Increase in Bcl2 expression of penile and prostate cells of Sprague Dawley male rats following treatment with buceng (combination of Pimpinella alpina molk with Eurycoma longifolia Jack)

Abstract

Background: Treatment with buceng combination of Eurycoma longifolia Jack and Pimpinella alpine Molk has been proven to increase testosterone level, decrease apoptosis and caspase3 expression. Bcl2 is an antiapoptotic protein found in cytoplasm which inhibits cells apoptosis. This study was aimed to investigate the effect of buceng on Bcl2 expression on penile and prostate tissues of the rats. Methods: In this experimental study, 24 male Sprague Dawley rats of 90 days old, weighing ± 300 grams, were randomly assigned into four groups. Group A, normal rats. Group B, castrated rats and treated with buceng 100 mg/day, per oral (Cast-Bcg); Group C, castrated rats and treated with 2 ml of water as placebo against buceng (Cast-Plac). Group D, castrated rats, treated with mesterolone 6.75 mg/day, per oral, as exogenous testosterone (Cast-Mest). All rats were treated for 30 days. Manova test was used to analyze the different expression of Bcl2 among groups with significance level at p ≤ 0.05. Results: Castration was associated with significant decrease of Bcl2 expression in the penile and prostate tissues (53.0 and 50.9%, respectively) compared to normal rats (82.6 and 84.2%, respectively, p < 0.001). Treatment with mesterolone reversed Bcl2 expression (77.1 and 78.1%) to a near normal level. The same level of Bcl2 expression was also observed with buceng treatment (73.8 and 78.2%).Conclusion: The treatment with buceng could enhance Bcl2 expression in penile and prostate tissues, comparable to normal rats and mesterolone treated rats.

Comparison of Bax and Caspase-3 Protein Expression in Liver Cells following UVB Irradiation for 7 days and Treatment of Pimpinella alpina Molk during 7 and 15 days

“…14,15 An imbalance between proapoptotic (Bax, Bad, Bak, Bid, and Bcl-Xs) and antiapoptotic protein (Bcl-2, Bcl-XL, Bag-1, and Bcl-W) capability is a prominent characteristic of liver injury. 16 Flavonoids, is a polyphenols secondary metabolite resulted from plants that have been proven capable of inhibiting and or inducing cellular apoptosis, 1,17,18 can be utilized to maintain protein pro and antiapotosis in balance condition. It is plausible, considering polyphenols are able to counteract oxidative stress by scavenging the excess of ROS, therefore suppress the oxidative stress responsive gene and cell proliferation can be inhibited.…”

“…There are growing evidences that PaM treatment was proven able to increase testosterone level, antioxidant concentration, and reduced apoptosis in penile, prostate, and hepatocyte cells in rat's model. 1,18 Apoptosis which is triggered by UVB irradiation may activate and stabilizes p53 in nucleus and cytoplasm. Consequently, p53 drive intrinsic and extrinsic apoptotic pathway through amplifying the apoptotic signal consisting of apaf 1, cell death receptor Fas (CD95), PUMA, Bax, and caspases3 mediated by activation of stress activated protein kinases (SAPK).…”

“…Consequently, p53 drive intrinsic and extrinsic apoptotic pathway through amplifying the apoptotic signal consisting of apaf 1, cell death receptor Fas (CD95), PUMA, Bax, and caspases3 mediated by activation of stress activated protein kinases (SAPK). 19 PaM is a botanical product contained flavonoids, constituting a potential antioxidant, radical scavenger, and metal ion chelator agent 1,18 . Accordingly, PaM is able to inhibit apoptosis mediated by reducing ROS Level and oxidative stress in any cells and tissues.…”

“…Accordingly, PaM is able to inhibit apoptosis mediated by reducing ROS Level and oxidative stress in any cells and tissues. 1,18 There are some evidences that the rate of aging process in various organ is prominently determined by the rate of apoptosis. Low rate of apoptosis result in dysfunctional cells, by contrast high rate of apoptosis may induce tissues degeneration.…”

Treatment of Pimpinella Alpina Molk Improve Oxidative stress and Inhibit Liver Cellular Apoptosis in Rats Following UVB Irradiation: Is there Any Correlation?

“…29,30 In intrinsic apoptosis pathway, the DNA damages induce mitochondrial permeability transition pore complex, which controls mitochondrial membrane potential to release cytochrome c. In concert with apaf-1, procaspase 9 , and ATP, cytochrome c forms apoptosome death complex (ADD), that proteolitically convert procaspase9 to caspase9, and then activate caspase7,6 and caspase3 resulting in DNA fragmentation and cell death. On the other hand, in extrinsic apoptosis UVB and ROS induces 32,33 Indeed those PaM effect were unlikely through antioxidant capacity instead of hormone stimulation effect in castrated male rats. It was possible, because PaM contained stigmasterol, is a natural weak androgen that believed as androgen precursor.…”