Incorporating Lipids into Boar Sperm Decreases Chilling Sensitivity but Not Capacitation Potential1

He, L.; Bailey, Janice L.; Buhr, M.M.

doi:10.1095/biolreprod64.1.69

Cited by 57 publications

(50 citation statements)

References 63 publications

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“…He et al (2001) incubated fresh boar sperm with small unilamellar liposomes composed of five specific phospholipids selected to improve membrane resistance to cryopreservation. This approach successfully resulted in liposome fusion with the sperm and subsequent lipid transfer.…”

Section: How Can Sperm Be Protected?mentioning

confidence: 99%

Semen cryopreservation: Successes and persistent problems in farm species

Bailey

Morrier²,

Cormier³

2003

Can. J. Anim. Sci.

121

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Bailey, J., Morrier, A. and Cormier. N. 2003. Semen cryopreservation: Successes and persistent problems in farm species. Can. J. Anim. Sci. 83: 393-401. Artificial insemination has arguably been the most important practice contributing to the advancement of animal production. The numerous advantages of artificial insemination are augmented when the semen is cryopreserved and conserved for long periods. Unfortunately, the utility of cryopreserved semen is limited because for most mammals, even cattle, a considerable proportion of sperm loose their fertility during freezing-thawing. In many species, this loss of fertility is substantial, rendering cryopreserved semen impractical for routine use. Our goals are to characterize the nature of the sublethal sperm damage caused by cryopreservation, and to develop a method to improve the functional fertility of thawed semen. This review describes our theory that sperm are prematurely activated by cryopreservation ("cryo-capacitation") and discusses strategies for preventing this damage.Key words: Sperm, artificial insemination, capacitation, fertility Bailey, J., Morrier, A. et Cormier, N. 2003. La cryoconservation de la semence : les succès et les problèmes persistants chez les espèces de la ferme. Can. J. Anim. Sci. 83: 393-401. L'insémination artificielle a grandement contribué à l'avancement des productions animales. Les nombreux avantages qu'offre cette technique sont augmentés lorsque la semence est cryoconservée et entreposée pour de longues périodes. Malheureusement, l'utilisation de la semence cryoconservée est limitée puisque chez la majorité des mammifères, incluant la vache, une large proportion des spermatozoïdes perdra leur motilité pendant le processus de congéla-tion-décongélation. Chez plusieurs espèces, cette perte de fertilité est suffisamment substantielle pour rendre impraticable l'utilisation routinière de la semence cryoconservée. Les objectifs de notre laboratoire portent sur la caractérisation des dommages sous létaux subis par les spermatozoïdes lors de la cryoconservation et le développement de méthodes permettant d'améliorer la fertilité des spermatozoïdes décongelés. Cette revue décrit notre théorie selon laquelle les spermatozoïdes seraient prématurément activés par la cryoconservation ("cryo-capacitation"). Différentes stratégies permettant de prévenir ce dommage y sont également abordées.

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Section: How Can Sperm Be Protected?mentioning

confidence: 99%

Semen cryopreservation: Successes and persistent problems in farm species

Bailey

Morrier²,

Cormier³

2003

Can. J. Anim. Sci.

121

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“…The characteristic lability of the boar spermatozoon during cooling (Mazur 1963, Pursel et al 1973, Watson et al 1981 and the specific lipid composition of its membrane (Holt & North 1984, Bwanga 1991, Maxwell & Johnson 1997, Johnson et al 2000, He et al 2001 affect its survival post-thaw that, even when proven protocols are used (Westendorf et al 1975, Bwanga 1991, Eriksson & Rodríguez-Martínez 2000, Johnson et al 2000, He et al 2001, Carvajal et al 2004, still remains sub-optimal. Many attempts have been made to reduce the alterations produced in sperm cells during this process (Breininger et al 2005, Peñ a et al 2005, Bathgate et al 2006, but the results are still far from optimal.…”

Section: Introductionmentioning

confidence: 99%

Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs

Sancho

Casas²,

Ekwall³

et al. 2007

Reproduction

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This study evaluated the effects of cooling, freezing and thawing on the plasma membrane integrity, kinetics and expression of two sugar transporters glucose transporter-3 and -5 (GLUT-3 and GLUT-5) in spermatozoa from Iberian boars. Semen samples were collected twice weekly from eight young, fertile Iberian boars of the 'Entrepelado' and 'Lampiñ o' breeds. The samples were suspended in a commercial extender and refrigerated to 17 8C for transport to the laboratory (step A), where they were further extended with a lactose-egg yolk-based extender and chilled to 5 8C (step B) prior to freezing in the presence of glycerol (3%). Spermatozoa were assessed for plasma membrane integrity and sperm motility at each of the steps, including post-thaw (step C). Aliquots were also prepared for immunocytochemical localisation of the sugar transporters (fixed and thin smears for transmission and scanning electron microscopy levels respectively) and for SDS-PAGE electrophoresis and subsequent western blotting, using the same antibodies (rabbit anti-GLUT-3 and anti-GLUT-5 polyclonal antibodies). The results showed lower percentages of progressively motile spermatozoa at step C in both breeds, while the percentage of live spermatozoa was significantly lower only in the 'Entrepelado' breed. The results obtained from electron microscopy clearly showed that Iberian boar spermatozoa expressed the hexose transporters, GLUT-3 and GLUT-5. The pattern of expression, in terms of location and concentration, was characteristic in each case but, in the case of isoform GLUT-5, it remained constant during the different steps of freezing-thawing protocol. These results indicate that cryopreservation affects the status of sperm cells of Iberian boars by altering the distribution of some membrane receptors and decreasing the percentage values of parameters linked to sperm quality.

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“…The acrosome status of sperm was investigated using the method of He et al [28]. Briefly, sperm cells were fixed and permeabilised in absolute ethanol.…”

Section: Acrosome Stainingmentioning

confidence: 99%

Progesterone significantly enhances the mobility of boar spermatozoa

Campbell¹,

Savage²,

Madamidola³

et al. 2013

Bidiscovery

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Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4%, +100nM progesterone nonreacted = 47±10%). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner.

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Incorporating Lipids into Boar Sperm Decreases Chilling Sensitivity but Not Capacitation Potential1

Cited by 57 publications

References 63 publications

Semen cryopreservation: Successes and persistent problems in farm species

Semen cryopreservation: Successes and persistent problems in farm species

Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs

Progesterone significantly enhances the mobility of boar spermatozoa

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