Incidence of <i>Prunus necrotic ring spot virus</i> on <i>Malus domestica</i> in India

Chandel, Vanita; Rana, Tanuja; Handa, Anil; Thakur, Priyanka; Hallan, Vipin; Zaidi, A. A.

doi:10.1111/j.1439-0434.2007.01361.x

Cited by 23 publications

(12 citation statements)

References 9 publications

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“…Results of DAS-ELISA tests conducted on the test samples presented in (Polak, 2008;Rovira and Aramburu, 1998;Ylmaz et al, 2005;Birisik et al, 2008;Svoboda and Polak, 2008;Menzel et al, 2003;Boulila and Marrakchi, 2001;Chandel et al, 2008;Kapoor and Handa;Sanchez-Perez et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

Mass Screening of Temperate Fruit Germplasm against Major Viruses for developing a ‘Clean Stock’ Programme in Himachal Pradesh

Handa¹,

Kapoor²

2018

Int.J.Curr.Microbiol.App.Sci

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Section: Resultsmentioning

confidence: 99%

Mass Screening of Temperate Fruit Germplasm against Major Viruses for developing a ‘Clean Stock’ Programme in Himachal Pradesh

Handa¹,

Kapoor²

2018

Int.J.Curr.Microbiol.App.Sci

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“…Fiore et al [10] recognized that this group was outside of the classical phylogenetic scheme represented by PV96, PV32, and PE5 clusters; but at the same time, they stated that, based on the use of an alternative statistical approach, the four-cluster likelihoodmapping method, these accessions were considered as part of PV96 phylogroup. Gradually, the number of accessions increased including those Indian accessions described by Chandel et al [53][54][55]. Based on a careful phylogenetic study of the coat protein-encoding gene of PNRSV, Boulila [15] stressed that a distinct phylogroup emerged due possibly to different evolutionary traits.…”

Section: Discussionmentioning

confidence: 99%

Evidence for Recombination in the Emerged Phylogroup SW6 of Prunus Necrotic Ringspot Virus and Estimate of Selection Pressure Acting on Capsid Protein of Affiliated Members

Boulila¹

2017

JAMB

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Abstract. Recombination plays a key role in virus evolution. Except a limited number of works carried out so far on recombination in Prunus necrotic ringspot virus (PNRSV) genome, knowledge is still poor. Numerous phylogenetic studies particularly those based on capsid (CP) and movement protein (MP) of PNRSV showed that all accessions described so far split into three major phylogroups, i.e., PV96, PV32, and PE5. With the recent characterization of Chilean and Indian isolates which were phylogenetically closely related to the oldest member, i.e., SW6 (AF013287) from USA, a new phylogroup baptized SW6, emerged since it was outside of the evolutionary behavior of the classical PV96, PV32, and PE5 clusters. In order to understand how it evolved, six computerized methods were used to detect potential recombination in the CP sequences of nine components forming SW6 phylogroup. It was clearly demonstrated that various members underwent recombination. Additionally, networked relationships among them proved that numerous incompatibilities occurred in these sequences illustrated by the presence of boxes in the network implying therefore the possibility of recombination. According to inferred network, several sequences contained conflicting signals as illustrated by various splits and edges with special reference to Chilean (EF565255, EF565256), polish (AF332614) and Indian (AM494934) isolates. It is worth noting that the phylogenetic network provided a different clustering compared to the bifurcating tree. In fact, three distinct groups were delineated with SplitsTree4 software instead of a globally homogenous ensemble generated by MEGA7 algorithm. Furthermore, this study pointed out that members of SW6 phylogroup were the only recombinants among 205 tested accessions confirming that SW6 should be considered as the fourth phylogenetic group of PNRSV. On the other hand, CP was predominantly under purifying selection. However, positive selection was evidenced particularly in the C-terminal region of CP comprising part of dimerization region (DR). Such adaptive selection was exerted on a CP segment of AM494934 accession exchanged by recombination.

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“…In earlier studies in India, PNRSV is reported on peach apple and nectarine (Chandel et al 2007(Chandel et al , 2008a. Hence, further surveys were conducted on different stone fruit-growing areas in HP (Solan, Chamba, Sirmour, Kullu, and Palampur) and J&K to check the incidence of PNRSV, viral symptoms were observed on leaves of plum, cherry and almond.…”

Section: Surveys and Sample Collectionmentioning

confidence: 99%

Detection ofPrunus necrotic ring spot virusin plum, cherry and almond by serological and molecular techniques from India

Chandel

Rana

Hallan

et al. 2011

Archives Of Phytopathology And Plant Protection

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Stone fruits are cultivated in the temperate and sub-temperate regions of India. During surveys in stone fruit growing areas, viral symptoms were observed in almond, cherry and plum. These samples were brought to the laboratory for further detection at serological and molecular levels to check the presence of virus. In the present study, incidence of PNRSV is reported on plum (Prunus domestica), almond (Prunus dulcis) and cherry (Prunus avium) using serological and molecular techniques. Coat protein gene of PNRSV was amplified from almond, cherry and plum. This is the first molecular evidence of PNRSV on these stone fruits reported from India.

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Incidence of Prunus necrotic ring spot virus on Malus domestica in India

Cited by 23 publications

References 9 publications

Mass Screening of Temperate Fruit Germplasm against Major Viruses for developing a ‘Clean Stock’ Programme in Himachal Pradesh

Mass Screening of Temperate Fruit Germplasm against Major Viruses for developing a ‘Clean Stock’ Programme in Himachal Pradesh

Evidence for Recombination in the Emerged Phylogroup SW6 of Prunus Necrotic Ringspot Virus and Estimate of Selection Pressure Acting on Capsid Protein of Affiliated Members

Detection ofPrunus necrotic ring spot virusin plum, cherry and almond by serological and molecular techniques from India

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