Increase in Lipid Content in Potato Tubers Modified by 14-3-3 Gene Overexpression

Abstract

Recently, transgenic potato plants were created with overexpression of the 14-3-3 protein derived from Cucurbita pepo. Detailed analysis of those plants suggested that the function of the isolated 14-3-3 isoform is in the control of carbohydrate and lipid metabolism in the plants. 14-3-3 protein overexpression gave rise to an increase in soluble sugar and catecholamine contents in both leaves and tubers. It is proposed that 14-3-3 protein affects carbohydrate metabolism in potato plants via regulation of catecholamine synthesis. Furthermore, genetically modified potato tubers with 14-3-3 protein overexpression showed changes in lipid content and composition. The transgenic potato tubers contained 69% more total fat compared to the wild-type plant. Separation of tuber lipids into polar and nonpolar fractions revealed that the transgenic potato tubers contained almost 3 times more nonpolar lipids than the control. Analysis of fatty acid composition, conducted by the means of gas chromatography, showed that linoleic acid was the main fatty acid present in the tubers of both modified and control potato plants. In the nonpolar fraction of the fat of the transgenic tubers the unsaturated fatty acids exhibited a higher participation in the sum of all fatty acids.

Fatty Acids and Bioactive Lipids of Potato Cultivars: An Overview

“…The result of the fatty acid analysis of TL extracted from wild type D and GMP with overexpression of 14-3-3 protein J2 26 showed that the percentage of oleic, linoleic and linolenic acids is in great agreement with the published reports of Kolbe et al 32 and Trevini et al 33 . The content of linoleic acid, the main fatty acid of potato, was approximately 49 .…”

“…Fatty acid profiling showed that linoleic acid was the main fatty acid of both GMP and non-genetically modified potato NGMP . In the non-polar fraction of lipids from GMP, the unsaturated fatty acids were found in higher levels 26,27 .…”

“…Although the intake of potato lipids is minimal due to its small content, the large portion of potatoes in the daily food rations in many countries significantly increased the daily consumption of unsaturated fatty acids such as linoleic 32.1 mg/100 g and linolenic acids 22.7 mg/100 g 25,26 . Thus, the cultivation of potato species, which accumulates more lipids in their tubers, would be an interesting task.…”

Identification of differentially expressed genes in seeds of two near-isogenic Brassica napus lines with different oil content

“…The direct evidence of role of 14-3-3 in lipid synthesis came from the genetically modiWed potato tubers with overexpressing 14-3-3 protein showed changes in lipid content and composition (Prescha et al 2001). However, the mechanism of this alteration is still unknown.…”

Effect of feeding potato tubers modified by 14-3-3 protein overexpression on metabolism and health status of rats

“…The potatoes also contained somewhat less crude fibre and more fat. A positive correlation between increased synthesis of 14-3-3 protein and a higher concentration of fat in potato tubers was also shown by Prescha et al (2001). It was also found that a significant increase in the nonpolar lipid fraction was associated with the overexpression of this protein.…”