1%). The highest viral positivity was observed during high tides (57.7%; 60/104). Most EV samples were characterized as coxsackievirus (CV) A5 (85.7%, 12/14) and others as Sabin 1 poliovirus (14.2%, 2/14). The RVA positive samples were genotyped as G2, G3, G9, G12, P[8], P[4], and P[6]. These viruses were detected in 35.6% (37/104) of the samples with an acceptable concentration of fecal coliform bacteria. These results demonstrate the contamination of surface water intended for recreation by enteric viruses of Public Health concern even when bacterial indicators are within the tolerated limit, a factor that confirms the need for public policies aimed the sewage treatment before its release into water bodies.

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Epidemiology and molecular detection of human adenovirus and non-polio enterovirus in fecal samples of children with acute gastroenteritis: A five-year surveillance in northern Brazil

Alves,

Teixeira,

Siqueira

et al. 2024

PLoS ONE

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Acute gastroenteritis (AGE) is a common pediatric infection that remains a significant cause of childhood morbidity and mortality worldwide, especially in low-income regions. Thus, the objective of this study was to detect human adenovirus (HAdV) and non-polio enterovirus (NPEV) in fecal samples from the Gastroenteritis Surveillance Network, and to identify circulating strains by nucleotide sequencing. A total of 801 fecal samples were tested using qPCR/RT-qPCR, and 657 (82.0%) were inoculated into HEp-2C and RD cell lines. The HAdV and NPEV positivity rates obtained using qPCR/RT-qPCR were 31.7% (254/801) and 10.5% (84/801), respectively, with 5.4% (43/801) co-detection. Cytopathic effect was observed in 9.6% (63/657) of patients, 2.7% (18/657) associated with HAdV, and 6.2% (41/657) associated with NPEV after testing by ICC-PCR. A comparison of the two methodologies demonstrated an agreement of 93.5% for EVNP and 64.4% for HAdV. These two viruses were detected throughout the study period, with HAdV positivity rates ranging from 41% in Amapá to 18% in Pará. The NEPV varied from 18% in Pará/Rondônia to 3% in Acre. The most affected age group was over 60 months for both HAdV and NPEV. Samples previously positive for rotavirus and norovirus, which did not show a major difference in the presence or absence of diarrhea, fever, and vomiting, were excluded from the clinical analyses of these two viruses. These viruses circulated over five years, with a few months of absence, mainly during the months corresponding to the waves of SARS-CoV-2 infection in Brazil. Five HAdV species were identified (A, B, C, D, and F), with a greater predominance of HAdV-F41 (56.5%) followed by HAdV-C (15.2%). Three NPEV species (A, B, and C) were detected, with serotypes E14 (19.3%) and CVA-24 (16.1%) being the most prevalent. The present study revealed a high diversity of NPEV and HAdV types circulating in children with AGE symptoms in the northern region of Brazil.

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Incidence of enterovirus in patients with acute gastroenteritis

Cited by 5 publications

References 23 publications

Enterovirus genotype diversity with emergence of coxsackievirus A2 circulating in pediatric patients with acute gastroenteritis in Thailand, 2019–2022

Enterovirus genotype diversity with emergence of coxsackievirus A2 circulating in pediatric patients with acute gastroenteritis in Thailand, 2019–2022

Two-year monitoring of enterovirus and rotavirus A in recreational freshwater from an island region, Pará State, northern Brazil

Epidemiology and molecular detection of human adenovirus and non-polio enterovirus in fecal samples of children with acute gastroenteritis: A five-year surveillance in northern Brazil

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